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Registros recuperados: 9
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Comportements cellulaires et régulation génétique au cours des réactions d'immunité innée chez la moule Mytilus galloprovincialis ArchiMer
Li, Hui.
The mytiliculture is one of the most important aquaculture in the world, however, little is known on the mussel immune system, which plays a critical role in case of infectious diseases. Like all the other invertebrates, our model, the Mediterranean mussel Mytilus galloprovincialis, possesses only an innate immune system considered to be primitive and non-specific. In the present study, our results showed that the mussel immune system (i) is capable of responding differently to a variety of stimulations, suggesting a specific recognition process; (ii) reacts differently according to the geographic origin of the animals, showing an adaptation to their own environment; (iii) is influenced by the season, which explains the seasonal mortalities; (iv) presents...
Tipo: Text Palavras-chave: Mytilus; Haemocyte; Antimicrobial peptides; Polymorphism; Genetic regulation; Innate immunity; Mytilus; Hémocyte; Peptides antimicrobiens; Polymorphisme; Régulation génétique; Immunité innée.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-6241.pdf
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Etude des effets de la sélection sur les gènes de l'amylase chez une population d'huître creuse Crassostrea gigas en fonction des conditions trophiques précoces ArchiMer
Sauvage, Christopher.
In natural population of the Pacific cupped oyster, there are differences between allelic and genotypic frequencies for the A and B amylase genes. Set up a synthetical population of C. gigas, in which whole alleles and genotypes are better represented than in wild population, was done. An investigation links between trophic conditions applied during larvae developpment (in nursery), allelic frequencies for both amylase genes, oyster's individual waight and by the selectivity of summer mortalities during their exposition on the estran has been done. As a result, following facts have been observed : - On one side, an influence of the trophic condition applied during the larvae development on genotypic frequencies of the gene B of the amylase - On the other...
Tipo: Text Palavras-chave: Crassostrea gigas; Amylase; Polymorphisme; Condition trophique; Crassostre gigas; Amylase; Polymorphim; Trophic condition.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00371/48185/48300.pdf
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Herpèsvirus infectant les bivalves marins : détection, génome et transmission ArchiMer
Arzul, Isabelle.
Since 1991, high mortality rates of larvae and juveniles of different bivalve mollusc species associated with herpesvirus infections have been reported in different countries. The aim of this study was to determine if more than one helpesvirus species was responsible for these infections or just one species exhibiting polymorphism. The investigations concentrated on the analysis of the virus genome, studies of virus transmission in different mollusc species and on the potential role of bivalve adults in the occurrence of infections. The analysis of various target sequences within the virus genome present in infected larvae samples demonstrated the presence of polymorphism. More notably a variant of the virus was identified and the variation appears...
Tipo: Text Palavras-chave: Herpèsvirus; Bivalves; Transmission; Polymorphisme; OsHV-1; Diagnostic; Herpesviruses; Bivalvia; Transmission; Polymorphism; OsHV-1; Diagnostic.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00105/21675/19253.pdf
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Etude du polymorphisme des gènes de l'amylase chez Crassostrea gigas (Thunberg, 1793). Relation avec les paramètres physiologiques de l'assimilation ArchiMer
Degremont, Lionel.
L'analyse du polymorphisme des gènes de J'amylase chez Crassostrea gigas (Thunberg, 1793) a été établi sur deux générations dans le cadre du programme européen GENEPHYS (génétique et physiologie chez C.gigas). Après une amplification de l'ADN par PCR à partir de deux couples d'amorces qui sont spécifiques à chacun des deux gènes de l'amylase, une digestion par EcoRI de l'ADN amplifié suivie d'une électrophorése en gel d'agarose sont effectuées. Cette technique (RFLP) permet de déterminer le polymorphisme génétique des huîtres creuses. Une troisième génération a été réalisée afin d'obtenir quatre familles et d'en analyser le génotype. Ces familles ont été tout d'abord placées pendant cinq mois dans un milieu riche en nourriture, puis ensuite, chaque famille...
Tipo: Text Palavras-chave: Crassostrea gigas; Amylase; Polymorphisme; Croissance; Efficacité d'assimilation.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00439/55109/56561.pdf
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Les gènes de l'amylase chez l'huître creuse Crassostrea gigas : transmission et mise en évidence des pressions sélectives ArchiMer
Rambelo-ramanitra, Seheno.
