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Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)-based scaffolds for tissue engineering BJMBR
Chang,H.M.; Wang,Z.H.; Luo,H.N.; Xu,M.; Ren,X.Y.; Zheng,G.X.; Wu,B.J.; Zhang,X.H.; Lu,X.Y.; Chen,F.; Jing,X.H.; Wang,L..
Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: PHBHHx; Tissue engineering; Biomaterial.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700533
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Viability Assays of PLLA Fibrous Membranes Produced by Rotary Jet Spinning for Application in Tissue Engineering BABT
Ciocca,Bárbara Etruri; Munhoz,André Luiz Jardini; Cardoso,Guinea Brasil Camargo; Rodigues,Ana Amélia; Pattaro,Ana Flávia; Kaasi,Andreas; Maciel Filho,Rubens.
Abstract Tissue engineering suggests different forms to reconstruct tissues and organs. One of the ways is through the use of polymeric biomaterials such as poly(L-lactic acid) (PLLA). PLLA is a recognized material in tissue engineering due to its characteristics as biocompatibility and bioresorbability. In this work PLLA fibrous membranes were produced by a simple technique known as rotary jet spinning. The rotary jet spinning consists of fibrous membranes production, with fibers of scale nano/micrometric, from a polymeric solution through the centrifugal force generated by the equipment. The membranes formed were subjected to preliminary in vitro assays to verify the cytotoxicity of the membranes made in contact with the cells. Direct cytotoxicity assays...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue engineering; Poly(L-lactic acid); Biomaterial; Rotary jet spinning; In vitro assay.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100604
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Control of the skin scarring response Anais da ABC (AABC)
Ferreira,Lydia M.; Gragnani,Alfredo; Furtado,Fabianne; Hochman,Bernardo.
There comes a time when the understanding of the cutaneous healing process becomes essential due to the need for a precocious tissue repair to reduce the physical, social, and psychological morbidity. Advances in the knowledge on the control of interaction among cells, matrix and growth factors will provide more information on the Regenerative Medicine, an emerging area of research in medical bioengineering. However, considering the dynamism and complexity of the cutaneous healing response, it is fundamental to understand the control mechanism exerted by the interaction and synergism of both systems, cutaneous nervous and central nervous, via hypothalamus hypophysis-adrenal axis, a relevant subject, but hardly ever explored. The present study reviews the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Keratinocytes; Fibroblasts; Keloid; Tissue engineering; Skin; Plastic surgery.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300024
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Silanisation de polysaccharides en milieu liquide ionique et réactivité d’un organoalkoxysilane vis-à-vis de nucléophiles simples : vers des hydrogels injectables pour l’ingénierie tissulaire du cartilage ArchiMer
Guillory, Xavier.
Tissue engineering is a real challenge for the treatment of cartilage pathologies. In this field, biomimetic hydrogels based on natural polymers are among the most commonly used matrices. A hydrogel made of silanized hydroxypropylmethylcellulose (HPMC-Si) is especially promising because it can be injected in cartilaginous lesions by minimally invasive surgery. However, the current synthesis of HPMC-Si is limited by the insolubility of hydroxypropylmethylcellulose (HPMC). This thesis work was focused on finding new synthesis conditions for the design of HPMC-Si hydrogel. In order to obtain a complete solubilization of HPMC and to improve its functionalization by the (3-glycidyloxypropyl) trimethoxysilane (GPTMS), the use of ionic liquids (IL), which are...
Tipo: Text Palavras-chave: Silanisation; Biopolymères; Polysaccharides; Liquides ioniques; Chimie sol-gel; Ingénierie tissulaire; Hydrogels.; Silanization; Biopolymers; Polysaccharides; Ionic liquids; Sol-gel chemistry; Tissue engineering; Hydrogels..
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00341/45188/44585.pdf
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Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing Biocell
Imani,Rana; Hojjati Emami,Shahriar; Fakhrzadeh,Hossein; Baheiraei,Nafiseh; Sharifi,Ali M.
The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. The size and viability of the aggregates obtained at different initial cell densities and pre-culture duration were compared. The proliferative ability of the cell aggregates and their ability to spread in culture plates were also investigated. In both methods, the optimum average size of the aggregates was less than 500 µm. CT aggregates were smaller than HD aggregates....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue engineering; Hanging drop; Conical tube; Bioprinting.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000100003
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Blood compatibility assessments of electrospun polyurethane nanocomposites blended with megni oil for tissue engineering applications Anais da ABC (AABC)
JAGANATHAN,SARAVANA KUMAR; MANI,MOHAN P.; SUPRIYANTO,EKO.
