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Registros recuperados: 25
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Osmoregulatory responses to dietary protein and water intake in the granivorous Zonotrichia capensis (Passerine, Emberizidae) RChHN
ALDEA,PATRICIA; SABAT,PABLO.
We studied the osmoregulatory responses of the granivore Zonotrichia capensis, acclimated to high- and low-protein diets as well as two levels of water intake. We tested whether Z. capensis has the ability to modify the proportion of nitrogenous waste in the excreta when protein intake and water intake varies. After 21 days of acclimation, plasma osmolality was not significantly affected by dietary treatment; however, excreta osmolality was higher in the high-protein group compared to the low-protein group. Nitrogenous wastes in Z. capensis are excreted mainly in the form of uric acid. The proportion of kidney devoted to medullary tissue was 40 % higher in dehydrated birds than in hydrated birds. Excreta osmolality was higher in dehydrated birds, and in...
Tipo: Journal article Palavras-chave: Granivory; Kidney; Nitrogenous waste; Osmoregulation; Passerines.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000400006
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The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax ArchiMer
Bossus, Maryline; Charmantier, Guy; Blondeau-bidet, Eva; Valletta, Bianca; Boulo, Viviane; Lorin-nebel, Catherine.
Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW,...
Tipo: Text Palavras-chave: ClC-3 chloride channel; Na+/K+-ATPase; Dicentrarchus labrax; Osmoregulation; Osmosensing.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00149/26044/25185.pdf
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Differential distribution of V-type H+-ATPase and Na+/K+-ATPase in the branchial chamber of the palaemonid shrimp Macrobrachium amazonicum ArchiMer
Boudour-boucheker, Nesrine; Boulo, Viviane; Charmantier-daures, Mireille; Grousset, Evelyse; Anger, Klaus; Charmantier, Guy; Lorin-nebel, Catherine.
V-H+-ATPase and Na+/K+-ATPase were localized in the gills and branchiostegites of M. amazonicum and the effects of salinity on the branchial chamber ultrastructure and on the localization of transporters were investigated. Gills present septal and pillar cells. In freshwater (FW), the apical surface of pillar cells is amplified by extensive evaginations associated with mitochondria. V-H+-ATPase immunofluorescence was localized in the membranes of the apical evaginations and in clustered subapical areas of pillar cells, suggesting labeling of intracellular vesicle membranes. Na+/K+-ATPase labeling was restricted to the septal cells. No difference in immunostaining was recorded for both proteins according to salinity (FW vs. 25 PSU). In the branchiostegite,...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+-ATPase; V-type H+-ATPase; Gills; Branchiostegite.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00202/31352/37901.pdf
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A transcriptomic approach of salinity response in the euryhaline teleost, Dicentrarchus labrax ArchiMer
Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois.
Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using...
Tipo: Text Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf
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Molecular characterisation of prolactin and analysis of extrapituitary expression in the European sea bass Dicentrarchus labrax under various salinity conditions ArchiMer
Boutet, Isabelle; Lorin Nebel, C; De Lorgeril, Julien; Guinand, B.
dAlthough prolactin has been demonstrated to be the main hormone involved in adaptation to dilute media in several freshwater teleosts, few studies have been conducted in marine teleosts. In the Mediterranean, the sea bass Dicentrarchus labrax inhabits environments ranging from the open sea to coastal lagoons, where salinity varies greatly. We characterised the prolactin (prl) gene and analysed its expression in two organs (gill and intestine) in D. labrax acclimated to either freshwater or seawater. A 2819 bp long sequence encompassing the prl gene and a part (282 bp) of the promoter were identified, and these comprised 5 coding exons separated by 4 introns. Prolactin was similarly expressed in fresh- and seawater adapted fish, although expression in...
Tipo: Text Palavras-chave: Prolactin; Phylogenctic analysis; MRNA expression; Osmoregulation; Intestine; Gill; Dicentrarchuslabrax.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2537.pdf
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Could a diet enriched with n-3 highly unsaturated fatty acids be considered a promising way to enhance the immune defences and the resistance of Penaeid prawns to environmental stress? ArchiMer
Chim, Liet; Lemaire, Pierrette; Delaporte, Maryse; Le Moullac, Gilles; Galois, Robert; Martin, Jean-louis.
The prawn Penaeus stylirostris (Stimpson), when fed for 28 days with n-3 highly unsaturated fatty acid (HUFA)-enriched feed pellets, demonstrated an enhanced resistance to variations in environmental parameters (a decrease in temperature and salinity over a 4-day period from 28 degreesC to 17 degreesC and from 35 parts per thousand to 10 parts per thousand respectively) and an improvement in their immune defence potential, i.e. increased agglutination titre of plasma and increased respiratory burst of haemocytes.
