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A new variety of Bacopa monnieri obtained by in vitro polyploidization Electron. J. Biotechnol.
Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol.
The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences...
Tipo: Journal article Palavras-chave: Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002
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A NEW WORLD MONKEY MICROSATELLITE (AP74) HIGHLY CONSERVED IN PRIMATES Acta biol.Colomb.
OKLANDER,LUCIANA INÉS; STEINBERG3,ELIANA RUTH; DOLORES MUDRY,MARTA.
Given their great variability, microsatellites or STRs became the most commonly used genetic markers over the last 15 years. The analysis of these markers requires minimum quantities of DNA, allowing the use of non invasive samples, such as feces or hair. We amplified the microsatellite Ap74 in blood and hair samples in order to analyze the levels of genomic conservation among a wide range of primates including: Lemur catta, Alouatta caraya, Ateles belzebuth, Ateles chamek, Pan troglodytes, Papio sp., and Homo sapiens. In all cases we obtained amplification products that exhibited similar size both in monkeys and human (oscillating between 126 and 176 bp), except in the lemur where the detected fragment presented a size of approximately 1000 bp. The...
Tipo: Journal article Palavras-chave: Primates; Microsatellite markers; PCR amplification; Non-invasive sampling; Genomic conservation; Genetic variability.
Ano: 2012 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2012000100007
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A note on stability in food matrices of Salmonella enterica serovar Enteritidis-controlling bacteriophages Electron. J. Biotechnol.
Robeson,James; Turra,Gabriel; Huber,Karen; Borie,Consuelo.
Background Lytic bacteriophages are bacterial viruses that upon infection kill their host cells and therefore have re-emerged as biological control agents of bacterial pathogens, particularly in the field of food related infections. Here, we investigated the stability in different food matrices of five phage isolates capable of controlling the foodborne pathogen Salmonella enterica serovar Enteritidis (SE). Results We found that two phages, originally isolated from food sources, were up to 5 logs more stable than three phages isolated from sewage, in ten food matrices (fresh and processed) at both 4°C and 18°C. Conclusion Lytic phages isolated from contaminated food sources seem to be a better choice when structuring phage cocktails to be used in the...
Tipo: Journal article Palavras-chave: Biocontrol; Foodstuffs; Phage.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400008
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A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization Electron. J. Biotechnol.
Wei,Lei; Yin,Li; Hu,Xiaole; Xu,Ying; Chen,Fang.
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pathway involved in the biosynthesis of terpenoids. The present investigation describes the cloning, characterization and subcellular localization of isopentenyl diphosphate isomerase (IPI) gene from J. curcas. IPI is one of the rate limiting enzymes in the biosynthesis of terpenoids, catalyzing the crucial interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Results A full-length JcIPI cDNA consisting of 1355 bp was cloned....
Tipo: Journal article Palavras-chave: Heterologous expression; Southern blot; Terpenoids; Transient expression.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600007
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A Novel Method for Localizing Nerve Entry Points During Spasticity Treatment International Journal of Morphology
Yang,Shengbo; Hu,Shuangjiang; Tian,Xufeng; Hu,Shuaiyu.
The nerve entry points (NEPs) cannot be accurately localized for the treatment of muscle spasticity in neurolysis. The aim of this study was to develop a new method of accurately localizing nerve entry points in relation to bony landmarks. NEPs in human cadavers were coated with barium sulfate. Method 1 consisted of horizontal and longitudinal reference lines being designated based on bony landmarks, followed by radiography and measurements using picture archiving and communication system software. Method 2 involved sewing the barium sulfate-soaked suture thread into the skin to designate the horizontal and longitudinal reference lines, followed by computed tomography (CT) scanning to determine the skin surface location and puncture depth for the NEPs....
Tipo: Journal article Palavras-chave: Spasticity; Nerve entry points; Anatomy; X-ray; Localization.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300002
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A Novel Path of Communication from Kidney to the Adrenal Gland in Bactrian Camels (Camelus bactrianus) International Journal of Morphology
Ye,Wenling; Wang,Fengling; Lv,Shuangyu; Wang,Yange; Dong,Shuo; Wang,Jianlin.
The adrenal gland is an important endocrine organ in vertebratesthat produces a wide variety of hormones. The anatomical results showed that the adrenal gland in Bactrian camel had a typical blood supply and innervation. Surprisingly, we found that there was a novel connection mainly consisted of fibrous tissue, blood vessels and nerve bundles between the adrenal gland and the kidney in Bactrian camel, and we named it fibrous tissue-blood vessels-nerve bundles (FBN bundle). To the best of our knowledge, this FBN bundle was the first reported in Bactrian camel, and not yet observed in other animals. While, its functions and mechanisms should be studied further. We speculated that a direct function of this novel path might be to strengthen the communication...
