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Production of thermostable invertases by Aspergillus caespitosus under submerged or solid state fermentation using agroindustrial residues as carbon source BJM
Alegre,Ana Cláudia Paiva; Polizeli,Maria de Lourdes Teixeira de Moraes; Terenzi,Héctor Francisco; Jorge,João Atílio; Guimarães,Luis Henrique Souza.
The filamentous fungus Aspergillus caespitosus was a good producer of intracellular and extracellular invertases under submerged (SbmF) or solid-state fermentation (SSF), using agroindustrial residues, such as wheat bran, as carbon source. The production of extracellular enzyme under SSF at 30ºC, for 72h, was enhanced using SR salt solution (1:1, w/v) to humidify the substrate. The extracellular activity under SSF using wheat bran was around 5.5-fold higher than that obtained in SbmF (Khanna medium) with the same carbon source. However, the production of enzyme with wheat bran plus oat meal was 2.2-fold higher than wheat bran isolated. The enzymatic production was affected by supplementation with nitrogen and phosphate sources. The addition of glucose in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus caespitosus; ²-D-fructofuranosidase; Invertase; Solid-state fermentation; Submerged fermentation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300025
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Protein enrichment of cactus pear (Opuntia ficus - indica Mill) using Saccharomyces cerevisiae in solid-state fermentation BABT
Araújo,Lúcia de Fátima; Medeiros,Ariosvaldo Nunes; Perazzo Neto,Américo; Oliveira,Líbia de Sousa Conrado; Silva,Flávio Luiz Honorato da.
The microbial protein bioconversion of cactus pear by yeast in solid medium was studied. Three cultivation variables used were: inoculum's concentrations (5, 10 and 15 %), substrate layer thickness (2, 4 and 6 cm) and temperature (30, 34 and 38 ºC). The rate of dry matter production and total protein were determined. Results obtained were variance analysis, gross energy and in vitro dry matter digestibility. The maximum protein amount achieved for the conditions studied in the present work was higher than 26 %, which was compatible or greater than those of conventional concentrates of protein supplements used for animal feed. The protein concentrate of cactus pear had a higher in vitro digestibility index (95.8 %) and did not show any changes in the gross...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protein enrichment; Solid-state fermentation; Cactus pear; Yeast.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400020
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Improvement of xylanase production by Cochliobolus sativus in solid state fermentation BJM
Bakri,Yasser; Jawhar,Mohammed; Arabi,Mohammed Imad Eddin.
The xylanase production by Cochliobolus sativus strain Cs6 was improved under solid state fermentation (SSF). High xylanase activity (1079 U/g) was obtained when wheat straw was used after 8 days of incubation. Combinations of sodium nitrate with peptone or yeast extract resulted in an increased xylanase production (1543 and 1483 U/g, respectively). The Cs6 strain grown in SSF in a simple medium, proved to be a promising microorganism for xylanase production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cochliobolus sativus; Xylanase; Solid-state fermentation.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300036
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Solid state fermentation of Bacillus thuringiensis tolworth as a toll for Spodoptera frugiperda control in maize. Repositório Alice
CAPALBO, D. M. F.; VALICENTI, F.; MORAES, I.O.; SOARES, C.M..
1996
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Entomopathogenic bacterium; Bioinsecticide; Fermentation process; Solid-state fermentation; Production; Biocontrol agent.
Ano: 1996 URL: http://www.alice.cnptia.embrapa.br/handle/doc/12564
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Assessment of the effects of intermittent mixing on solid-state fermentation for biomass-degrading enzymes production by different fungal strains. Repositório Alice
CASSARO, E. G.; FONSECA, R. F.; FARINAS, C. S..
bitstream/item/180868/1/Assessment-of-the-effects-of-intermittent-mixing-on-solid-state.pdf
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Solid-state fermentation; Aspergillus Niger; Aspergillus Oryzae; Trichoderma reesei; Agitation; Cellulases; Sugarcane bagasse; Xylanases.
Ano: 2015 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1035699
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Lipase production by solid-state fermentation in fixed-bed bioreactors BABT
Cavalcanti,Elisa d'Avila Costa; Gutarra,Melissa Limoeiro Estrada; Freire,Denise Maria Guimarães; Castilho,Leda dos Reis; Sant'Anna Júnior,Geraldo Lippel.
