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Registros recuperados: 25
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A preliminary study on origin of Callosciurus squirrels introduced into Japan OAK
Oshida, Tatsuo; Torii, Harumi; Lin, Liang-Kong; Lee, Jen-Kai; Chen, Yean-Jen; Endo, Hideki; Sasaki, Motoki; 押田, 龍夫; 佐々木, 基樹.
To examine the origin of Callosciurus erythraeus introduced to Japan, we compared mitochondrial DNA control region sequences of eight haplotypes from Japan with those of 42 haplotypes from Taiwan. There were two distinct phylogroups in Japan. Six haplotypes from Japan were included in the Taiwan population, suggesting that they could have been introduced from Taiwan. Two haplotypes, however, were distantly related to the cluster consisting of all Taiwanese haplotypes and six Japanese haplotypes. The uncorrected genetic distances (5.2-5.7%) between these two haplotypes and the out-group (Callosciurus finlaysonii from Laos) were less than those (8.3-9.8%) between these two haplotypes and the cluster consisting of the other six Japanese haplotypes and all...
Palavras-chave: Alien squirrel; Callosciurus erythraeus; Callosciurus finlaysonii; Mitochondrial DNA control region; Taiwan.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3039
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Accumulation of Eosinophils in the Infundibulum of the Bovine Oviduct Just after Ovulation OAK
KOWSAR, Rasoul; JIEMTAWEEBOON, Sineenard; SHIRASUNA, Koumei; SHIMIZU, Takashi; SASAKI, Motoki; KITAMURA, Nobuo; MIYAMOTO, Akio; 清水, 隆; 佐々木, 基樹; 宮本, 明夫.
This study was to investigate the localization and distribution of eosinophils (EOS) in the bovine oviduct throughout the estrous cycle. Histological studies revealed more abundant EOS in the infundibula of the oviducts ipsilateral to the preovulatory dominant follicle and the ovulated ovary. The number of EOS was higher in the infundibula of the oviducts ipsilateral to the ovulated ovary than those of the oviducts contralateral to the ovulated ovary. The infundibula of the oviducts ipsilateral to the preovulatory dominant follicle had higher number of EOS than those of the oviducts ipsilateral to the mid-cycle corpus luteum. The number of EOS in the isthmus, but not in the ampulla, was higher in the outer layers (tunica muscularis and tunica serosa) than...
Palavras-chave: Bovine; Eosinophil; Oviduct; Ovulation.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3947
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An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro OAK
LIU, Jinghui; A. MAREY, Mohamed; KOWSAR, Rasoul; HAMBRUCH, Nina; SHIMIZU, Takashi; HANEDA, Shingo; MATSUI, Motozumi; SASAKI, Motoki; HAYAKAWA, Hiroyuki; PFARRER, Christiane; MIYAMOTO, Akio; 羽田, 真悟; 松井, 基純; 佐々木, 基樹; 宮本, 明夫.
We have previously shown that polymorphonuclear neutrophils (PMNs) are present in bovine oviduct fluid under physiological conditions, and that the oviduct provides a microenvironment that protects sperm from phagocytosis by PMNs. Alpha 1-acid glycoprotein (AGP) is a major acute-phase protein produced mainly in the liver that has immunomodulatory functions. AGP mRNA is expressed in extrahepatic organs, such as the lung, kidney, spleen, lymph node, uterus, and ovary. Therefore, in this study, we investigated, 1) the local production of AGP in the bovine oviduct, 2) the effect of AGP on the phagocytic activity of PMNs for sperm and superoxide production and 3) the impact of AGP desialylation on the PMN phagocytosis of sperm. The AGP gene was expressed in...
Palavras-chave: Alpha 1-acid glycoprotein (AGP); Bovine; Oviduct; Phagocytosis; Sperm.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3946
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Areolae of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis) OAK
SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫.
In this study, we examined the existence and structure of areolae and the steroidogenesis of areolar trophoblast cells in the Antarctic minke whale placenta morphologically and immunohistochemically. Placentas were collected from the 15th, 16th and 18th Japanese Whale Research Program under Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. The opening and cavity of fetal areolae formed by taller columnar trophoblast cells (areolar trophoblast cells) with long microvilli and a bright cytoplasm, as compared with the trophoblast cells of the chorionic villi interdigitating with the endometrial crypts, were recognized in observations of serial sections. The opening of the areolar cavity...
