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Registros recuperados: 8
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Secretion of laccase and manganese peroxidase by Pleurotus strains cultivate in solid-state using Pinus spp. sawdust BJM
Camassola,Marli; Rosa,Letícia O. da; Calloni,Raquel; Gaio,Tamara A.; Dillon,Aldo J.P..
Pleurotus species secrete phenol oxidase enzymes: laccase (Lcc) and manganese peroxidase (MnP). New genotypes of these species show potential to be used in processes aiming at the degradation of phenolic compounds, polycyclic aromatic hydrocarbons and dyes. Hence, a screening of some strains of Pleurotus towards Lcc and MnP production was performed in this work. Ten strains were grown through solid-state fermentation on a medium based on Pinus spp. sawdust, wheat bran and calcium carbonate. High Lcc and MnP activities were found with these strains. Highest Lcc activity, 741 ± 245 U gdm-1 of solid state-cultivation medium, was detected on strain IB11 after 32 days, while the highest MnP activity occurred with strains IB05, IB09, and IB11 (5,333 ± 357; 4,701...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pleurotus; Laccase; Manganese peroxidase; Mushroom production; Biotechnological applications.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100031
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Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability BJM
Fonseca,María Isabel; Tejerina,Marcos Raúl; Sawostjanik-Afanasiuk,Silvana Soledad; Giorgio,Ernesto Martin; Barchuk,Mónica Lucrecia; Zapata,Pedro Darío; Villalba,Laura Lidia.
Abstract Oxidative enzymes secreted by white rot fungi can be applied in several technological processes within the paper industry, biofuel production and bioremediation. The discovery of native strains from the biodiverse Misiones (Argentina) forest can provide useful enzymes for biotechnological purposes. In this work, we evaluated the laccase and manganese peroxidase secretion abilities of four newly discovered strains of Trametes sp. that are native to Misiones. In addition, the copper response and optimal pH and temperature for laccase activity in culture supernatants were determined.The selected strains produced variable amounts of laccase and MnP; when Cu2+ was added, both enzymes were significantly increased. Zymograms showed that two isoenzymes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: White rot fungi; Trametes; Laccase; Manganese peroxidase; Cu2+ response.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200287
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Ligninases production by Basidiomycetes strains on lignocellulosic agricultural residues and their application in the decolorization of synthetic dyes BJM
Gomes,Eleni; Aguiar,Ana Paula; Carvalho,Caio César; Bonfá,Maricy Raquel B.; Silva,Roberto da; Boscolo,Mauricio.
Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccase; Manganese peroxidase; Lignin peroxidase; Basidiomycetes; Decolorization; Synthetic dye.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100005
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Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review Electron. J. Biotechnol.
Maciel,Márcia Jaqueline Mendonça; Silva,Ademir Castro e; Ribeiro,Helena Camarão Telles.
Ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. The demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. Their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. This paper reviews the applications of ligninolytic...
Tipo: Journal article Palavras-chave: Laccase; Lignin peroxidase; Manganese peroxidase; White-rot fungi.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600014
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Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium BABT
Ntwampe,Seteno; Chowdhury,Faysol; Sheldon,Marshall; Volschenk,Heinrich.
The production of extracellular enzymes is gaining momentum as commercial interests seek alternative ways to improve the productivity in the biotechnology and pharmaceutical industries. Early research studies looked at improving batch bioreactor operational challenges; however, the use of continuous cultures was indicated to be favourable. This led to a new approach developed to produce extracellular enzymes continuously using fixed-film bioreactors from biofilms immobilised on polymeric and inorganic membranes. In this review, the performance of P. chrysosporium biomass, evaluated in terms of ligninase production using different bioreactor operation conditions, is highlighted. Furthermore, the limitations related to the implementation of optimised batch...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerochaete chrysosporium; Lignin peroxidase; Manganese peroxidase; Dissolved oxygen; Bioreactors.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500008
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Purification and Partial characterization of manganese peroxidase from Bacillus pumilus AND Paenibacillus sp. BJM
Oliveira,Patrícia Lopes de; Duarte,Marta Cristina Teixeira; Ponezi,Alexandre Nunes; Durrant,Lúcia Regina.
The production of manganese peroxidase (MnP) from Bacillus pumilus and Paenibacillus sp. was studied under absence and presence of the inducers indulin AT, guayacol, veratryl alcohol, lignosulfonic acid and lignosulfonic acid desulfonated. Indulin AT increased the activity of B. pumilus MnP up to 31.66 U/L after 8 h, but no improve was observed for Paenibacillus sp., which reached maximum activity (12.22 U/L) after 20 h. Both MnPs produced by these microorganisms were purified in phenyl sepharose resin and the proteins from crude extracts were eluted in two fractions. However, only the first fraction of each extract exhibited MnP activities. Tests in different pH and temperature values, from pH 5.0 to pH 10.0 and 30 ºC to 60 ºC, respectively, were carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus pumilus; Paenibacillus sp.; Manganese peroxidase; Purification; Characterization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400012
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Solid State production of manganese peroxidases using arecanut husk as substrate BABT
Rajan,Akhila; Kurup,Jayalakshmi Gopinatha; Abraham,Tholath Emilia.
The lignocellulosic biomass from arecanut husk (Areca catechu Linnaeus) was evaluated as a new substrate for cultivation of Phanerochaete chrysosporium and Phanerochaete sp for solid state fermentation of manganese peroxidase (MnP). Arecanut had a moisture content of 79.84 % for ripe nut husk whereas green nut husk had 68.39 % moisture and a pH of 5.0, 3.0 and 7.0 for raw, ripe and dry husk. Reducing sugar content was 14.31, 19.21 and 1.77(mg/g of husk) for raw, ripe and dry nut husk, respectively. Non reducing sugar was 1.04(mg/g of husk) for raw and 0.68 (mg/g of husk) for dry husk. Solid state fermentation carried out at different pH showed optimum enzyme production at pH 6.0 (52.60 IU/g) for P.chrysosporium and pH 5.0 (44.08 IU/g) for Phanerochaete sp....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arecanut husk; Solid state fermentation; Manganese peroxidase; Phanerocheate chrysosporium; Phanerocheate sp.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300008
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Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus BJM
Yehia,Ramy Sayed.
Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81UmL-1, specific activity 78 U mg-1 with purification fold of 130 and recovery 1.2% of the crude enzyme. SDS-PAGE indicated that the pure enzyme have a molecular mass of approximately 42 kDa. The optimum pH was between 4-5 and the optimum temperature was 25 ºC. The pure MnP activity was enhanced by Mn2+,Cu2+,Ca2+ and K+ and inhibited by Hg+2 and Cd+2.H2O2 at 5 mM enhanced MnP activity while at 10 mM inhibited it significantly. The MnP-cDNA encoding gene was sequenced and determined...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Manganese peroxidase; Aflatoxin B1; Pleurotus ostreatus; Purification.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100018
Registros recuperados: 8
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