Abstract: Tissue engineering holds as a prominent technique to repair or replace the damaged human parts to recreate its native function. In this research, a novel scaffold based on polyurethane (PU) comprising megni oil was electrospun for tissue engineering applications. The obtained polyurethane blended with megni oil nanofibers were characterized by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), contact angle measurement and atomic force microscopy (AFM). Furthermore, the blood compatibility of the fabricated nanocomposites evaluated through activated prothrombin time (APTT), partial thromboplastin time (PT) and hemolysis assay to determine the anticoagulant nature. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyurethane; Megni oil; Electrospun scaffold; Blood compatibility; Tissue engineering.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300708
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An improved in vivo method for atrioventricular node ablation via thoracotomy BJMBR
MacIver,R.H.; Stewart,R.D.; Backer,C.L.; Tsao,S.; Harrington,D.A.; Mavroudis,C..
The atrioventricular (AV) node is permanently damaged in approximately 3% of congenital heart surgery operations, requiring implantation of a permanent pacemaker. Improvements in pacemaker design and in alternative treatment modalities require an effective in vivo model of complete heart block (CHB) before testing can be performed in humans. Such a model should enable accurate, reliable, and detectable induction of the surgical pathology. Through our laboratory’s efforts in developing a tissue engineering therapy for CHB, we describe here an improved in vivo model for inducing chronic AV block. The method employs a right thoracotomy in the adult rabbit, from which the right atrial appendage may be retracted to expose an access channel for the AV node. A...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Atrioventricular node; Heart block; Ablation; Tissue engineering; Regenerative medicine; In vivo model.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000200012
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Biocompatibility of Human Auricular Chondrocytes Cultured onto a Chitosan/Polyvynil Alcohol/Epichlorohydrin-Based Hydrogel for Tissue Engineering Application International Journal of Morphology
Melgarejo-Ramírez,Yaaziel; Sánchez-Sánchez,Roberto; García-Carvajal,Zaira; García-López,Julieta; Gutiérrez-Gómez,Claudia; Luna-Barcenas,Gabriel; Ibarra,Clemente; Velasquillo,Cristina.
Tissue engineering (TE) has become an alternative for auricular reconstruction based on the combination of cells, molecular signals and biomaterials. Scaffolds are biomaterials that provide structural support for cell attachment and subsequent tissue development. Ideally, a scaffold should have characteristics such as biocompatibility and bioactivity to adequate support cell functions. Our purpose was to evaluate biocompatibility of microtic auricular chondrocytes seeded onto a chitosan-polyvinyl alcohol-epichlorohydrin (CS-PVA-ECH) hydrogel to propose this material as a scaffold for tissue engineering application. After being cultured onto CS-PVA-ECH hydrogels, auricular chondrocytes viability was up to 81%. SEM analysis showed cell attachment and...
Tipo: Journal article Palavras-chave: Elastic cartilage; Auricular reconstruction; Chitosan hydrogel; Tissue engineering; Biocompatibility.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400036
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Decellularized Bovine Articular Cartilage Matrix Reinforced by Carboxylated-SWCNT for Tissue Engineering Application BABT
Mohammadie,Zari Majidi; Parivar,Kazem; Shahri,Nasser Mahdavi; Fereidoni,Masoud; Hayati-Roodbari,Nasim.
ABSTRACT Nanotubes with their unique properties have diversified mechanical and biological applications. Due to similarity of dimensions with extracellular matrix (ECM) elements, these materials are used in designing scaffolds. In this research, Carboxylated Single-Wall Carbon Nanotubes in optimization of decellularized scaffold of bovine articular cartilage was used. At first, the articular cartilage was decellularized. Then the scaffolds were analyzed in: (i) decellularized scaffolds, and (ii) scaffolds plunged into homogenous suspension of nanotubes in distilled water, were smeared with Carboxylated-SWCNT. The tissue rings derived from the rabbit's ear were assembled with reinforced scaffolds and they were placed in a culture media for 15 days. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Articular cartilage; Carboxylated-SWCNT; Extracellular matrix; Natural scaffold; Tissue engineering.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100607
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Expression of Mesenchymal Stem Cell Phenotype in Human Nasal Respiratory Epithelial Cells International Journal of Morphology
Muhammed,Aisha A; Yunus,Mohd Heikal M; Saim,Aminuddin B; Idrus,Ruszymah B. H.