Palavras-chave: Antarctic minke whale; Areola; Balaenoptera bonaerensis; Placenta; Steroidogenesis.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3861
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Classification of the seminiferous epithelial cycle in the sika deer (Cervus nippon) OAK
Hayakawa, Daisuke; Sasaki, Motoki; Suzuki, Masatsugu; Igota, Hiromasa; Kitamura, Nobuo; 佐々木, 基樹; 北村, 延夫.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4131
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Comparative Studies on lipid Analysis and Ultrastructure in Porcine and Southern Minke Whale (Balaenoptera Bonaerensis) Oocytes OAK
FUJlHIRA, Takuma; KINOSHITA, Mikio; SASAKI, Motoki; OHNISHI, Masao; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 木下, 幹朗; 佐々木, 基樹; 大西, 正男.
The present study was conducted to clarify the difference in the color of the cytoplasm in immature follicular oocytes from prepubertal and adult minke whales. The four lipid contents (triglyceride,total cholesterol phospholipids and non-esterified fatty acids) in vitrified immature oocytes from prepubertal and adult minke whales,and also in fresh and vitrified immature porcine oocytes,were measured. The lipid contents in vitrified-warmed minke whale oocytes were similarly high compared with those in vitrified-warmedp orcine oocytes. In particular,the total cholesterol and phospholipid contents in the vitrified immature oocytes from prepubertal and adult minke whales were significantly( P<0.05) higher than those from prepubertal pigs. Furthermore,the...
Palavras-chave: Minke whale; Porcine; Oocytes; Lipid; Sexual maturity.
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2731
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Distribution of Arteriolovenous Vessels, Capillaries and eNOS Expression in the Bovine Corpus Luteum During the Estrous Cycle : a Possible Implication of Different Sensitivity by Luteal Phase to PGF2α in the Increase of Luteal Blood Flow OAK
SHIRASUNA, Koumei; ASAHI, Takayuki; SASAKI, Motoki; SHIMIZU, Takashi; MIYAMOTO, Akio; 白砂, 孔明; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
We have shown that luteal blood flow increases in the peripheral vasculature of the mature corpus luteum (CL) prior to the onset of luteolysis in response to prostaglandin F2α (PGF2α) in the cow, but this phenomenon does not occur in the early CL. We therefore hypothesize that this acute increase of luteal blood flow occurs by vasodilation of large blood vessels due to local release of nitric oxide (NO). In the present study, we characterized the CL vasculature together with endothelial NO synthase (eNOS) expression during the estrous cycle in the cow. Immunohistochemistry was used to quantify the number of arteriolovenous vessels (surrounded with smooth muscle actin-positive smooth muscle cells), capillaries (with von Willebrand Factor-positive...
Palavras-chave: Blood vessels; Corpus luteum; ENOS; Luteolysis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2989
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Endothelin-1 downregulates sperm phagocytosis by neutrophils in vitro: a physiological implication in bovine oviduct immunity OAK
MAREY, Mohamed Ali; YOUSEF, Mohamed Samy; LIU, Jinghui; MORITA, Kazuhiro; SASAKI, Motoki; HAYAKAWA, Hiroyuki; SHIMIZU, Takashi; ELSHAHAWY, Ibrahim I.; MIYAMOTO, Akio; 佐々木, 基樹; 清水, 隆; 宮本, 明夫.
The oviduct is an active contractile tube that provides the proper environment for sperm transport, capacitation and survival. Oviductal contractions are regulated by autocrine/paracrine secretion of several factors, such as prostaglandins (PGs) and endothelin-1 (EDN-1). We have previously shown that during the preovulatory stage, sperm are exposed to polymorphonuclear neutrophils (PMNs) in the bovine oviduct, and the bovine oviduct epithelial cells (BOECs) secrete molecules including PGE2 that suppress sperm phagocytosis by PMNs in vitro. In this study, we investigated the possible effects of EDN-1 on the phagocytic activity of PMNs toward sperm. The local concentrations of EDN-1 in oviduct fluid and BOEC culture medium ranged from 10–10 to 10–11 M as...