Tipo: Journal article Palavras-chave: Anatomical; Adrenal gland; FBN bundle; Blood supply; Bactrian camels.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400044
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A novel pH-stable, endoglucanase (JqCel5A) isolated from a salt-lake microorganism, Jonesia quinghaiensis Electron. J. Biotechnol.
Lin,Ling; Liu,Xiaozhou; Zhou,Yating; Guan,Linyan; Jiajia,He; Huang,Weiqian.
Background: Endoglucanase, one of three type cellulases, can randomly cleave internal p-1,4-linkages in cellulose polymers. Thus, it could be applied in agricultural and industrial processes. Results: A novel endoglucanase gene (JqCel5A) was cloned from Jonesia quinghaiensis and functionally expressed in Escherichia coli Rosetta (DE3). It contained 1722 bp and encoded a 573-residue polypeptide consisting of a catalytic domain of glycoside hydrolase family 5 (GH5) and a type 2 carbohydrate-binding module (CBM2), together with a predicted molecular mass of 61.79 kD. The purified JqCel5A displayed maximum activity at 55°C and pH 7.0, with 21.7 U/mg, 26.19 U/mg and 4.81 U/mg towards the substrate carboxymethyl cellulose, barley glucan and filter paper,...
Tipo: Journal article Palavras-chave: Protein modeling Site-directed mutagenesis Cellulases Recombinant endoglucanase gene Catalytic domain ofglycoside hydrolase Carbohydrate-binding module High pH stability Tolerance to deleterious chemicals; Tolerance to heavy metals Tolerance to detergents.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600009
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A PBL MODEL WITH ORGANIZED LARGE EDDIES FOR MESOSCALE-SYNOPTIC-GLOBAL WINDS Gayana
Brown,Robert A.
The University of Washington PBL model is a nonlinear similarity model (with organized large eddies in an equilibrium mean flow) suitable for km-scale to General Circulation Model or Climate numerical models. The theory and verification for this solution are now well established. Researchers who work with winds in the boundary layer with scales from point to 20-km regimes must be aware of this horizontal inhomogenuity in the PBL flow to avoid significant errors. Since the OLE contribute advectively to the mean flow there can be 10-20% (or more) increase in surface winds and fluxes over K-theory models. The model is described and several applications have been made
Tipo: Journal article
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382004000200010
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A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors Electron. J. Biotechnol.
Yulita,Kusumadewi Sri; Kouno,Takafumi; Ezaki,Bunichi.
Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. These phage particles are hybridized with a biotinylated target DNA fragment which is immobilized on the surface of streptavidin paramagnetic particle (SA-PMP). The phage particles are released from the target DNA fragment by a nuclease treatment and the recovered phages are used to the next round of hybridization. These processes are repeated three times to amplify the target phages in the population. This simple method is faster, and more systemic than other...
Tipo: Journal article Palavras-chave: AtGST11 gene; Biopanning; DNA binding proteins; T7 phage differential display; Transcription factors.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014
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A Pilot Study of the Mandibular Angle and Ramus in Indian Population International Journal of Morphology
Rai,Rajalakshmi; Ranade,Anu Vinod; Prabhu,Latha Venkatraya; Pai,Mángala M; Madhyastha,Sampath; Kumaran,Mángala.
To evaluate the mandibular angle and to analyze the relationship of the angle and height & breadth of the ramus of the mandible to the gender, so as to study its role in the anthropological diagnosis. The angle, height and breadth of the ramus of adult dry human mandibles of both sexes were measured using a goniometer. The values obtained were analyzed statistically. The present study showed a statistically significant difference in the mandibular angle as well as height of the ramus between both the sexes. The mean mandibular angle of Indian population when compared to that of European population was found to be lower by 9 degrees. The findings of this study might be useful in providing anthropological data that can also be used in dental and...
Tipo: Journal article Palavras-chave: Mandíbula; Mandibular angle; Ramus of the Mandible; Indian population.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200019
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A pilot study on genetic variation in purine-rich elements in the nephrin gene promoter in type 2 diabetic patients Biol. Res.
GONZÁLEZ,RODRIGO; TIRADO,ARES; BALANDA,MONSERRAT; ALVO,MIRIAM; BARQUÍN,INÉS; DURRUTY,PILAR; LOBOS,SERGIO; SEELENFREUND,DANIELA.
Diabetic nephropathy (DN) is one of the major complications of type 2 diabetes and is associated with coronary disease. Nephrin, a protein mainly expressed in glomeruli, is decreased in DN and other kidney diseases. Since insulin levels are misregulated in type 2 diabetes, a possible connection between DN and its decreased nephrin expression could be the presence of regulatory elements responsive to insulin in the nephrin gene (NPHS1) promoter region. In this work, using bioinformatic tools, we identified a purine-rich GAGA element in the nephrin gene promoter and conducted a genomic study in search of the presence of polymorphisms in this element and its possible association with DN in type 2 diabetic patients. We amplified and sequenced a 514 bp promoter...
