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ChlamyCyc - a comprehensive database and web-portal centered on _Chlamydomonas reinhardtii_ Nature Precedings
Jan-Ole Christian; Patrick May; Stefan Kempa; Dirk Walther.
*Background* - The unicellular green alga _Chlamydomonas reinhardtii_ is an important eukaryotic model organism for the study of photosynthesis and growth, as well as flagella development and other cellular processes. In the era of high-throughput technologies there is an imperative need to integrate large-scale data sets from high-throughput experimental techniques using computational methods and database resources to provide comprehensive information about the whole cellular system of a single organism.
*Results* - In the framework of the German Systems Biology initiative GoFORSYS a pathway/genome database and web-portal for _Chlamydomonas reinhardtii_ (ChlamyCyc) was established, which currently features about 270 metabolic pathways...
Tipo: Poster Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3108/version/1
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Chloroplast and Cell Imaging at Submicron Resolution by Two-Photon Excitation Nature Precedings
I C. Baianu.
Novel, two-photon NIR excitation fluorescence correlation microspectroscopy tests and preliminary results were presented in this article with submicron resolution for concentrated suspensions of functional cells and chloroplast membranes. Related developments of these technique involve applications of Fluorescence Cross-Correlation Spectroscopy (FCCS) detection to monitoring: 
DNA- telomerase interactions, DNA hybridization kinetics, ligand-receptor interactions, and HIV-HBV testing. 

Tipo: Manuscript Palavras-chave: Biotechnology; Cancer; Chemistry; Genetics & Genomics; Immunology; Molecular Cell Biology; Bioinformatics; Plant Biology; Data Standards.
Ano: 2012 URL: http://precedings.nature.com/documents/7116/version/1
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Chromium uptake by Fenugreek Nature Precedings
Xanthate D S; Murthy Ch V R; Rajan S C S; Sekhar D M R.
Fenugreek (Trigonella foenum- graecum) is both herb (leaves) and a spice (seed) belonging to the family Fabaceae. Fenugreek leaves and seeds are used in the cuisine of India. Fenugreek also has medicinal value. Fenugreek seeds are known to reduce serum glucose and improve glucose tolerance and hence are prescribed to diabetic patients. In the recent past supplemental Chromium is being prescribed to diabetic patients to activate (increased- insulin binding, insulin receptor number, insulin receptor phosphorylation) insulin. Plants can uptake substantial quantities of toxic metals from contaminated soils if these soils are well ameliorated. 

It is then probable that the medicinal efficacy of Fenugreek in the case of...
Tipo: Manuscript Palavras-chave: Pharmacology; Plant Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/6762/version/1
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CO2 Enhancement of Forest Productivity Constrained by Limited Nitrogen Availability Nature Precedings
Richard J. Norby; Jeffrey M. Warren; Colleen M. Iversen; Charles T. Garten; Belinda E. Medlyn; Ross E. McMurtrie.
Stimulation of terrestrial productivity by rising CO~2~ concentration is projected to reduce the airborne fraction of anthropogenic CO~2~ emissions; coupled climate-carbon (C) cycle models, including those used in the IPCC Fourth Assessment Report (AR4), are sensitive to this negative feedback on atmospheric CO~2~^1^. The representation of the so-called CO~2~ fertilization effect in the 11 models used in AR4 and subsequent models^2,3^ was broadly consistent with experimental evidence from four free-air CO~2~ enrichment (FACE) experiments, which indicated that net primary productivity (NPP) of forests was increased by 23 +/- 2% in response to atmospheric CO~2~ enrichment to 550 ppm^4^. Substantial uncertainty remains, however, because of the expectation...
Tipo: Manuscript Palavras-chave: Earth & Environment; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3747/version/1
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Codonopsis pilosula twines either to the left or to the right Nature Precedings
Gonghua Lin; Fang Zhao; Eviatar Nevo; Tongzuo Zhang; Jianping Su.
We report the twining handedness of Codonopsis pilosula, which has either a left- or right-handed helix among different plants, among different tillers within a single plant, and among different branches within a single tiller. The handedness was randomly distributed among different plants, among the tillers within the same plants, but not among the branches within the same tillers. Moreover, the handedness of the stems can be strongly influenced by external forces, i.e. the compulsory left and right forming inclined to produce more left- and right-handed twining stems, respectively, and the reversing could make a left-handed stem to be right-handed and vice versa. We also discuss the probable mechanisms these curious cases happen.
Tipo: Manuscript Palavras-chave: Ecology; Plant Biology; Evolutionary Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7026/version/1
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Comment on Lobell et al "Climate Trends and Global Crop Production Since 1980" Nature Precedings
Mikhail A. Semenov; Peter R. Shewry.