Abnormal massive mortalities of oysters Crassostrea gigas during the estival period are a phenonmenon described in the literature since forty years. A certain number of studies show that it could exist a genectic base with these mortalities. In front of the economic interest that the oyster production in France represents, IFREMER set up program MOREST, gathering the fiels of physiology, pathology and the genetics. This study in interested in rare genotypes, determined by PCR-RFLP, presenting three bands for one or two A and B amylase genes, met in the families MOREST and the polymorphic population for amylase genes, made up starting from crossing of the hierarchical type. It aims at making a follow-up of the segregation of the alleles at the progeny, as...
Tipo: Text Palavras-chave: Crassostrea gigas; Extraction d'ADN; Amylase; Polymorphisme; Génotypes; Crassostrea gigas; DNA Extraction; Amylase; Polymorphism; Genotypes.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00371/48230/48364.pdf
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Aide pour l'observation d'Ansates sur des laminaires ArchiMer
Huet, Jerome.
Photos of young and intermediate-age Ansates pellucida. Photos of old individuals. Photos of Polymorphism. Photos demonstrating the presence of some Ansates pellucida under the holdfast of Laminaria hyperborea and the presence of some Ansates pellucida on a stipe of Laminaria digitata.
Tipo: Text Palavras-chave: Polymorphisme; Laminaria hyperborea; Laminaria digitata; Ansates pellucida.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/rapport-6124.pdf
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Etude de la variabilité génétique de populations naturelles d'huîtres creuses Crassostrea gigas (Thunberg) du littoral Atlantique Français ArchiMer
Gras, Marie-paule; Gras, Paul.
The aductor muscle proteins of Japaneses oysters C. gigas collected from three different geographical wild stocks were studied by acrylamid gel electrophoreseis. The first results reveal a biochemical polymorphism of a protein fraction
Tipo: Text Palavras-chave: Crassostrea gigas; Oysters; Genetic; Polymorphism; Genetic population; Crassostrea gigas; Huîtres; Génétique; Polymorphisme; Populations naturelles.
Ano: 1982 URL: http://archimer.ifremer.fr/doc/1982/acte-2952.pdf
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Etude du polymorphisme des gènes de l'amylase chez Crassostrea gigas : relation avec les paramètres physiologiques de l'assimilation et croissance ArchiMer
Degremont, Lionel; Moal, Jeanne; Daniel, Jean-yves; Boudry, Pierre; Van Vormhoudt, A.; Sellos, D.; Samain, Jean-francois.
In the scope of the European program GENEPHYS, which aims to establish the relationship between the genetic base and the physiological characters in relation to the growth in Crassostrea gigas, it has been demonstrated that the most explanatory factors for the growth variability are assimilation performance and aneuploidy. A model linking the assimilation performances and the digestive equipment has been set up and more than 70% of the variability can be explained by two digestive enzymes: laminarinase and amylase. A number of laboratories have decided to focus on the latter.
Tipo: Text Palavras-chave: Croissance; Génétique; Polymorphisme; Crassostrea gigas.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/acte-3269.pdf
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Application des techniques moléculaires de PCR-RFLP et de PCR-RFLP-SSCP dans l'étude de la différenciation génétique de deux huîtres creuses : Crassostrea gigas et Crassostrea angula ArchiMer
Dufourg, Cecile.
Les 2 taxons d'huîtres creuses C. angulata et C. gigas n'étant pas différenciables sur des critères morphologiques, écologiques ou physiologiques, l'étude de leur passé évolutif fait appel à l'utilisation de marqueurs génétiques. L'étude du polymprohisme des séquences mitochondriales codant pour la sous-unité I de la cytochrome oxydase par la technique de PCR-RFLP a été réalisée sur 197 individus appartenant à 5 populations d'origine géographique différente, de type Crassostrea angulata ou Crassostrea gigas. Elle a permis la construction d'un arbre représentant les relations phylogénétiques entre ces populations. Cet arbre a été comparé à celui établi à partir de l'analyse de 3 marqueurs microsatellites. Les résultats de ces 2 analyses sont similaires et...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea angulata; Crassostrea gigas; Génétique; Polymorphisme; ADN; SSCP; Biologie moléculaire.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00033/14415/11709.pdf
Registros recuperados: 9
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