Tipo: Journal article Palavras-chave: GAGA box; Gene promoter; Nephrin; Polymorphism.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400010
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A POLYCLONAL -ANTIBODY-IMMUNOPEROXIDASE-CONJUGATE FOR THE SPECIFIC DETECTION OF PORCINE CIRCOVIRUS TYPE 2 Rev Salud Anim.
Pérez,L.J.; Díaz de Arce,Heidy; Barrera,Maritza; Castell,Sara; Frías,María T.
Porcine circovirus 2 is nowadays accepted as the essential infectious agent of postweaning multisystemic wasting syndrome which causes severe economic losses in porcine production worldwide. The diagnosis of PMWS is a difficult task and must follow three criteria: (i) the presence of compatible clinical signs, (ii) the presence of characteristic microscopic histopathological lesions, and (iii) the presence of PCV2 within these lesions in moderate or high. The presence of PCV2 in lymphoid tissues must be demonstrated by in situ hybridization or immunohistochemical methods. The in situ hybridization is a more complex and expense compared to other diagnostic tools, on the other hand, one problem concerning the immunohistochemical methods for PMWS diagnostic...
Tipo: Journal article Palavras-chave: Porcine circovirus 2; Immunohistochemical; Conjugate; Polyclonal antibody.
Ano: 2009 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2009000300003
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A possible role of Drosophila CTCF in mitotic bookmarking and maintaining chromatin domains during the cell cycle Biol. Res.
Shen,Wenlong; Wang,Dong; Ye,Bingyu; Shi,Minglei; Zhang,Yan; Zhao,Zhihu.
BACKGROUND: The CCCTC-binding factor (CTCF) is a highly conserved insulator protein that plays various roles in many cellular processes. CTCF is one of the main architecture proteins in higher eukaryotes, and in combination with other architecture proteins and regulators, also shapes the three-dimensional organization of a genome. Experiments show CTCF partially remains associated with chromatin during mitosis. However, the role of CTCF in the maintenance and propagation of genome architectures throughout the cell cycle remains elusive. RESULTS: We performed a comprehensive bioinformatics analysis on public datasets of Drosophila CTCF (dCTCF). We characterized dCTCF-binding sites according to their occupancy status during the cell cycle, and identified...
Tipo: Journal article Palavras-chave: DCTCF; Cell cycle; Chromatin domains; Mitotic bookmarking; Bioinformatics.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100027
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A potential nutritional modifier for predicting primary productivity of Pinus radiata in New Zealand using a simplified radiation-use efficiency model Ciencia e Investigación Agraria
Bown,Horacio E; Mason,Euan G; Watt,Michael S; Clinton,Peter W.
H.E. Bown, E.G. Mason, M.S. Watt, and P.W. Clinton. 2013. A potential nutritional modifier for predicting primary productivity of Pinus radiata in New Zealand using a simplified radiation-use efficiency model. Cien. Inv. Agr. 40(2): 361-374. The 3-PG (Physiological Principles in Predicting Growth) radiation-use efficiency model has been widely used and tested for predicting the primary productivity of forests all over the world. This radiation-use efficiency model accounts for plant nutrition through a user-defined dimensionless fertility parameter (fN) that determines the effects a unit of radiation. Currently, this fertility parameter has to be entered by the user based on intuition or experience. The goal of this study was to propose a fertility...
Tipo: Journal article Palavras-chave: Nutritional modifier; Pinus radiata; Primary productivity; Radiation-use efficiency; Soil C/N; Soil N.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000200011
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A practical approach to the understanding and teaching of RNA silencing in plants Electron. J. Biotechnol.
Bazzini,Ariel A; Mongelli,Vanesa C; Hopp,H. Esteban; del Vas,Mariana; Asurmendi,Sebastián.
Gene silencing, also called RNA interference (RNAi) is a specific mechanism of RNA degradation involved in gene regulation, development and defense in eukaryotic organisms. It became an important subject in the teaching programs of molecular biology, genetics and biotechnology courses in the last years. The aim of this work is to provide simple and inexpensive assays to understand and teach gene silencing using plants as model systems. The use of transient and permanent transgenic plants for expressing reporter genes, like those derived from jellyfish green fluorescent protein (gfp) encoding gene, provides a nice, colorful and conclusive image of gene silencing. Three experimental approaches to evidence RNA silencing are depicted. In the first approach...
Tipo: Journal article Palavras-chave: Gene silencing; GFP; HC-Pro; PDS; Protocols; PTGS; Teaching; Transgenic; Transient expression; TRV; VIGS.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200002
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A predicted structure of the cytochrome c oxidase from Burkholderia pseudomallei Electron. J. Biotechnol.