The respiratory epithelium is the first line of contact with the external hazards. Thus it can be damaged and need to be replaced to avoid healing by fibrosis. Tracheal tissue engineering is an alternative promising treatment modality. Mesenchymal stem cell markers are surface proteins, which are responsible for some of these cells unique properties. The objective of this study was to detect the mesenchymal stem cell phenotype among the human nasal respiratory epithelial cells via two immunophenotyping techniques. Respiratory epithelial cells were cultured using co-culture technique, fibroblasts was removed at confluence leaving respiratory epithelial cells, which were passage further to passage 4. Cells were evaluated for mesenchymal stem cell markers...
Tipo: Journal article Palavras-chave: Human nasal respiratory epithelium; Immunostaining; Mesenchymal stem cells marker; Tissue engineering; Nasal turbinate.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400047
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Co-culture of chondrocytes and bone marrow mesenchymal stem cells in vitro enhances the expression of cartilaginous extracellular matrix components BJMBR
Qing,Chang; Wei-ding,Cui; Wei-min,Fan.
Chondrocytes and bone marrow mesenchymal stem cells (BMSCs) are frequently used as seed cells in cartilage tissue engineering. In the present study, we determined if the co-culture of rabbit articular chondrocytes and BMSCs in vitro promotes the expression of cartilaginous extracellular matrix and, if so, what is the optimal ratio of the two cell types. Cultures of rabbit articular chondrocytes and BMSCs were expanded in vitro and then cultured individually or at a chondrocyte:BMSC ratio of 4:1, 2:1, 1:1, 1:2, 1:4 for 21 days and cultured in DMEM/F12. BMSCs were cultured in chondrogenic induction medium. Quantitative real-time RT-PCR and Western blot were used to evaluate gene expression. In the co-cultures, type II collagen and aggrecan expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-culture; Bone mesenchymal stem cells; Chondrocytes; Tissue engineering.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000400006
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Enriching a cellulose hydrogel with a biologically active marine exopolysaccharide for cell-based cartilage engineering ArchiMer
Rederstorff, E.; Rethore, G.; Weiss, P.; Sourice, S.; Beck-cormier, S.; Mathieu, E.; Maillasson, M.; Jacques, Y.; Colliec-jouault, Sylvia; Fellah, B. H.; Guicheux, J.; Vinatier, C..
The development of biologically and mechanically competent hydrogels is a prerequisite in cartilage engineering. We recently demonstrated that a marine exopolysaccharide, GY785, stimulates the in vitro chondrogenesis of adipose stromal cells. In the present study, we thus hypothesized that enriching our silated hydroxypropyl methylcellulose hydrogel (Si-HPMC) with GY785 might offer new prospects in the development of scaffolds for cartilage regeneration. The interaction properties of GY785 with growth factors was tested by surface plasmon resonance (SPR). The biocompatibility of Si-HPMC/GY785 towards rabbit articular chondrocytes (RACs) and its ability to maintain and recover a chondrocytic phenotype were then evaluated in vitro by MTS assay, cell counting...
Tipo: Text Palavras-chave: Cartilage; Hydrogel; Glycosaminoglycan; Marine polysaccharides; Chondrocytes; Tissue engineering.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00303/41449/40650.pdf
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Building bone tissue: matrices and scaffolds in physiology and biotechnology BJMBR
Riminucci,M.; Bianco,P..
Deposition of bone in physiology involves timed secretion, deposition and removal of a complex array of extracellular matrix proteins which appear in a defined temporal and spatial sequence. Mineralization itself plays a role in dictating and spatially orienting the deposition of matrix. Many aspects of the physiological process are recapitulated in systems of autologous or xenogeneic transplantation of osteogenic precursor cells developed for tissue engineering or modeling. For example, deposition of bone sialoprotein, a member of the small integrin-binding ligand, N-linked glycoprotein family, represents the first step of bone formation in ectopic transplantation systems in vivo. The use of mineralized scaffolds for guiding bone tissue engineering has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular matrix; Bone matrix; Bone formation; Biomaterials; Tissue engineering.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800008
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Single-walled carbon nanotubes functionalized with sodium hyaluronate enhance bone mineralization BJMBR
Sá,M.A.; Ribeiro,H.J.; Valverde,T.M.; Sousa,B.R.; Martins-Júnior,P.A.; Mendes,R.M.; Ladeira,L.O.; Resende,R.R.; Kitten,G.T.; Ferreira,A.J..