Tipo: Text Palavras-chave: Immunostimulation; Osmoregulation; Environment; Stress; Essential fatty acids; Crustaceans.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-606.pdf
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Physiological responses of two ecologically important Kenyan mangrove crabs exposed to altered salinity regimes OceanDocs
De Wachter, B.; Gillikin, D.P.; Tack, J..
The potential long-term effects of altered salinity regimes on the bioenergetics of two ecologically important Kenyan mangrove crabs, Neosarmatium meinerti de Man, 1887 and Neosarmatium smithi H. Milne Edwards, 1853 were investigated in light of recent findings suggesting that groundwater redirection may alter salinity regimes in Kenyan mangroves. Although changes in groundwater may cause only small increases in salinities, these changes would be chronic and may impact crab populations already living above their optimal salinity. To assess potential impacts, fundamental physiological processes and hemolymph components were measured on animals acclimated to 16‰, 32‰, 48‰ and 65‰ for 4 weeks in a field laboratory. For comparative purposes, crabs were also...
Tipo: Journal Contribution Palavras-chave: Ecophysiology; Salinity Tolerance; Energy Budget; Osmoregulation; Sesarmidae; Crab fisheries.
Ano: 2004 URL: http://hdl.handle.net/1834/693
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An economic evaluation of the osmoregulation gene technology to the Australian wheat industry AgEcon
Farquharson, Robert J.; Morgan, J.M.; Brennan, John P..
Episodes of rainfall irregularity and soil moisture deficit have focused attention on the widespread limitation of water supply on winter cereal crop production in Australia. This has motivated a number of efforts at breeding for improved drought tolerance. A recent example involves a cellular adaptation which mitigates water loss through solute accumulation (osmoregulation or osmotic adjustment). An assessment of the performance of osmoregulation yield response in the presence of climate change found that wheat cultivars with this gene are unlikely to be adversely affected by hotter and drier conditions across the wheat belt. The results of an economic evaluation of potential future innovations (wheat cultivars) from the osmoregulation gene technology are...
Tipo: Conference Paper or Presentation Palavras-chave: Economic evaluation; Wheat breeding; Osmoregulation; Crop Production/Industries; Research Methods/ Statistical Methods.
Ano: 2004 URL: http://purl.umn.edu/58446
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Incidence de la salinite sur les equilibres de distribution hydrique et ioniques et sur le metabolisme respiratoire dans le muscle blanc, le muscle rouge et la branchie chez le muge Chelon labrosus ArchiMer
Gallis, J; Piquemal, S; Lambert, J; Sergent, P; Rousseau, V.
Oxygen consumption, water, K super(+) and Na super(+) contents were measured in white muscle, red muscle and gill epithelium in the mullet Chelon labrosus) in relation with the duration of fresh water or sea water (1500mOsm) adaptation. The unit respiratory activities in gill and red muscle where respectively 5 and 3 timesmore elevated than in white muscle. The pericellular K super(+)/Na super(+) ratio of the 3 tissues decreased with the fresh water adaptation duration. In the gill, the intracellular K super(+)/Na super(+) ratio was stable while the parameter decreased strongly in the white muscle. The results are discussed in relation to energetic cost of homocellular regulation and homoepithelial regulation.
Tipo: Text Palavras-chave: Chelon labrosus; Mugilidae; Pisces; Gills; Muscles; Oxygen consumption; Respiration; Ions; Water balance; Osmoregulation; Salinity effects.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1251.pdf
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Digestive tract ontogeny of Dicentrarchus labrax: Implication in osmoregulation ArchiMer
Giffard, I; Charmantier, G; Grousset, E; Aujoulat, F; Castille, R.
The ontogeny of the digestive tract (DT) and of Na+/K+-ATPase localization was investigated during the early postembryonic development (from yolk sac larva to juvenile) of the euryhaline teleost Dicentrarchus labrax reared at two salinities: seawater and diluted seawater. Histology, electron microscopy and immunocytochemistry were used to determine the presence and differentiation of ion transporting cells. At hatching, the DT is an undifferentiated straight tube over the yolk sac. At the mouth opening (day 5), it comprises six segments: buccopharynx, esophagus, stomach, anterior intestine, posterior intestine and rectum, well differentiated at the juvenile stage (day 72). The enterocytes displayed ultrastructural features similar to those of...
Tipo: Text Palavras-chave: Osmoregulation; Na+/K+ ATPase; Immunolocalization; Fish larva; Enteric ionocyte.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-4653.pdf
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Evaporative water loss and dehydration during the night in hummingbirds RChHN
HARTMAN BAKKEN,BRADLEY; SABAT,PABLO.