Palavras-chave: Endothelin-1; Oviduct; Phagocytosis; Polymorphonuclear neutrophils (PMN); Sperm.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4310
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Humeral chondrosarcoma in a Hokkaido brown bear (Ursus arctos yesoensis). OAK
MURAKAMI, Tomoaki; KOBAYASHI, Yoshiyasu; CHIBA, Shiori; KURAUCHI, Yuki; SAKAMOTO, Hideyuki; SASAKI, Motoki; MATSUI, Takane; 古林, 与志安; 佐々木, 基樹.
Humeral chondrosarcoma was found in an 18-year-old male Hokkaido brown bear (Ursus arctos yesoensis). Necropsy revealed a large firm mass under the left superficial pectoral muscle at the axillary region. The mass involved the left shoulder joint and peripheral muscles, and connected to the head of the humerus with osteolysis. Histopathologically, the mass was composed of irregularly shaped myxomatous to cartilaginous tumor lobules. The tumor cell showed moderate nuclear atypia with a relatively high mitotic index, especially in the edges of the myxomatous lobules. The tumor cells were positively immunostained with vimentin and S-100 protein. Based on these findings, the tumors were diagnosed as chondrosarcoma. Metastases were found in the left axillary...
Palavras-chave: Brown bear; Chondrosarcoma; Humerus; Metastasis.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4122
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Immunohistochemical Localization of Steroidogenic Enzymes in the Testis of the Sika Deer (Cervus nippon) During Developmental and Seasonal Changes OAK
HAYAKAWA, Daisuke; SASAKI, Motoki; SUZUKI, Masatsugu; TSUBOTA, Toshio; IGOTA, Hiromasa; KAJI, Koichi; KITAMURA, Nobuo; 佐々木, 基樹; 北村, 延夫.
Testicular steroidogenesis and spermatogenesis during developmental and seasonal changes were investigated in male sika deer (Cervus nippon), a short-day seasonal breeder, to clarify the physiological mechanisms for reproductive function. The immunohistochemical localization of steroidogenic enzymes (P450scc, P450c17, 3βHSD and P450arom), spermatogenesis and cell proliferation were analyzed in the testes of fetal (164 to 218 days of fetal age), fawn (0 years old), yearling (1 year old) and adult (more than 2 years old) male sika deer. Three kinds of steroidogenic enzymes, P450scc, P450c17 and 3βHSD, essential for the synthesis of testosterone were located only in the Leydig cells of the testes from the fetal period, and these localizations did not change...
Palavras-chave: Developmental change; Seasonal change; Sika deer; Steroidogenic enzyme; Testis.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2816
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Immunohistochemical localization of the cytoskeletal proteins in the testes of the lesser mouse deer (Tragulus javanicus) OAK
Sasaki, Motoki; Endo, Hideki; Kimura, Junpei; Rerkamnuaychoke, Worawut; Hayakawa, Daisuke; Bhuminand, Devkota; Kitamura, Nobuo; Fukuta, Katsuhiro; 佐々木, 基樹; 北村, 延夫.
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4132
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Mitochondrial DNA Evidence Suggests Challenge to the Conspecific Status of the Hairy-Footed Flying Squirrel Belomys pearsonii from Taiwan and Vietnam OAK
Oshida, Tatsuo; Lin, Liang-Kong; Chang, Shih-Wei; Dang, Can Ngoc; Nguyen, Son Truong; Nguyen, Nghia Xuan; Nguyen, Dang Xuan; Endo, Hideki; Kimura, Junpei; Sasaki, Motoki; Hayashida, Akiko; Takano, Ai; 押田, 龍夫; 佐々木, 基樹.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4247
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Mobility of the forearm in the raccoon (Procyon lotor), raccoon dog (Nyctereutes procyonoides) and red panda (Ailurus fulgens) OAK
KAMIOKA, Minao; SASAKI, Motoki; YAMADA, Kazutaka; ENDO, Hideki; OISHI, Motoharu; YUHARA, Kazutoshi; TOMIKAWA, Sohei; SUGIMOTO, Miki; OSHIDA, Tatsuo; KONDOH, Daisuke; KITAMURA, Nobuo; 佐々木, 基樹; 押田, 龍夫; 近藤, 大輔; 北村, 延夫.