Lobel et al (ScienceXpress) analysed changes in global yields which can be attributed to climate change trend, and reported a 5.5% decline in global wheat production since 1980. We have failed to confirm these results using a process-based crop model, which showed no decline in wheat yield since the 1950s at two European sites. The Lobel et al conclusions are not valid due to the limitations of the regression analysis used.
Tipo: Manuscript Palavras-chave: Earth & Environment; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6289/version/1
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Comparative Litter Quality and Recalcitrance Among Native Grasses and the Invasive, Non-indigenous KR Bluestem (_Bothriochloa ischaemum_) Nature Precedings
Amanda Benbow; Kelly G. Lyons.
KR Bluestem (_Bothriochloa ischaemum_) is a non-indigenous, invasive, C4 grass that was introduced throughout the Midwestern and Southwestern U.S. short- to midgrass prairies with the aim of improving degraded rangelands. The aggressive nature of KR bluestem has led to dramatic alterations of natural and managed ecosystems. Comparative studies of decomposition often show that non-indigenous, invasive plant species have higher rates of nutrient cycling than indigenous species; however, KR bluestem appears to deviate from this trend. Large amounts of litter are observed in KR-dominated grasslands as compared to intact native grasslands, suggesting that the species has a relatively lower decomposition rate and may slow nutrient cycling in these systems....
Tipo: Presentation Palavras-chave: Ecology; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3614/version/1
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Control of konzo in the Democratic Republic of Congo Nature Precedings
J. Howard Bradbury; Chretienne Mandombi; D Nahimana; J. P. Banea; Ian C. Denton; N Kuwa.
Konzo is an upper motor neuron disease that causes irreversible paralysis of the legs mainly in children and young women^1,2^, due to consumption of large amounts of cyanogens from poorly processed cassava, the staple food of tropical Africa^3^. Konzo occurs in the Democratic Republic of Congo (DRC),Mozambique, Tanzania, Cameroon, Central African Republic and Angola. In March 2010 the wetting method, which removes cyanogens from cassava flour^4,5,6^, was taught to and used by the mothers of Kay Kalenge village. This reduced the total cyanide content of cassava flour to the FAO/WHO limit of 10ppm^7^. Cyanogen intake of school children, monitored by urinary thiocyanate analyses, decreased from mean values of 332 to 130 μmole/L. The percentage of...
Tipo: Manuscript Palavras-chave: Chemistry; Neuroscience; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6520/version/1
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Controlling sap-sucking insect pests with recombinant endophytes expressing plant lectin Nature Precedings
Xiuyun Zhao; Gaofu Qi; Xianfang Zhang; Nan Lan; Xinrong Ma.
We developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins. Fungal endophyte of Chaetomium globosum YY-11 with anti-fungi activities was isolated from rape seedlings, and bacterial endophytes of SJ-10 (Enterobacter sp.) and WB (Bacillus subtilis) were isolated from rice seedlings. Pinellia ternate agglutinin gene was cloned into SJ-10 and WB for expression by a shuttle vector, and YY-11 was mediated by Agrobacterium tumefaciens. Positive transformants were evaluated using PCR and Western blot assay. Recombinant endophytes colonized most of crops, and resistance of rice seedlings, which were inoculated with the recombinant endophytic bacteria, to white backed planthoppers was dramatically enhanced by decreasing...
Tipo: Manuscript Palavras-chave: Biotechnology; Microbiology; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4862/version/1
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Copper and other heavy metals in specialized crops and their effects on soil coenosis Nature Precedings
Anna Steindl; Thomas Strumpf; Frank Riepert.
The background of the project are claims of the EU regulation to generate reliable data considering the extent of copper contamination in soils of specialized crops in order to decide about copper restrictions in organic farming.

2522 single soil samples were collected on 85 vineyard and 13 hop areas with different management histories. Samples were taken from sites presently under cultivation, formerly cultivated, and sites without anthropogenic copper contamination as control, indicating natural background values. In addition to Aqua Regia extraction for determining total copper contents, available plant copper content was analyzed in NH~4~NO~3~ extraction. Soil parameters such as pH-value, C/N ratio, soil type etc....
Tipo: Presentation Palavras-chave: Chemistry; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5737/version/1
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Copper induced peroxidase, CCPP form Caribbean copper plant Nature Precedings
Reetesh Kumar.