Nectar-feeding birds oscillate between avoiding overhydration when they are feeding and preventing dehydration during fasts. Here, we examined how resting rates of total evaporative water loss (TEWL) and metabolic water production (MWP) influence water balance in the green-backed firecrown (Sephanoides sephanoides), a Chilean hummingbird. We hypothesized that a circadian rhythm in TEWL would assuage the dehydration risk that hummingbirds face during the night. However, we did not find support for this idea. In resting hummingbirds, rates of TEWL during the day (54 ±6 µLh-1,n = 8) and night (65 ± 12 h-1, n = 5) were similar. Rates of MWP were also similar between the day (22 ±3 µL h-1, n = 8) and night (23 ± 2 µL h-1, n = 5). MWP rates were significantly...
Tipo: Journal article Palavras-chave: Circadian rhythm; Evaporative water loss; Hummingbird; Osmoregulation; Water balance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2007000300001
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Adaptations biochimiques intestinales chez la truite: Influence de l'aliment et de la salinite sur quelques proprietes membranaires ArchiMer
Leray, C.
The authors report the results of physiological and biochemical experiments which confirm the important role player by fish (Salmo gairdneri ) intestinal mucosa in their osmoregulatory process. The ability of the dietary lipids to modulate this function is described. It was demonstrated that the ionic apical permeability of the enterocytes are related to their membrane lipid composition. The importance of the essential fatty acids is emphasized. The time course changes of the apical permeability are of value in improving sea water adaptation, similar conclusions are reported for Nacl supplementation.
Tipo: Text Palavras-chave: Salmonidae; Pisces; Biological membranes; Fatty acids; Diets; Osmotic adaptations; Biochemical phenomena; Intestines; Lipids; Nutrition; Salinity effects; Euryhalinity; Osmoregulation.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1249.pdf
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The Na+/K+/2Cl(-) cotransporter in the sea bass Dicentrarchus labrax during ontogeny: involvement in osmoregulation ArchiMer
Lorin, N; Boulo, Viviane; Bodinier, C; Charmantier, G.
This study combines a cellular and molecular analysis of the Na+/K+/2Cl(-) cotransporter (NKCC) to determine the osmoregulatory role of this protein in different tissues during the ontogeny of the sea bass. We have characterized the complete sequence of the NKCC1 isoform isolated from the sea bass gills and have identified, by immunofluorescence, NKCC1, and other isoforms, within the epithelium of the major osmoregulatory organs. Different (absorptive and secretory) functions have been attributed to this protein according to the tissue and salinity. The effects of short- (1-4 days), medium- (7-21 days) and long (6 months)-term freshwater (FW) adaptations were investigated, in comparison with seawater (SW)-maintained sea bass. In adult sea bass after...
Tipo: Text Palavras-chave: Quantitative expression; Immunofluorescence; Osmoregulation; Ontogeny; Dicentrarchus labrax; Na+/K+/2Cl( ) cotransporter.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-3531.pdf
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Physiological and biochemical changes of wild and cultivated juvenile pink shrimp Farfantepenaeus duorarum (crustacea: penaeidae) during molt cycle ArchiMer
Luis Bonilla-gomez, Jose; Chiappa-carrara, Xavier; Galindo, Carmen; Jeronimo, Gilberto; Cuzon, Gerard; Gaxiola, Gabriela.
Changes in metabolite levels in hemolymph and hepatopancreas were used as indicators of physiological status of juvenile wild and cultivated Farfantepenaeas. duorarum (Burkenroad, 1939), during hyper-osmoregulatory conditions (salinity 22 ppt, 726 mOsm/kg). We analyzed the relationship between biochemical changes of wild and cultivated shrimp at different molt stages by measurement of osmotic capacity, hemocyanin, acylglycerides, cholesterol, glucose, glycogen and total protein. Biochemical analyses of hemolymph (blood) and hepatopancreas showed a decreasing trend in stages closer to molt and an increasing trend before intermolt. Osmotic capacity and blood proteins indicated a significant effect of the molt stage. Stages A. D1' and D''' showed the greatest...
Tipo: Text Palavras-chave: Biochemical analysis; F. duorarum; Molt stages; Osmoregulation.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19936/17773.pdf
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IMPROVING THE TOLERANCE OF Vicia faba AGAINST ENVIRONMENTAL SALINITY RESULTED FROM THE IRRIGATION WITH SEA WATER BY USING KNO3 AND (NH4)2SO4 AS CHEMICAL OSMOREGULATORS Acta biol.Colomb.
MOHAMED,KAMEL.
The familiar solutes, Na+, K+, Ca2+, Mg2+, Cl-, PO4(3-), SO4(2-), soluble carbohydrates, amino acids and soluble proteins, which play a role in osmotic adjustment were estimated to investigate the role of potassium nitrate and ammonium sulphate as osmoregulators and their effects on the solutes composition. Vicia faba L. was cultivated and irrigated with 5, 10, 15 and 20 % (v:v) sea water. The plants were divided to three groups. The first was irrigated with sea water only. The second was treated with 5 mM KNO3 while the third was treated with 5mM (NH4)2SO4. The plants were left to grow until flowering stage. The results indicated that the non treated group increased the soluble carbohydrates in the roots to avoid the influx of sodium. The treatment with...