The ranges of pronation/supination of forearms in raccoons, raccoon dogs and red pandas were nondestructively examined. Three carcasses of each species were used for CT analysis, and the left forearms were scanned with a CT scanner in two positions: maximal supination and maximal pronation. Scanning data were reconstructed into three-dimensional images, cross-sectional images were extracted at the position that shows the largest area in the distal part of ulna, and then, the centroids of each cross section of the radius and ulna were detected. CT images of two positions were superimposed, by overlapping the outlines of each ulna, and then, the centroids were connected by lines to measure the angle of rotation, as an index of range of mobility. The...
Palavras-chave: Forearm; Raccoon; Raccoon dog; Red panda; Rotation.
Ano: 2017 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4394
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Phylogenetic Position of Callosciurus erythraeus griseimanus from Vietnam in the Genus Callosciurus full access OAK
Oshida, Tatsuo; Can Ngoc, Dang; Son Truong, Nguyen; Nghia Xuan, Nguyen; Endo, Hideki; Kimura, Junpei; Sasaki, Motoki; Hayashida, Akiko; Takano, Ai; Koyabu, Daisuke; Hayash, Yoshihiro; 押田, 龍夫; 佐々木, 基樹.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4112
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Preliminary Experiment on the Suppression of Cesium Uptake into the Organs by Bentonite Using a Stable Isotope of Cesium in Calves OAK
山田, 一孝; 山口, 敏朗; 陳, 忠正; 稲沢, 直生実; 石井, 三都夫; 佐々木, 基樹; 木田, 克弥; 岸本, 海織; 古濱, 和久; YAMADA, Kazutaka; ISHII, Mitsuo; SASAKI, Naoki; KIDA, Katsuya.
ベントナイトのセシウム体内移行抑制効果について調べた。実験には,ホルスタイン種子牛6頭を使用し,28日の実験期間中,全頭に安定同位体の塩化セシウム(3.0mg/kg/day)を人工乳に溶解し,投与した。塩化セシウムに加えベントナイト0.5w/v%を人工乳に懸濁した投与群(n=3)と塩化セシウムのみの対照群(n=3)の2群を設定した。実験開始0,1,2,3,7,14,21および28日目に採血,28日目に骨格筋,心臓,肝臓,腎臓および糞便を採材し,ICP-MS (inductively coupled plasma mass spectrometry) によるセシウム濃度を測定した。臓器中のセシウム濃度からベントナイトの効果は明らかではなかったが,血球中セシウム濃度平均値は投与後14日まで投与群が対照群よりも低値を示した。このことから,ベントナイトによるセシウム体内移行抑制効果の可能性が示唆された。
Palavras-chave: ベントナイト; 子牛; 塩化セシウム.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3898
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Structure and functions of the placenta in common minke (Balaenoptera acutorostrata), Bryde’s (B. brydei) and sei (B. borealis) whales OAK
KITAYAMA, Chiyo; SASAKI, Motoki; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; FUKUI, Yutaka; BUDIPITOJO, Teguh; KONDOH, Daisuke; KITAMURA, Nobuo; 佐々木, 基樹; 近藤, 大輔; 北村, 延夫.
The structure and functions of placentas were examined in 3 species of rorqual whales, common minke (Balaenoptera acutorostrata), Bryde’s (B. brydei) and sei (B. borealis) whales, with the aim of confirming the structural characteristics of the chorion, including the presence of the areolar part, and clarifying steroidogenic activities and fetomaternal interactions in the placentas of these whales. Placentas were collected from the second phase of the Japanese Whale Research Program under Special Permit in the North Pacific (JARPN II). Histological and ultrastructural examinations revealed that these whale placentas were epitheliochorial placentas with the interdigitation of chorionic villi lined by monolayer uninucleate cells (trophoblast cells) and...