Mohd. Raih,Mohd. Firdaus; Sailan,Ahmad Tarmidi; Zamrod,Zulkeflie; Embi,Mohd. Noor; Mohamed,Rahmah.
Cytochrome c oxidase, the terminal enzyme of the respiratory chains of mitochondria and aerobic bacteria, catalyzes electron transfer from cytochrome c to molecular oxygen. The enzyme belongs to the haem-copper-containing oxidases superfamily. A recombinant plasmid carrying a 2.0 kb insert from a Burkholderia pseudomallei genomic library was subjected to automated DNA sequencing utilizing a primer walking strategy. Analysis of the 2002 bp insert revealed a 1536 bp open reading frame predicted to encode a putative cytochrome c oxidase. Further analysis using sequence alignments and tertiary structure analysis tools demonstrated that the hypothetical B. pseudomallei cytochrome c oxidase is similar to cytochrome c oxidases from other organisms such as Thermus...
Tipo: Journal article
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000100005
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A preferable approach to clone hLIF cDNA from the genomic DNA Electron. J. Biotechnol.
Cui,Yihong; Zhu,Guangqin; Chen,Qiuju; Wang,Yunfei; Yang,Mingming; Song,Yuxuan; Wang,Jiangang; Cao,Binyun.
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the research of life science, but it is difficult to obtain by traditional reverse transcription. In this study, we employed a novel strategy to clone the human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA directly isolated from the mucous membrane of mouth. The hLIF sequence can be acquired within a few hours by means of amplification of each exon and splicing using overlap-PCR. Thus, the new approach developed in this study is simple, time- and cost-effective, and it is not limited to particular gene expression levels of each tissue.
Tipo: Journal article Palavras-chave: CDNA cloning; HLIF; Oral mucous membrane; Overlap PCR.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300012
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A Pyranine based Procedure for Evaluation of the Total Antioxidant Potential (TRAP) of Polyphenols: A Comparison with closely related Methodologies Biol. Res.
CAMPOS,ANA MARÍA; SOTOMAYOR,CARLOS PATRICIO; PINO,EDUARDO; LISSI,EDUARDO.
A novel procedure for the evaluation of total reactive antioxidant potentials (TRAP) is described. The method is based on the measurement of the bleaching of pyranine by peroxyl radicals. The addition of the antioxidants produces a clear induction time whose magnitude is directly related to the antioxidant concentration. A comparison of the values obtained with those reported employing closely related methodologies shows that the results are significantly affected by the substrate employed to monitor the steady state free radical concentration. Possible sources of this dependence are discussed
Tipo: Journal article Palavras-chave: Peroxyl radicals; Polyphenols; Pyranine; Total reactive antioxidant potential.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200015
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A quantitative analysis of forest fragmentation in Los Tuxtlas, southeast Mexico: patterns and implications for conservation RChHN
MENDOZA,EDUARDO; FAY,JOHN; DIRZO,RODOLFO.
Habitat loss is a critical threat to tropical biodiversity and its quantification constitutes a central conservation issue. Typically, assessments have been based on deforestation rates statistics. However, this overlooks the effects brought about by the spatial reconfiguration of the remaining habitat: fragmentation. We present an analysis of fragmentation in a Neotropical site aimed at: (a) devising a protocol for its quantification, (b) using such protocol to provide insights on the ecological consequences of fragmentation, (c) exploring its applicability to address the hypothesis that forest size-inequality decreases with elevation, an indicator of habitat accessibility. We applied the Gini coefficient (G) and the Lorenz curve to analyze fragment-size...
Tipo: Journal article Palavras-chave: Edge effect; Fragment shape; Gini coefficient; Lorenz curve; Tropical rain forest.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2005000300008
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A Quantitative Assessment of the Morphological Characteristics of BeWo Cells as an in vitro Model of Human Trophoblast Cells International Journal of Morphology
Abaidoo,C.S; Warren,M. A; Andrews,P. W; Boateng,K. A.
In order to study the detailed morphology of trophoblast cells during human implantation, BeWo cells were cultured as spheroids in suspension culture. These cultures were then processed for light and electron microscopical examination. The present study showed that the BeWo spheroids consist of two cell types which are cytotrophoblast-like and syncytiotrophoblast-like. The cells with larger nuclear diameter made up only about 1% of the cell population and appear to be those of syncytiotrophoblast. Therefore the predominant cell type of the BeWo spheroids appeared to be relatively undifferentiated and cytotrophoblast-like. About 10% of the BeWo cells in the present study were mitotic, indicating a highly proliferative population. Total cell number increased...
Tipo: Journal article Palavras-chave: BeWo; Trophoblast; Morphometry; Spheroids.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400011
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