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Registros recuperados: 28
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A Field study to Estimate the Prevalence of Bovine African Trypanosomosis in Butaleja District, Uganda OAK
JING, Zhang; MAGONA, Joseph W; SAKURAI, Tatsuya; THEKISOE, Oriel M. M; OTIM, Charles P; SUGIMOTO, Chihiro; INOUE, Noboru; 井上, 昇.
Prevalence of bovine trypanosomosis was determined from a total of 203 blood samples collected from Butaleja district, eastern Uganda. All samples were examined by microhematocrit centrifuge test (MHC), PCR and ELISA. ELISA was performed in accordance with the OIE standard procedures using Trypanosoma brucei gambiense procyclic form crude antigens. PCR were utilized to identify the species and the subspecies of trypanosome. The overall prevalence of bovine African trypanosomosis was 8.9% by MHC, and 45.3% by the ELISA. Since substantial number (12 out of 18) of MHC positive samples were negative in the PCR tests, we could not conclude the most epidemic trypanosome species in the studied area. Nevertheless, the PCR results suggests that the most prevalent...
Palavras-chave: Bovine; Prevalence; Trypanosome; Uganda; Zoonotic parasite.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2690
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Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for veccine against African Trypanosomosis OAK
Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文.
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML).
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222
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Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa OAK
NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant...
Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034
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Calcium ions are involved in egress of Babesia bovis merozoites from bovine erythrocytes OAK
MOSSAAD, Ehab; ASADA, Masahito; NAKATANI, Daichi; INOUE, Noboru; YOKOYAMA1), Naoaki; KANEKO, Osamu; KAWAZU, Shin-ichiro; 井上, 昇; 横山, 直明; 河津, 信一郎.
Bovine babesiosis is a livestock disease known to cause economic losses in endemic areas. The apicomplexan parasite Babesia bovis is able to invade and destroy the host’s erythrocytes leading to the serious pathologies of the disease, such as anemia and hemoglobinuria. Understanding the egress mechanisms of this parasite is therefore a key step to develop new therapeutic strategies. In this study, the possible involvement of Ca2+ in the egress of B. bovis merozoites from infected erythrocytes was investigated. Egress was artificially induced in vitro using calcium ionophore A23187 and thapsigargin to increase Ca2+ concentration in the cytosol of the parasite cells. The increased intracellular Ca2+ concentration following these treatments was confirmed...
Palavras-chave: Babesia bovis; Calcium ionophore; Calcium signalling; Egress.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4071
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CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS OAK
Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624
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Comparison of PCR with Parasitology and Serology in the Diagnosis of a Low Virulent Strain of Trypanosoma brucei gambiense in mice OAK
Mbati, P. A.; Hirumi, K.; Inoue, Noboru; Situakibanza, N. H.; Hirumi, Hiroyuki; 井上, 昇.
Palavras-chave: Trypanosoma brucei gambiense; Mice; Mel-B; Diagnosis; PCR.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/310
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Detection of Collagen-cross Reactive Antigenic Components in Toxoplasma gondii OAK
Omata, Yoshitaka; Inoue, Noboru; Yonematsu, Kunio; Claveria, Florencia G.; Igarashi, Ikuo; Saito, Atsushi; Suzuki, Naoyoshi; 井上, 昇; 五十嵐, 郁男.
Palavras-chave: Collagen; Cross-reactive antigens; Toxoplasma gondii.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/223
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Development of Monoclonal Antibodies That Target 1-Cys Peroxiredoxin and Differentiate Plasmodium falciparum from P. vivax and P. knowlesi OAK
Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎.
Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising...
Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859
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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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Different Activation of Lymphocytes in Reaction to Babesia microti Infection in iNOS-/- and Wildtype Mice OAK
Remer, Katharina; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行.
Palavras-chave: Lymphocytes; Babesia microti; Spleen; INOS-/- mice; CD4+ cells; CD8+ cells; IFN-γ; Antibody-titer.