Palavras-chave: Areola; Aromatase; Placenta; Uteroferrin; Whale.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4178
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Structure and Steroidogenesis of the Placenta in the Antarctic Minke Whale (Balaenoptera bonaerensis OAK
SASAKI, Motoki; AMANO, Yoko; HAYAKAWA, Daisuke; TSUBOTA, Toshio; ISHIKAWA, Hajime; MOGOE, Toshihiro; OHSUMI, Seiji; TETSUKA, Masafumi; MIYAMOTO, Akio; FUKUI, Yutaka; BUDIPITOJO, Teguh; KITAMURA, Nobuo; 佐々木, 基樹; 手塚, 雅文; 宮本, 明夫; 北村, 延夫.
There are few reports describing the structure and function of the whale placenta with the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of the Antarctic minke whale were observed morphologically and immunohistochemically. Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial placenta. The chorion was interdigitated to the endometrium by primary, secondary and tertiary villi, which contained no specialized trophoblast...
Palavras-chave: Antarctic minke whale; Balaenoptera bonaerensis; Morphology; Placenta; Steroidogenesis.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3862
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The developmental plasticity of colocalization pattern of peptide YY and glucagon-like peptide-1 in the endocrine cells of bovine rectum OAK
Asadullah, Hamid,Pyarokhil; Ishihara, Miyuki; Sasaki, Motoki; Kitamura, Nobuo; 佐々木, 基樹; 北村, 延夫.
Peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) are produced in endocrine cells that show distal distribution in each of small and large intestine. They are colocalized in the same endocrine cells at different ratios depending on the animal species. The present study examined the possibility of plasticity in the colocalization pattern in the bovine rectum, which is known to contain endocrine cells at a high concentration. Consecutive sections from different pre- and postnatal stages were stained immunohistochemically. The immunoreactive (IR) cells were divided into three groups: 1) cells IR for both PYY and GLP-1 (PYY/GLP-1-IR cells), 2) cells IR only for PYY (PYY-IR cells), and 3) cells IR only for GLP-1 (GLP-1-IR cells). The percentage of...
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3854
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The Lamination of the Masseter Muscle in the Water Deer ( Hydropotes inermis ) OAK
Sasaki, Motoki; Kimura, Junpei; Sohn, Joonhyuk; Nasu, Tetsuo; Kitamura, Nobuo; Yasuda, Masahiro; 佐々木, 基樹; 北村, 延夫.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4133
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The origin and early evolution of lactation, a new hypothesis proposed by Olav T. Oftedal OAK
浦島, 匡; 上村, 祐介; 中村, 正; 佐々木, 基樹; Urashima, Tadasu; Uemura, Yuhsuke; Nakamura, Tadashi; Sasaki, Motoki.
ペンシルバニア紀(>3億1000万年前)に,有羊膜類であるSynapsidは他の系統から分化し,腺を発達させた。乳腺は毛包と会合した祖先型アポクリン腺から発達した。乳腺と毛の関係は単孔類の乳腺に保持されている。また,有袋類における乳腺毛の初期個体発生時の存在とその後の退化に,その証拠が残されている。単孔類の乳頭のない乳腺区分にあるmammo-ピロ-脂腺単位の密集した集団は,透水性を示す卵への水分や他の成分の補給のための構造的進化を反映しているかもしれない。ラクトースなどの成分は,初期Synapsidの祖先アポクリン腺から分泌されていたかもしれない。三畳紀のTherapsidは,複雑な栄養成分を含む乳を分泌し,卵サイズを小さくし,孵化時の新生子の未熟化を引き起こした。乳腺区分に生じた乳頭は,乳腺の毛を退化させた。 乳腺分泌は,羊皮状の卵殻をもつ卵を産むSynapsidで開始されたと考えられる。羊皮状の卵殻をもつ卵は,低湿度の空気にふれた際,急速に水分損失を被る。Synapsidは抱卵嚢の中で抱卵することで卵の乾燥を防いだが,これは母親の行動を制限した。羊皮状の卵殻をもつ卵は,殻を通して液体水分を補給することができる。乳腺分泌は,本来卵に水分を供給する手段として開始され,Cynodont (キノドン類)において内温性獲得の要因となった。Synapsidの卵は,ナトリウムやカルシウムイオンなどの栄養素を摂取した可能性がある。孔のある卵殻と2層性の卵黄膜は,子宮内での分泌物と抱卵時での乳腺分泌物の摂取を促進したと考えられる。
Ano: 2004 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2924
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