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Registros recuperados: 7
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Molecular characterization of penaeidins from two Atlantic Brazilian shrimp species, Farfantepenaeus paulensis and Litopenaeus schmitti ArchiMer
Barracco, Margherita; De Lorgeril, Julien; Gueguen, Yannick; Bachere, Evelyne.
We report here the molecular cloning of new members of the penaeidin family from two Atlantic penaeids from Brazil, Litopenaeus schmitti and Farfantepenaeus paulensis. The presence of penaeidins in the granular hemocytes of both shrimps was first evidenced by immunofluorescence, using polyclonal antibodies raised against L. vannamei penaeidin Litvan PEN3-1. cDNAs from the hemocytes of both Brazilian species were obtained by reverse transcription and the sequences encoding penaeidins were amplified by PCR, using primers based on penaeidin consensus sequences. Five penaeidin clones were obtained. According to the international penaeidin classification (PenBase, http://www.penbase.immunaqua.com), the deduced amino acid sequences of two clones from L. schmitti...
Tipo: Text Palavras-chave: Immuno detection; Molecular cloning; Litopenaeus schmitti; Farfantepenaeus paulensis; Brazilian penaeid shrimps; Hemocytes; Antimicrobial peptides; Penaeidins.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3582.pdf
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An effective method for cloning of partial MADS-box genes related to flower development in groundnut Open Agri
Yuan, M..
Palavras-chave: Genes; PCR; DNA; Cloning; Transcription factors; Genomes; Transcription; Genetic structures; Nucleotides; Molecular cloning.
Ano: 2005 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/3213
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Expression of a Haemonchus contortus cysteine protease in the baculovirus system Electron. J. Biotechnol.
Miranda-Miranda,Estefan; Murillo-Sánchez,María Hortensia; Cossío-Bayúgar,Raquel.
A Haemonchus contortus recombinant Cysteine Protease (CP) was expressed in the baculovirus system. The CP gene was isolated by PCR from H. contortus cDNA, the PCR amplicon was cloned downstream to the polihedrin promoter within a bacterial expression vector, Sf9 insect cells were used for simultaneous co-transfection with the CP-vector and baculovirus naked DNA, which originated recombinant viruses by homologous recombination capable to express recombinant CP in an insect cell culture. A recombinant protease was identified as a fusion protein with a Ni lithium affinity 6XHis group. Recombinant CP was purified by affinity chromatography to obtain active recombinant protease identified by H. contortus experimentally infested ovine sera on a western blot as a...
Tipo: Journal article Palavras-chave: Haemonchosis; Molecular cloning; Recombinant protease; Synthetic substrates; Western blot.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200007
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Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory AgEcon
Gallicia, L.; Nurit, E.; Rosales, A.; Palacios-Rojas, N..
Tipo: Report Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009 URL: http://purl.umn.edu/56177
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Molecular cloning and functional characterization of a catalase gene from a Bacillus safensis strain isolated from an oil-impacted Brazilian mangrove sediment. Repositório Alice
OTTONI, J. R.; BELOTI, L. L.; SANTIAGO, A. S.; COSTA, B. Z.; MELO, I. S. de; SOUZA, A. P.; MARSAIOLI, A. J.; OLIVEIRA, V. M..
2014
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Mangrove; Bacillus safensis; Molecular cloning; Catalase.
Ano: 2014 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1010901
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Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning Electron. J. Biotechnol.
Cheng,Jingliang; Long,Yan; Khan,Asaduzzaman; Wei,Chunli; Fu,Shelly; Fu,Junjiang.
Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. The goal of this study was to develop sequence-characterized amplified region (SCAR) markers from the improved RAPD fragments for the genetic analysis of L. chinensis. Results The improved RAPD fragments from L. chinensis were cloned, sequenced and converted into stable SCAR markers. Sequencing of three cloned RAPD fragments revealed that the clone L7-16 consisted of 222 nucleotides (GenBank accession number KM235222), clone L9-6 consisted of 648...
Tipo: Journal article Palavras-chave: Genetic authentication; Molecular cloning; Random amplified polymorphic DNA; Sequence-characterized amplified region marker.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007
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A pigeonpea gene confers resistance to Asian soybean rust in soybean. Repositório Alice
KAWASHIMA, C. G.; GUIMARÃES, G. A.; NOGUEIRA, S. R.; MACLEAN, D.; COOK, D. R.; STEUERNAGEL, B; BAEK, J.; BOUYIOUKOS, C; MELO, B. do V. A.; TRISTÃO, G.; OLIVEIRA, J. C. de O.; RAUSCHER, G.; MITTAL, S.; PANICHELLI, L.; BACOT, K.; JOHNSON, E.; IYER, G.; TABOR, G.; WULFF, B. B. H.; WARD, E.; RAIRDAN, G. J.; BROGLIE, K. E.; WU, G.; ESSE, H. P. van; JONES, J. D. G.; BROMMONSCHENKEL, S. H..
Asian soybean rust (ASR), caused by the fungus Phakopsora pachyrhizi, is one of the most economically important crop diseases, but is only treatable with fungicides, which are becoming less effective owing to the emergence of fungicide resistance. There are no commercial soybean cultivars with durable resistance to P. pachyrhizi, and although soybean resistance loci have been mapped, no resistance genes have been cloned. We report the cloning of a P. pachyrhizi resistance gene CcRpp1 (Cajanus cajan Resistance against Phakopsora pachyrhizi 1) from pigeonpea (Cajanus cajan) and show that CcRpp1 confers full resistance to P. pachyrhizi in soybean. Our findings show that legume species related to soybean such as pigeonpea, cowpea, common bean and others could...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Ferrugem asiática; Phakpsora pachyrhizi; Melhoramento genético vegetal; Soja; Glycine max; Doença de planta; Variedade resistente; Fungo; Genótipo; Clonagem; Leguminosa com grão; Feijão; Guandu; Cajanus cajan; Plant breeding; Soybeans; Plant diseases and disorders; Fungi; Soybean rust; Beans; Pigeon peas; Molecular cloning; Fitomejoramiento; Semillas de soja; Enfermedades y desórdenes de las plantas; Roya de la soja; Frijoles; Guandul; Molecular clonación.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1048021
Registros recuperados: 7
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