Plant peroxidase exhibit broad substrate specificity and is active in a wide range of pH and temperature, and in the presence of organic compounds as well as other additives. The peroxidase is known to be involved in many physiological and regulatory processes in plants. This ppt presents an introduction on plant peroxidase and their applications. A purification protocol has been developed and the purified protein, named as CCPP is biochemically and physiologically characterized. The physiochemical properties such as molecular weight, isoelectric point have been experimentally determined. The enzymatic efficiency has been evaluated through temperature optimum, pH optimum, Km and other enzymatic properties along with spectroscopic parameters like circular...
Tipo: Presentation Palavras-chave: Biotechnology; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/6410/version/1
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Cost effective mass multiplication of Gymnema sylvestre in hydroponic system Nature Precedings
Raju Karthic; Sundaram Seshadri.
Present experiment was designed with an objective to develop a cost efficient mass multiplication method for Gymnema sylvestre using hydroponic system. A plastic tub, with polyethylene cover, containing 1/10 strength of MS salts supplemented with Indole butyric acid (IBA) at different concentrations (0.5, 1.0, 2.5 mg/L) was studied. Medium containing 0.5mg/L of IBA produced highest rooting (66%) with 96 % survival. This protocol will serve as an alternative to the existing in vitro and clonal multiplication protocols.
Tipo: Manuscript Palavras-chave: Biotechnology; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3770/version/1
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Crinumin, a novel substitute of chymotrypsin, may control pancreatic insufficiency and inflammation: Purification and characterization Nature Precedings
Kunwar Awaneesh Singh; Chandan Singh; G.R.K. Rao; M.V. Jagannatham.
Crinumin, a glycosylated serine protease with chymotrypsin like catalytic specificity was purified from a medicinally important plant _Crinum asiaticum_ of family _Amaryllidaceae_. Ethno-botanical information and Pharmacological studies confirming about the presence of active bio- molecules in the _Crinum_ played crucial role in injury, inflamed joint, local pain and arthritis. Biomedical research suggests chymotrypsin was not only used as digestive aid but also helpful in the above disease. These findings support towards the crinumin may be the active bio-molecule. Crinumin shows activity over a wide range of pH (4.5-11.5 and optimum at 8.5), temperature (75 °C and optimum at 70 °C) and is also functional against chaotrophs, organic...
Tipo: Poster Palavras-chave: Pharmacology; Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4339/version/1
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Critical studies on systematics of Murdannia lanuginosa (Wall. EX C. B. Cl) Brueck. (Commelinaceae) Nature Precedings
M D. Nandikar; R V. Gurav.
_Murdannia lanuginosa_ (C. B. Cl.) Brucek. (Commelinaceae), an endemic plant to Western Ghats of Peninsular India. It is categorized as regionally endangered taxon owing to its restricted habitat and low population status in ateritic plateaus of Maharashtra and Karnataka. It is observed in higher abundance in lateritic plateaus and hilly slopes at an altitude range of 900 to 1600 m. _M. lanuginosa_ found on open grassy plateaus along with herbs like _M. simplex_, _M. versicolor_, _Ischaemum impressum_, _Glyphochloa forficulata_, _Paspalum canarae_, _Smithia bigemina_, _S. purpurea_, _Rotala densiflora_, _Pogostemon deccanensis_, etc. Extent of occurrence of _M. lanuginosa_ estimated to be 4,000 sq. km and found only at few locations. This paper presents...
Tipo: Poster Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4619/version/2
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Critical studies on systematics of Murdannia lanuginosa (Wall. EX C. B. Cl) Brueck. (Commelinaceae) Nature Precedings
M D. Nandikar; R V. Gurav.
_Murdannia lanuginosa_ (C. B. Cl.) Brucek. (Commelinaceae), an endemic plant to Western Ghats of Peninsular India. It is categorized as regionally endangered taxon owing to its restricted habitat and low population status in ateritic plateaus of Maharashtra and Karnataka. It is observed in higher abundance in lateritic plateaus and hilly slopes at an altitude range of 900 to 1600 m. _M. lanuginosa_ found on open grassy plateaus along with herbs like _M. simplex_, _M. versicolor_, _Ischaemum impressum_, _Glyphochloa forficulata_, _Paspalum canarae_, _Smithia bigemina_, _S. purpurea_, _Rotala densiflora_, _Pogostemon deccanensis_, etc. Extent of occurrence of _M. lanuginosa_ estimated to be 4,000 sq. km and found only at few locations. This paper presents...
Tipo: Poster Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4619/version/1
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Crossing Lilium Orientals of different ploidy creates Fusarium-resistant hybrid Nature Precedings
Zheng Sixiang; Wu Hongzhi; Zhao Yan; Liu Feihu.
