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In vitro conservation of diploid banana accessions Scientia Agricola
Oliveira,Roberto Pedroso de; Silva,Sebastião de Oliveira e; Silva,Keuder Magalhães da; Silveira,Daniela Garcia.
A protocol for the in vitro conservation of diploid banana accessions based on lower temperature for culture environment was developed. Twenty four genotypes, four clones per genotype were studied. After disinfection, shoot tips (0.5 cm diameter x 0.5 cm height) were established in vitro and submitted to multiplication induced by benzylaminopurine. Twelve explants (0.6 cm diameter x 1.2 cm height) from each accession were transferred to the MS medium supplemented with 20 g L-1 sucrose and 2 g L-1 Phytagel, without growth regulators, under light intensity of 2000 lux, 16-hour photoperiod, at temperatures of: 17 ± 2ºC, 22 ± 2ºC and 26 ± 2ºC. The development of plantlets was evaluated during 450 days and the survived plantlets were acclimated under greenhouse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa spp.; Germplasm; In vitro conservation.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000200008
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Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa. Repositório Alice
PACHECO, G.; GAGLIARDI, R.F.; COGLIATTI, M.B.; MANHÃES, H.B.; CARNEIRO, L.A.; VALLS, J.F.M.; MANSUR, E..
The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation; Amendoim; Conservação in vitro; Micropropagação.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/118384
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Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa PAB
Pacheco,Georgia; Gagliardi,Rachel Fátima; Cogliatti,Mariana Buturini; Manhães,Harlen Barreira; Carneiro,Leonardo Alves; Valls,José Francisco Montenegro; Mansur,Elisabeth.
The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000100023
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Micropropagation and medium-term conservation of Rosa pulverulenta - doi: 10.4025/actasciagron.v33i2.10279 Agronomy
Kavand, Somayeh; Agricultural Biotechnology Research Institute of Iran; Kermani, Maryam Jafarkhani; Agricultural Biotechnology Research Institute of Iran; Haghnazari, Ali; Agricultural Biotechnology Research Institute of Iran; Khosravi, Pegah; Agricultural Biotechnology Research Institute of Iran; Azimi, Mohamad Reza; Agricultural Biotechnology Research Institute of Iran.
In Iran, a large number of Rosa species have been exposed to extinction and therefore preservation techniques are necessary to safeguard their future. In the present investigation, the objectives were to optimize the micropropagation and medium-term conservation of one of these wild species, Rosa pulverulenta. At proliferation stage, the maximum number of new leaves (9.6) were produced on the medium containing 4 µM BAP + 0 µM GA3, whereas the maximum number of axillary shoots (4.1) were observed in the medium containing 4 µM BAP + 3 µM GA3. The results for rooting experiments suggested that the highest increase in stem height (49.5 mm) at the acclimatization stage was observed in plantlets treated with 1 µM...
Tipo: Laboratory analysis Palavras-chave: In vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta in vitro conservation; Micropropagation; Proliferation; Recovery; Rooting; Rosa pulverulenta.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/10279
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Short-term storage in vitro and large-scale propagation of grapevine genotypes. Repositório Alice
SILVA, R. de C; LUIS, Z. G.; PEREIRA, J. E. S..
The objective of this work was to evaluate the large?scale propagation of grapevine genotypes after short?term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short?term storage, the shoots were propagated in up to five successive subcultures, to assess the large?scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short?term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ex situ conservation; In vitro conservation; Minimal growth; Vitis.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/930351
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Short-term storage in vitro and large-scale propagation of grapevine genotypes PAB
Silva,Rafael de Carvalho; Luis,Zanderluce Gomes; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vitis; Germplasm; Ex situ conservation; In vitro conservation; Micropropagation; Minimal growth.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000300005
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Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification PAB
Nogueira,Gabriela Ferreira; Pasqual,Moacir; Scherwinski-Pereira,Jonny Everson.
The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum; In vitro conservation; PVS2; Regeneration.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100014
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Cryopreservation of the mangaba tree (Hancornia speciosa Gomes): a protocol for long-term storage Agronomy
Santos, Paulo Augusto Almeida; Paiva, Renato; Silva, Luciano Coutinho; Souza, Ana Cristina de; Santana, Marlucia Cruz de; Silva, Diogo Pedrosa Corrêa da.
The aim of this study was to evaluate the efficiency of vitrification and droplet vitrification for the cryopreservation of Hancornia speciosa shoot tips. The shoot tips were subjected to four different periods of exposure (15, 30, 45 and 60 min.) to plant vitrification solution 2 (PVS2) before plunging into liquid nitrogen. We evaluated the regrowth of H. speciosa shoot tips that were cryopreserved by the classical vitrification technique and by the droplet vitrification technique. Shoot tips were submitted to different periods of pre-culture (absence, 24 or 48h) in a medium containing 0.3 M sucrose prior to cryopreservation. With a PVS2 exposure period of 60 min., significant differences were observed between the two techniques used in this study with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.00.00.00-4 - ciências agrárias; 2.03.03.00-9 - fisiologia vegetal; Native species; In vitro conservation; Recalcitrant seeds; Plant genetic diversity conservation; Vitrification; Droplet vitrification Conservação in vitro; Criopreservação vegetal.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19573
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Ex situ potential conservation of ipecacuanha ( Psychotria ipecacuanha (Brot.) Stokes . ) , a critically endangered medicinal plant species Acta Agron. (Palmira)
Urrea Trujillo,Aura Inés; Botero Giraldo,Catalina; Naranjo Gómez,Esther Julia.
Abstract Psychotria ipecacuanha is a medicinal plant endangered in Colombia, due to the indiscriminate exploitation suffered several decades ago for its therapeutic importance. Its great medicinal value is due to emetine production and its derivatives, mainly in the roots. Given these concerns, the aim of this research was to evaluate different parameters for in vitro conservation of P. ipecacuanha by a limited plant growth, using as in vitro explants plant nodes. Plant growth response to 18, 23 and 25°C with and without addition to MS basal culture medium 15 and 30 gL-1 of mannitol osmotic regulator was evaluated. Registration of plant survival data (%), plant height (cm), plant development of axillary shoots, number of leaves, callus formation and vigour...
Tipo: Journal article Palavras-chave: In vitro conservation; Plant limited growth; Osmotic regulator; Recovery phase; Vigour.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000400598
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Cryopreservation of banana’s cv Grand Naine in vitro rhizomes Anais da ABC (AABC)
LONDE,LUCIANA C.N.; VENDRAME,WAGNER A.; SANAEI,MASSY; OLIVEIRA,ALEXANDRE B. DE.
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa acumminata; In vitro conservation; Cryoprotectants; Pre-cooling.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100495
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