In the present work, packed bed bioreactors were employed with the aim of increasing productivity and scaling up of lipase production using Penicillium simplicissimum in solid-state fermentation. The influence of temperature and air flow rate on enzyme production was evaluated employing statistical experimental design, and an empirical model was adjusted to the experimental data. It was shown that higher lipase activities could be achieved at lower temperatures and higher air flow rates. The maximum lipase activity (26.4 U/g) was obtained at the temperature of 27°C and air flow rate of 0.8 L/min.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid-state fermentation; Fixed-bed bioreactors; Lipase; Penicillium simplicissimum.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400010
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Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation BABT
Costa,Jorge Alberto Vieira; Colla,Eliane; Magagnin,Glênio; Santos,Lucielen Oliveria dos; Vendruscolo,Mauricio; Bertolin,Telma Elita.
Amyloglucosidase (AMG) and exo-polygalacturonase (exo-PG) were simultaneously produced by two different strains of Aspergillus niger in solid-state fermentation (SSF) using defatted rice-bran as substrate. The effect of Aspergillus niger strain (t0005/007-2 and/or CCT 3312), inoculum type (spore suspension or fermented bran) and addition of inducers (pectin and/or starch) to the culture media was studied using a 3² x 2¹ factorial experimental design. The production of AMG and exo-PG was significantly affected by fungal strain and inoculum type but inducers had no effect. The maximum yields obtained were 1310 U/g dm for AMG using a spore suspension of A. niger CCT 3312 and 50.2 U/g dm for exo-PG production, using A. niger t0005/007-2 and fermented bran as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amyloglucosidase; Exo-polygalacturonase; Rice-bran; Solid-state fermentation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000500003
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Comparison of Aspergillus niger spore production on Potato Dextrose Agar (PDA) and crushed corncob medium. Repositório Alice
FARIAS, V. L. de; MONTEIRO, K. X.; RODRIGUES, S.; FERNANDES, F. A. N.; PINTO, G. A. S..
2010
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Aspergillus niger; Corncob; Low-cost medium; Solid-state fermentation.
Ano: 2010 URL: http://www.alice.cnptia.embrapa.br/handle/doc/877901
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Simultaneous allergen inactivation and detoxification of castor bean cake by treatment with calcium compounds BJMBR
Fernandes,K.V.; Deus-de-Oliveira,N.; Godoy,M.G.; Guimarães,Z.A.S.; Nascimento,V.V.; Melo,E.J.T. de; Freire,D.M.G.; Dansa-Petretski,M.; Machado,O.L.T..
Ricinus communis L. is of great economic importance due to the oil extracted from its seeds. Castor oil has been used for pharmaceutical and industrial applications, as a lubricant or coating agent, as a component of plastic products, as a fungicide or in the synthesis of biodiesel fuels. After oil extraction, a castor cake with a large amount of protein is obtained. However, this by-product cannot be used as animal feed due to the presence of toxic (ricin) and allergenic (2S albumin) proteins. Here, we propose two processes for detoxification and allergen inactivation of the castor cake. In addition, we establish a biological test to detect ricin and validate these detoxification processes. In this test, Vero cells were treated with ricin, and cell death...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ricinus communis; 2S albumin; Ricin; Biodiesel fuel; Solid-state fermentation; Vero cell.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001100002
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Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation BJM
Freitas,Paula Mendes de; Martin,Natalia; Silva,Dênis; Silva,Roberto da; Gomes,Eleni.
Polygalacturonases production by newly isolated Monascus sp N8 and Aspergillus sp N12 strains was carried out in solid-state fermentation using mixtures of wheat bran, sugar cane bagasse and orange bagasse as carbon sources. The maximal activity values of exo-polygalacturonases (exo-Pg) from Monascus sp and Aspergillus sp were obtained using wheat bran/sugar cane bagasse/orange bagasse mixture (6.6 U/mL) and wheat bran/orange bagasse mixture (10 U/mL), respectively. Enzyme production by both strains was higher at 45ºC after 72 h and 1.6 U/mL at 50ºC after 120 h. Endo-polygalacturonase (endo-Pg) production was higher in wheat bran/orange bagasse mixture and was not affected by temperature of incubation for both fungi. Endo-Pg production by Monascus was 1.8...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monascus sp; Aspergillus sp; Thermophilic; Polygalacturonase; Solid-state fermentation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300019
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Partial purification and properties of cellulase-free alkaline xylanase produced by Rhizopus stolonifer in solid-state fermentation BABT
Goulart,Antonio José; Carmona,Eleonora Cano; Monti,Rubens.