Oriental hybrid lily is of great commercial value, but it is susceptible to Fusarium disease that causes a significant loss to the production. A diploid Oriental hybrid resistant to Fusarium, Cai-74, was diploidized from triploid obtained from the offspring of tetraploid (from ‘Star Fighter’) and diploid (‘Con Amore’, ‘Acapulco’) by screening the hybrids of different cross combinations following inoculating Fusarium oxysporum to the tissue cultured plantlets in a greenhouse. By analyzing saponins content in bulbs of a number of lily genotypes with a known Fusarium resistance, it was found that the mutant Cai-74 had a much higher content of saponin than its parents. Highly resistant wild _L....
Tipo: Manuscript Palavras-chave: Plant Biology.
Ano: 2010 URL: http://precedings.nature.com/documents/4830/version/1
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Cu2+ uptake by Chlorococcum hemicolum - A Xeric Chlorophycean Alga Nature Precedings
Mr. Harish; S. Sundaramoorthy; Devendra Kumar; S.G. Vaijapurkar.
Bioremediation of copper by xeric chlorophycean bioremediator, Chlorococcum hemicolum was investigated. The growth rates at various concentrations of Cu2+ were assessed in terms of protein level and 8 mg L-1 (37.67 % level in growth kinetics) is the tolerance limit. Absorption/adsorption kinetics was estimated after 240 hrs of Cu2+ treatments. Absorptions were higher than adsorption with maximum accumulation factor (AF) of 1.40. The Cu2+ concentration and absorption were linearly related (r = 0.99; p>0.01). Other biochemical parameters like total sugar, chlorophyll and carotenoids were also quantified to correlate the state of metabolism and these exhibited reduction due to heavy metal stress.
Tipo: Manuscript Palavras-chave: Ecology; Earth & Environment; Plant Biology.
Ano: 2011 URL: http://precedings.nature.com/documents/5657/version/1
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Current status of herbal and their future perspectives Nature Precedings
Ramar Perumal R. Samy; Ponnampalam Gopalakrishnakone.
Traditional medicine is the synthesis of therapeutic experience of generations of practicing physicians of indigenous systems of medicine. Throughout the history of mankind, many infectious diseases have been treated with herbals. The traditional medicine is increasingly solicited through the tradipractitioners and herbalists in the treatment of infectious diseases. Among the remedies used, plant drugs constitute an important part. A number of scientific investigations have highlighted the importance and the contribution of many plant families i.e. Asteraceae, Liliaceae, Apocynaceae, Solanaceae, Caesalpinaceae, Rutaceae, Piperaceae, Sapotaceae used as medicinal plants. Medicinal plants play a vital role for the development of new drugs (export and import...
Tipo: Manuscript Palavras-chave: Pharmacology; Plant Biology.
Ano: 2007 URL: http://precedings.nature.com/documents/1176/version/1
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Data Mining of the Coffee Rust Genome Nature Precedings
David Octavio Botero-Rozo; William Giraldo; Alvaro Gaitan; Marco Cristancho; Diego M. Riaño-Pachon; Silvia Restrepo.
The genomes of nine isolates of _Hemileia vastatrix_, the causal agent of coffee leaf rust were sequenced by Illumina and 454. Quality control, cleaning and _de novo_ assemblies of data were performed. Since isolates were obtained from the field and it is not possible to produce axenic cultures of _H. vastatrix_, MEGAN software was used to evaluate contamination levels and to select contigs with fungal similarities. Mitochondrial contigs were identified and annotated by comparing this assembly against the _Puccinia_ genome. Furthermore, two transcriptomes from isolates of _H. vastatrix_ were assembled to complement the genomic data.
Tipo: Poster Palavras-chave: Genetics & Genomics; Bioinformatics; Plant Biology.
Ano: 2012 URL: http://precedings.nature.com/documents/7034/version/1
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Decadal-scale changes in seagrass coverage on the Mississippi barrier islands, northern Gulf of Mexico Nature Precedings
Gregory A. Carter; G. Alan Criss; Gabriel A. Blossom; Patrick D. Biber.
Seagrasses provide habitat for a wide variety of aquatic species, and reduce coastal erosion via sediment stabilization. The areal coverage of seagrass beds on the Mississippi barrier islands appears to have declined substantially since the 1960s. However, difficulties in sampling benthic vegetation, differences among previous studies in survey methods, and a lack of data on year-to-year variation in coverage have impeded quantitative study of this decline. Thus, the goal of the present study was to utilize historical remote sensing data to quantitatively determine changes in seagrass (predominantly Halodule wrightii Ascherson) coverage on the Mississippi barrier islands. Analyses were limited to imagery acquired during late summer through autumn, when...
Tipo: Poster Palavras-chave: Ecology; Earth & Environment; Plant Biology.
Ano: 2009 URL: http://precedings.nature.com/documents/3669/version/1
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