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Registros recuperados: 10
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Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil Trop. Plant Pathol.
Almeida,Álvaro M. R.; Sakai,Junichi; Hanada,Kaoru; Oliveira,Thalita G.; Belintani,Priscila; Kitajima,Elliot W.; Souto,Eliezer R.; Novaes,Tanara G. de; Nora,Paulo S..
A virus was isolated from soybean (Glycine max) plants with symptoms of dwarfing and bud blight in Wenceslau Braz County, Paraná, Brazil. The host range and properties resembled those of Tobacco streak virus (TSV). The purified virus showed three peaks in a frozen sucrose gradient. Antiserum was produced and the virus was serologically related to TSV. Electron microscopy detected 28 nm spherical particles. Coat protein (CP) had a Mr of 29.880 Da. A fragment of 1028 nt was amplified, cloned and sequenced. One open reading frame with 717 nt was identified and associated to the CP. The CP gene shared 83% identity with the sequence of TSV CP from white clover (Trifolium repens) (GenBank CAA25133). This is the first report of the biological and molecular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nucleotide sequence; Elisa; Host range.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000400005
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Analise dos processsos de quebra, sintese e reparo do DNA durante a profase meiotica em Lilium spp. Infoteca-e
ALVES, R. M.; NASCIMENTO FILHO, F. J. do.
Celulas meioticas de lirio, microsporocitos, tem sido amplamente utilizadas para estudos bioquimicos combinados. O sincronismo natural das celulas meioticas, a duracao arelativamente longa da profase (2 a 5 dias) e a habilidade das celulas desenvolverem-se in vitro possibilitou analisar cada intervalo da sintese de DNA, separadamente.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Lillium; Brasil; Sao Paulo; Piracicaba; Species; Plants; Nucleotide sequence; DNA; Espécie; Meiose; Planta.
Ano: 1984 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/670465
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Sequenciamento de Nova Geração (NGS) de Urochloa mosambicensis: uma forrageira promissora para o semiárido. Repositório Alice
LEAO, U. S.; BUENO, L. G.; WILKE, D. V.; MAGGIONI, R.; SILVA, G. R. da; NEGREIROS, A. B.; DINIZ, F. M..
A forrageira Urochloa mosambicensis, conhecida vulgarmente por capim corrente, capim urocloa, capim gunia, grama de Sabi, é uma espécie de gramínea da família Poaceae originária da Rodésia (África) e foi introduzida no Brasil em 1922 para alimentação de animais criados no semiárido nordestino. Este estudo tem por objetivo realizar o sequenciamento de nova geração da espécie Urochloa mosambicensis para a mineração de regiões microssatélites a fim de serem utilizados em estudos de melhoramento desta importante forrageira.
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Capim corrente; Grama de Sabi; Capim Gunia; Microssatélite; Expresão genica; Urochloa mosambicensis; Capim Urochloa; Gramínea Forrageira; Genoma; Genomes; Nucleotide sequence; Plant genetics.
Ano: 2017 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080722
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Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001 BJMBR
Pires Neto,R.J.; Lima,D.M.; de Paula,S.O.; Lima,C.M.; Rocco,I.M.; Fonseca,B.A.L..
Dengue is a mosquito-borne viral infection that in recent decades has become a major international public health concern. Epidemic dengue fever reemerged in Brazil in 1981. Since 1990 more than one dengue virus serotype has been circulating in this tropical country and increasing rates of dengue hemorrhagic fever and dengue shock syndrome have been detected every year. Some evidence supports the association between the introduction of a new serotype and/or genotype in a region and the appearance of dengue hemorrhagic fever. In order to study the evolutionary relationships and possible detection of the introduction of new dengue virus genotypes in Brazil in the last years, we analyzed partial nucleotide sequences of 52 Brazilian samples of both dengue type...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dengue virus; Nucleotide sequence; E/NS1 region; Molecular epidemiology; Dengue fever; Dengue hemorrhagic fever.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000600005
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Nucleotide sequence, genomic organization and chromosome localization of 5S rDNA in two species of Curimatidae (Teleostei, Characiformes) Genet. Mol. Biol.
Santos,Lessandra Viviane de Rosa; Foresti,Fausto; Wasko,Adriane Pinto; Oliveira,Claudio; Martins,Cesar.
The 5S ribosomal DNA (5S rDNA) of higher eukaryotes is organized in repeat units of tandem arrays composed of a 5S rDNA coding region, conserved even among non-related taxa, and a variable non-transcribed spacer sequence (NTS). To contribute to knowledge on the organization and evolution of vertebrate 5S rDNA we used PCR, nucleotide sequencing, Southern blot hybridization and chromosome fluorescence in situ hybridization (FISH) to investigate 5S rDNA tandem repeats in the South American Curimatidae fish Steindachnerina insculpta and Cyphocharax modesta. 5S rDNA repeats of 180 base pairs (bp) from both species were PCR-generated and sequenced evidencing the shortest 5S rDNA monomer so far described in eukaryote species. Southern blotting revealed that both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyphocharax modesta; Steindachnerina insculpta; 5S rDNA; Chromosomes; FISH; Nucleotide sequence.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200009
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Comparative sequence analysis of the P-, M- and L-coding region of the measles virus CAM-70 live attenuated vaccine strain BJMBR
Santos,P.R.; Azevedo,M.L.B.; Borges,M.B.J.; Freire,M.S.; Nascimento,J.P.; Moraes,M.T.B..
Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Measles virus; Vaccine; Nucleotide sequence; RNA sequencing.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100005
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Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus Scientia Agricola
Spadotti,David Marques de Almeida; Wassano,Débora Targino; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo Aranha; Inoue-Nagata,Alice Kazuko.
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Potyvirus; Nucleotide sequence; Genetic diversity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000200187
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Biotin-labeled Genomic DNA Probe for the Detection of Theileria sergenti and its Nucleotide Sequence OAK
Tanaka, Masayuki; Matsuba, Takashi; Onoe, Sadao; Yonemichi, Hiromi; Okabe, Tatsuji; Sasaki, Norimasa; Sugimoto, Chihiro; Onuma, Misao.
Palavras-chave: DNA probe diagnosis; Nucleotide sequence; Theileria sergenti.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/162
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Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species Genet. Mol. Biol.
Wasko,Adriane P.; Severino,Fábio E.; Presti,Flávia T.; Poletto,Andréia B.; Martins,Cesar.
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actin gene; Amino acid residues; Gene structure; Nile tilapia; Nucleotide sequence; Oreochromis niloticus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010
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Genomic characterization of Brazilian hepatitis C virus genotypes 1a and 1b BJMBR
Parts of 5' non-coding (5' NC) and of E1 envelope regions of the hepatitis C virus (HCV) genome were amplified from sera of 26 Brazilian anti-HCV antibody-positive patients using the reverse transcription-polymerase chain reaction (RT-PCR). Fourteen samples were PCR positive with primers from the 5' NC region and 8 of them were also positive with primers from the E1 region. A genomic segment of 176 bp from the E1 region of 7 isolates was directly sequenced from PCR products. The sequences were compared with those of HCV strains isolated in other countries and the Brazilian isolates were classified by phylogenetic analysis into genotypes 1a and 1b. This could have a clinical importance since it has been shown that individuals infected with type 1 viruses...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Hepatitis C virus; Genotype 1a; Genotype 1b; Nucleotide sequence; Brazilian isolates.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300006
Registros recuperados: 10
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