Ano: 1997 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/263
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Establishment of an in vitro Transgene Expression System in Epimastigotes of Trypanosoma congolense OAK
Sakurai, Tatsuya; Tanaka, Miho; Shin-ichiro, Kawazu; Inoue, Noboru; 井上, 昇.
The epimastigote form (EMF) of Trypanosoma congolense appears in the late tsetse infective stage. Epimastigotes adhere to the tsetse proboscis, proliferate in this region, and differentiate into mammal-infective metacyclic forms (MCFs). This differentiation is called metacyclogenesis and is indispensable for cyclical transmission of the parasite. Although an in vitro culture method reproducing metacyclogenesis was established several decades ago, few genetic tools have been utilized to elucidate the molecular mechanisms underlying T. congolense metacyclogenesis. In this study, we established a transgene expression system in the EMF of T. congolense IL3000; the EMF was successfully cultured and observed to undergo metacyclogenesis in vitro. The newly...
Palavras-chave: Trypanosoma congolense; Epimastigotes; In vitro metacyclogenesis; Transfection; Transgene expression.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2677
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Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for Trypanosoma congolense OAK
SUGANUMA, Keisuke; ALLAMANDA, Puttik; HAKIMI, Hassan; ZHOU, Mo; ANGELES, Jose Ma.; KAWAZU, Shin-ichiro; INOUE, Noboru; 河津, 信一郎; 井上, 昇.
Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well...
Palavras-chave: Animal African trypanosomosis; Drug screening; Luciferase assay; Trypanosoma congolense.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3984
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First detection of Babesia venatorum (EU1) in Ixodes persulcatus ticks in Mongolia OAK
Tuvshintulga, B.; Battsetseg, B.; Battur, B.; Myagmarsuren, P.; Narantsatsral, S.; Sivakumar, T.; Takemae, H.; Igarashi, I.; Inoue, N.; Yokoyama, N.; 五十嵐, 郁男; 井上, 昇; 横山, 直明.
Babesia venatorum, formerly known as Babesia sp. EU1, is a zoonotic hemoprotozoan parasites that commonly infects deer. In the present study, we investigated B. venatorum infection in Ixodes persulcatus, an important tick vector capable of transmitting several tick-borne pathogens that cause babesiosis, encephalitis, tularemia, and Lyme diseases. DNA samples extracted from questing I. persulcatus ticks (n=63) that had been collected in Selenge province of Mongolia in 2012 and 2013 were screened for B. venatorum using a nested PCR assay. The findings showed that two of 63 DNA samples were positive for B. venatorum. The 18S rRNA sequences amplified from B. venatorum-positive DNA samples shared high identity scores (96.1–99.9%) with known B. venatorum...
Palavras-chave: Babesia venatorum; 18S rRNA; Ixodes persulcatus; Mongolia.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4243
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Identification and Characterization of a Trypanosoma congolense 46 kDa Protein as a Candidate Serodiagnostic Antigen OAK
ZHOU, Mo; SUGANUMA, Keisuke; RUTTAYAPORN, Ngasaman; NGUYEN, Thu-Thuy; YAMASAKI, Shino; IGARASHI, Ikuo; KAWAZU, Shin-ichiro; SUZUKI, Yasuhiko; INOUE, Noboru; 五十嵐, 郁男; 井上, 昇.
Trypanosoma congolense is a major livestock pathogen in Africa, causing large economic losses with serious effects on animal health. Reliable serodiagnostic tests are therefore urgently needed to control T. congolense infection. In this study, we have identified one T. congolense protein as a new candidate serodiagnostic antigen. The 46.4 kDa protein (TcP46, Gene ID: TcIL3000.0.25950) is expressed 5.36 times higher in metacyclic forms than epimastigote forms. The complete nucleotide sequences of TcP46 contained an open reading frame of 1,218 bp. Southern blot analysis indicated that at least two copies of the TcP46 gene were tandemly-arranged in the T. congolense genome. The recombinant TcP46 (rTcP46) was expressed in Escherichia coli as a glutathione...
Palavras-chave: Diagnosis; ELISA; Nagana; TcP46; Trypanosoma.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3983
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Non-invasive Method of Identification of SAG-1 Transgenic Mice by PCR Analysis of Oral Wash Cells OAK
Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南.
Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311
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PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam OAK
SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; YOSHINARI, Takeshi; TATTIYAPONG, Muncharee; GUSWANTO, Azirwan; OKUBO, Kazuhiro; IGARASHI, Ikuo; INOUE, Noboru; XUAN, Xuenan; YOKOYAMA, Naoaki; 井上, 昇; 玄, 学南; 横山, 直明.
Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue and Hanoi provinces of Vietnam. With the exception of T. annulata and T. evansi, all other parasite species (B. bovis, B. bigemina, T. orientalis and T. theileri) were detected in the cattle populations with B. bovis being the most common among them. Additionally, four water buffaloes and a single goat were infected with B. bovis and B. bigemina, respectively. The Hue...
Palavras-chave: Epidemiology; Hemoprotozoa; Livestock; PCR; Vietnam.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4015
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Preliminary studies for development of an immunochromatographic test (ICT) for detection of antibody against salivarian trypanosomes by using recombinant ribosomal P0 protein OAK
Cheng, Z; Goto, Y; Nguyen, T; Sakurai, T; Zang, J; Kawazu, S; Inoue, N; 河津, 信一郎; 井上, 昇.
http://www.obihiro.ac.jp/~protozoa/index.html
Palavras-chave: Trypanosomosis; Serodiagnosis; Immunechromatography.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3540
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Preliminary studies on the effects of orally-administered Transforming Growth Factor-beta on protozoan diseases in mice OAK
Namangala, Boniface; Inoue, Noboru; Sugimoto, Chihiro; 井上, 昇.
Transforming growth factor beta-1 (TGF-β1) is a pleiotropic cytokine with both pro- and antiinflammatory properties, depending on its environment and concentration. The present study evaluated the effects of orally-delivered TGF-β1 on mice parenterally-infected with various protozoan parasites. We report that while orally-administered TGF-β1 seems to confer partial protection against murine chronic babesiosis and acute trypanosomosis, no beneficial clinical effects were observed against acute babesiosis, malaria or toxoplasmosis. Taken together, these preliminary data suggest that the systemic effects conferred by exogenous TGF-β1 could be parasite speciesspecific. The variations in different parasitic infections could be due to (i) intrinsic differences...
Palavras-chave: Low-dose; Mice; Orally-delivered TGF-β1; Protection; Protozoan parasites.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2716
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Production of Transgenic Mice Carrying p30 Gene Encoding Major Surface Antigen of Toxoplasma gondii OAK
Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Inoue, Noboru; Igarashi, Ikuo; Nagasawa, Hideyuki; Horiuchi, Motohiro; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 五十嵐, 郁男; 長澤, 秀行.
Palavras-chave: Toxoplasma gondii; SAG-1 (p30); Transgenic mice.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/254
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Stability of Loop-Mediated Isothermal Amplification (LAMP) Reagents and its Amplification Efficiency on Crude Trypanosome DNA Templates OAK
THEKISOE, Oriel M. M; BAZIE, Raoul S. B; CORONEL-SERVIAN, Andrea M; SUGIMOTO, Chihiro; KAWAZU, Shin-ichiro; INOUE, Noboru; 井上, 昇.
This study evaluated the stability of LAMP reagents when stored at 25C and 37C, and also assessed its detection efficiency on different DNA template preparations. Accordingly, LAMP using reagents stored at 25C and 37C amplified DNA of in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to day 15 of reagent storage. There were no significant differences (P>0.05) in detection sensitivity of LAMP among the reagents stored at 25C, 37C and –20C (recommended storage temperature). LAMP using the reagents stored at above-mentioned temperatures amplified serially diluted DNAs (genomic DNA extracted by phenol-chloroform method, FTA card and hemolysed blood) of T. b. gambiense (IL2343) with high sensitivity. Reactions were conducted on the reagents stored...
Palavras-chave: Diagnosis; DNA template; LAMP; Parasitic disease; Trypanosoma.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2691
Registros recuperados: 28
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