Rhizopus stolonifer was cultivated in wheat bran to produce a cellulase-free alkaline xylanase. The purified enzyme obtained after molecular exclusion chromatography in Sephacryl S-200 HR showed optimum temperature as 45º C and hydrolysis pHs optima as pH 6.0 and 9.0. Xylanase presented higher Vmax at pH 9.0 (0.87 µmol/mg protein) than at pH 6.0 and minor Km at pH 6.0 (7.42 mg/mL) than at pH 9.0.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline xylanase; Rhizopus stolonifer; Solid-state fermentation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300001
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Production and characterization of endo-polygalacturonase from Aspergillus niger in solid-state fermentation in double-surface bioreactor BABT
Hendges,Diogo Henrique; Montanari,Queli; Malvessi,Eloane; Silveira,Mauricio Moura da.
Endo-polygalacturonase (endo-PG) production by Aspergillus niger T0005/007-2 in solid medium with 170 mm of height was evaluated in a cylindrical double surface bioreactor in 96-h experiments. Cell concentration close to 92 mg.g -¹ dm (mg per g of dry medium) in the standard condition (static) was achieved, whereas in tests under forced aeration of 1.4 and 2.8 L.min-1. Kg-1 mm (L of air per minute per Kg of moist medium) and with the central shaft fungal biomass attained approximately 100 mg.g-1 dm. Superior endo-PG activity was obtained with the central-shaft system, 78 U.g-1 dm (units per g of dry medium). Forced aeration and pressure pulse showed no positive effect on the production of endo-PG, 45 U.g-1 dm and 28 U.g-1 dm, respectively. None of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Double-surface bioreactor; Endo-polygalacturonase; Solid-state fermentation; Enzyme stability.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200005
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Novel process for the simultaneous extraction and degumming of banana fibers under solid-state cultivation BJM
Jacob,Nicemol; Prema,Parukuttyamma.
Various process parameters for the production of polygalacturonase by Streptomyces lydicus under solid-state fermentation were optimized. The optimum particle size of wheat bran for polygalacturonase production was in the range of 500-1000 µm. Initial moisture content of 70% was found to be the optimum for enzyme production. The most suitable inoculum size was 1.25 x 10(5) CFU/mL and the optimum incubation temperature was 30ºC. Addition of carbon sources resulted in 37% increase in enzyme yield (425 U/g), whereas no significant enhancement was obtained on nitrogen supplementation. Maximum enzyme yield was recorded at 72 h. When compared to the initial production medium (108.5 U/g), the enzyme yield was 3.9 fold after optimization. Solid-state fermentation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Banana fiber degumming; Solid-state fermentation; Polygalacturonase; Streptomyces lydicus; Wheat bran.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100025
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Immunomodulation Properties of Solid-State Fermented Laetiporussulphureus Ethanol Extracts in Chicken Peripheral Blood Monocytes In Vitro Rev. Bras. Ciênc. Avic.
Lin,WC; Lee,MT; Lin,LJ; Chang,SC; Lee,TT.
ABSTRACT Laetiporus sp. is recognized as a fungal species traditionally used for medicinal purposes. This study investigated the in-vitro effects of solid-state fermented Laetiporussulphureus ethanol extracts (LSE) for their immunomodulatory potential. Bioactive levels detected in the LSE on different days throughout the fermentation period revealed that the 12th day was the most efficient, with 7.19 ± 0.66 GAE/g DM crude phenolic content, 2.71 ± 0.03 UAE/g DM crude triterpenoid content, 12.93 ± 0.88 GCE/g DM crude polysaccharides, and 96.44 ± 0.2 mg/g DM ergosterol content. In-vitroLSE tests on chPBMC showed no cytotoxicity within a range of 0.05-1 mg/mL, but LPS-inhibited cell viability was improved, as well as LPS-induced nitric oxide (NO) production...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laetiporussulphureus; In vitro; Immunomodulatory; Solid-state fermentation.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400316
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Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus BJM
Machado,Kátia Maria Gomes; Matheus,Dácio Roberto.
Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Peroxidases; Laccase; Solid-state fermentation; "shimeji" culture residue.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400013
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APPLICATION OF SOLID-STATE FERMENTATION FOR CELLULASE ENZYME PRODUCTION USING TRICHODERMA VIRIDE AgEcon
Mojsov, Kiro.
The Solid-state fermentation (SSF) is alternative to submerged fermentation for production antibiotics, single cell protein, enzymes, organic acids, biofuel, etc. However, the advantages of SSF in various processes are found to be greater than in submerged fermentation. This technique not only decreases the cost of the process but also makes product cheaper for consumers. The paper describes experimental application of SSF on wheat straw for production of mycelia protein and cellulase enzymes by Trichoderma viride. This actual waste from agriculture industry was used as a nourishing base by Trichoderma viride in SSF for cellulase enzyme production. Growth and enzymes production by Trichoderma viride were evaluated on wheat straw and alkali treatment...
Tipo: Journal Article Palavras-chave: Solid-state fermentation; Trichoderma viride; Cellulase enzyme; Wheat straw agro-waste; Single cell protein.; Research and Development/Tech Change/Emerging Technologies; L65.
Ano: 2010 URL: http://purl.umn.edu/92392
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The use of alpha-methyl-D-glucoside, a synthetic analogue of maltose, as inducer of amylase by Aspergillus sp in solid-state and submerged fermentations BJM
Moreira,Fabiana G.; Lenartovicz,Veridiana; Souza,Cristina G.M. de; Ramos,Edivan P.; Peralta,Rosane M..
The use of a methyl-D-glucoside (alphaMG), a synthetic analogue of maltose, as carbon source and inducer of amylase synthesis to several species of Aspergillus was studied in submerged and solid-state fermentations. Among a group of ten species, A. tamarii, A. fumigatus and A. flavus were able to produce biomass and high specific amylolytic activity in submerged cultures containing alphaMG as the only carbon source. In solid state fermentation, the enrichment of basal wheat bran or corn cob medium with alphaMG increased up to 3 times the production of amylases. In both submerged and solid state fermentations, alphaMG was more effective inducer of amylases than maltose and starch.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Aspergillus; Solid-state fermentation; Submerged fermentation.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100004
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Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation BJM
Moretti,Marcia M. S.; Bocchini-Martins,Daniela A.; Silva,Roberto Da; Rodrigues,André; Sette,Lara D.; Gomes,Eleni.
Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermophilic fungi; Solid-state fermentation; Xylanase; Cellulase.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300032
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Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate BJM
Mrudula,Soma; Murugammal,Rangasamy.
Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase & FPase activities were found to be 3.29 and 2.3 U per ml culture broth, respectively. The productivity of extracellular cellulase in SSF was 14.6 fold higher than in SmF. The physical and nutritional parameters of fermentation like pH, temperature, substrate, carbon and nitrogen sources were optimized. The optimal conditions for maximum biosynthesis of cellulase by A. niger were shown to be at pH 6,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Coir waste; Cellulase; Submerged fermentation; Solid-state fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300033
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Co-Production of Nattokinase and Poly (γ-Glutamic Acid) Under Solid-State Fermentation Using Soybean and Rice Husk BABT
Nie,Guangjun; Zhu,Zhubin; Liu,Fang; Nie,Zhijie; Ye,Yuchao; Yue,Wenjin.
ABSTRACTThe aim of this work was to study the co-production of nattokinase and poly (γ-glutamic acid) by Bacillus subtilis natto with soybean and rice husk under solid-state fermentation (SSF). The results showed that the size of soybean particle and rice husk significantly improved the co-production of nattokinase and poly (γ-glutamic acid), yielding 2503.4 IU/gs and 320 mg/gs, respectively in the improved culture medium composed of 16.7% soybean flour and 13.3% rice husk with 70% water content. The yields increased by approximate 7- and 2-fold factor relative to their original ones. Thus, the co-production of nattokinase and poly (γ-glutamic acid) under SSF could be considered as an efficient method to exploit agro-residues for economical production of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-production; Nattokinase; Poly (γ-glutamic acid); Rice husk; Solid-state fermentation.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500718
Registros recuperados: 40
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