The aim of this study was to evaluate the effects of sodium hyaluronate (HY), single-walled carbon nanotubes (SWCNTs) and HY-functionalized SWCNTs (HY-SWCNTs) on the behavior of primary osteoblasts, as well as to investigate the deposition of inorganic crystals on titanium surfaces coated with these biocomposites. Primary osteoblasts were obtained from the calvarial bones of male newborn Wistar rats (5 rats for each cell extraction). We assessed cell viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay and by double-staining with propidium iodide and Hoechst. We also assessed the formation of mineralized bone nodules by von Kossa staining, the mRNA expression of bone repair proteins, and the deposition of inorganic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoblast; Nanotechnology; Carbon nanotubes; Sodium hyaluronate; Tissue engineering; Osseointegration.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200602
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Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues ArchiMer
Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia.
Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800...
Tipo: Text Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf
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Marine Polysaccharides: A Source of Bioactive Molecules for Cell Therapy and Tissue Engineering ArchiMer
Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia.
The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the...
Tipo: Text Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf
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Using mesenchymal stem cells as a therapy for bone regeneration and repairing Biol. Res.
Shao,Jin; Zhang,Weiwei; Yang,Tieyi.
Bone is a unique tissue which could regenerate completely after injury rather than heal itself with a scar. Compared with other tissues the difference is that, during bone repairing and regeneration, after the inflammatory phase the mesenchymal stem cells (MSCs) are recruited to the injury site and differentiate into either chondroblasts or osteoblasts precursors, leading to bone repairing and regeneration. Besides these two precursors, the MSCs can also differentiate into adipocyte precursors, skeletal muscle precursors and some other mesodermal cells. With this multiline-age potentiality, the MSCs are probably used to cure bone injury and other woundings in the near future. Here we will introduce the recent developments in understanding the mechanism of...
Tipo: Journal article Palavras-chave: Mesenchymal stem cells; Bone regeneration; Bone repairing; Tissue engineering.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100062
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Delivery of the Sox9 gene promotes chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in an in vitro model BJMBR
Wang,Z.H.; Li,X.L.; He,X.J.; Wu,B.J.; Xu,M.; Chang,H.M.; Zhang,X.H.; Xing,Z.; Jing,X.H.; Kong,D.M.; Kou,X.H.; Yang,Y.Y..
SRY-related high-mobility-group box 9 (Sox9) gene is a cartilage-specific transcription factor that plays essential roles in chondrocyte differentiation and cartilage formation. The aim of this study was to investigate the feasibility of genetic delivery of Sox9 to enhance chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells (hUC-MSCs). After they were isolated from human umbilical cord blood within 24 h after delivery of neonates, hUC-MSCs were untreated or transfected with a human Sox9-expressing plasmid or an empty vector. The cells were assessed for morphology and chondrogenic differentiation. The isolated cells with a fibroblast-like morphology in monolayer culture were positive for the MSC markers CD44, CD105,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic modification; Tissue engineering; Stem cells; Sox9; Chondrogenesis.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400279
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In vitro cultivation of canine multipotent mesenchymal stromal cells on collagen membranes treated with hyaluronic acid for cell therapy and tissue regeneration BJMBR
Wodewotzky,T.I.; Lima-Neto,J.F.; Pereira-Júnior,O.C.M.; Sudano,M.J.; Lima,S.A.F.; Bersano,P.R.O.; Yoshioka,S.A.; Landim-Alvarenga,F.C..
Support structures for dermal regeneration are composed of biodegradable and bioresorbable polymers, animal skin or tendons, or are bacteria products. The use of such materials is controversial due to their low efficiency. An important area within tissue engineering is the application of multipotent mesenchymal stromal cells (MSCs) to reparative surgery. The combined use of biodegradable membranes with stem cell therapy may lead to promising results for patients undergoing unsuccessful conventional treatments. Thus, the aim of this study was to test the efficacy of using membranes composed of anionic collagen with or without the addition of hyaluronic acid (HA) as a substrate for adhesion and in vitro differentiation of bone marrow-derived canine MSCs. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Collagen scaffolds; Biomaterials; Hyaluronic acid; Mesenchymal stromal cells; Tissue engineering; Cell culture.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001200008
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Viability of mesenchymal stem cells during electrospinning BJMBR
Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P..
Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006
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