Tipo: Journal article Palavras-chave: Osmoregulation; Irrigation; Salinity; Sea water; Vicia faba.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000200006
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Response of the blue shrimp Litopenaeus stylirostris to temperature decrease and hypoxia in relation to molt stage ArchiMer
Mugnier, Chantal; Soyez, Claude.
The effect of different levels of dissolved oxygen (DO) and a decreased temperature combined with severe hypoxia were studied in the blue shrimp Litopenaeus stylirostris in relation to molt stage. In the first experiment, shrimps were submitted to DO concentrations of 5 to 1 mg l(-1). Osmoregulatory capacity (OC) was measured in shrimps at molt stage C after 6, 24, or 48 h of exposure. No mortality was recorded but a significant negative effect of DO concentration on hypo-OC was observed for DO concentrations below 3 mg l(-1). Osmoregulation improved after 48 h exposure, compared to 6 and 24 h exposure. In a second experiment, temperature was decreased from 28 to 22 degreesC in 24 h, before a severe hypoxia of 0.5+/-0.1 mg O-2 l(-1) was applied....
Tipo: Text Palavras-chave: Temperature; Stress; Penaeid shrimp; Osmoregulation; Molt stage; Litopenaeus stylirostris; Hypoxia.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-372.pdf
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Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase ArchiMer
Pham, Dominique; Charmantier, Guy; Boulo, Viviane; Wabete, Nelly; Ansquer, Dominique; Dauga, Clement; Grousset, Evelyse; Labreuche, Yannick; Charmantier-daures, Mireille.
The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Na+/K+-ATPase; RNA interference.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00311/42176/41477.pdf
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Salinity tolerance, ontogeny of osmoregulation and zootechnical improvement in the larval rearing of the Caledonian Blue Shrimp, Litopenaeus stylirostris (Decapoda, Penaeidae) ArchiMer
Pham, Dominique; Charmantier, Guy; Wabete, Nelly; Boulo, Viviane; Broutoi, Francis; Mailliez, Jean-rene; Peignon, Jean-marie; Charmantier-daures, Mireille.
The ontogeny of osmoregulationwas investigated in Litopenaeus stylirostris by studying salinity tolerance and osmoregulatory capacity. Shrimp at different larval and postlarval stages were exposed to various salinities and survival was monitored for 24 h. Survival rates exceeded 80% at salinity over 25 ppt (750 mOsm.kg(-1)) at all the stages. At salinities below to 25 ppt, salinity tolerance was higher in nauplii and zoeae than in mysis larvae. Postlarval stages were able to withstand lower salinities, e.g. 6.0 ppt (176 mOsm.kg(-1)) at PL9 stage, but they were more sensitive than larvae to salinities over 35 ppt (1035 mOsm.kg(-1)). Zoea and mysis larvae slightly hyper-regulated at all tested salinities. After metamorphosis, postlarvae progressively...
Tipo: Text Palavras-chave: Penaeid; Litopenaeus stylirostris; Ontogeny; Osmoregulation; Growth.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21843/20058.pdf
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Metabolic substrates are not mobilized from the osmoregulatory organs (gills and kidney) of the estuarine pufferfishes Sphoeroides greeleyi and S. testudineus upon short-term salinity reduction Neotropical Ichthyology
Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A..
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5‰ (for 6 h) were compared to those kept in 35‰. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009
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The effects of low salinity on lipid composition in the gills of grey mullet "Liza ramada" OceanDocs
Rabeh, I.; Telahigue, K.; Chetoui, I.; Masmoudi, W.; El Cafsi, M..
Les alevins de Liza ramada adaptés à l’eau de mer (EM, 35ppt) ont subi au laboratoire un stress halin par transfert direct de l’eau de mer à l’eau douce (ED, 0,5 ppt) pendant 24 heures. Les aspects hématologiques ainsi que la composition lipidique du tissu branchial des alevins ont été suivis. Le transfert à la basse salinité s’est accompagné d’une diminution significative de l’osmolalité du plasma, du taux d’hématocrite et du nombre des globules rouges. Le suivi de l’évolution des lipides des branchies des alevins en fonction de la salinité du milieu, montre une augmentation des acides gras monoinsaturés, particulièrement l’acide oleique C18 :1(n-9) à la basse salinité. Une réduction des pourcentages des acides gras polyinsaturés de la famille...
Tipo: Journal Contribution Palavras-chave: Osmoregulation; Mullet fisheries; Osmotic adaptations.
Ano: 2010 URL: http://hdl.handle.net/1834/4701
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