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11カ月齢の乳用育成午における腹腔内の巨大な膿瘍形成を伴った臍帯炎の1症例 OAK
真方, 文絵; 渡辺, 謙一; 下田, 崇; 古林, 与志安; 松井, 高峯; 石井, 三都夫; 猪熊, 壽.
11カ月齢のホルスタイン種乳牛が重度の発育不良を呈し、臍の腫脹が見られた。臨床症状および血液検査所 見より臍帯炎を疑った。超音波検査および穿刺により、臍の腫脹部と膀胱の腹側においてそれぞれ直径10cmお よび5Cmの膿瘍の存在を確認した。病理解剖により臍部、臍静脈、臍動脈および尿膜管相当部に膿瘍が認めら れ、中でも臍静脈相当部の膿瘍は50X40X15cmと巨大であった。育成期に発育不良を伴う症例では、慢性化し た臍帯炎も考慮する必要が考えられた。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2934
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A case of intra-abdominal mass associated with the urachal remnant in a Holstein bullock OAK
望月, 奈那子; 滄木, 孝弘; 伊藤, めぐみ; 藤田, 理公; 堀内, 雅之; 古林, 与志安; 猪熊, 壽; 芝野, 健一.
9カ月齢のホルスタイン去勢牛が食欲廃絶,乏尿および疝痛様症状を呈し,尿中からリン酸アンモニウムマグネシウムの結石が検出されたことから尿石症と診断された.対症療法を行ったところ,症状の増悪はないが食欲や全身状態の顕著な改善が得られないまま経過した.1カ月後,発熱,脱水症状,乏尿および腹囲膨満が認められ,尿路閉塞に伴う膀胱破裂が疑われたが,血清中クレアチニンおよび尿素窒素濃度の上昇など,膀胱破裂に特徴的な血液生化学的所見は認められなかった.確定診断に至らないまま予後不良と判断され,安楽殺された.病理解剖の結果,膀胱尖が小さく開口し,その先に腹腔の右側を占拠する巨大な腫瘤が認められた. 膀胱尖部の炎症性反応が乏しいことなどから,腫瘤のうち,膀胱と連絡する部分は遺残した尿膜管と診断された.また,腫瘤と周辺組織との癒着が広範囲にわたっていることから,尿膜管が何らかの原因により破綻し,尿が腹腔内に漏出したものの,大網に被覆された結果,腹膜炎が限局性にとどまり,巨大な嚢胞を形成するに至ったと推察された. 尿石症による尿路閉塞や膀胱破裂が疑われる症例において特徴的な血液生化学的所見が得られない場合は,月齢が進んだ個体であっても,尿膜管憩室やそれに起因する限局性の腹膜炎を類症鑑別に加える必要があると考えられた.
Palavras-chave: 腹腔内腫瘤; 尿膜管遺残; 尿膜管憩室; 限局性腹膜炎; 尿石症; Intra-abdominal mass; Urachal remnant; Urachal diverticulum; Localized peritonitis; Urolithiasis.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4522
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A Clinical Case of Maxillary Sinus Abscess in a Holstein Cow OAK
髙橋, 英二; 山田, 一孝; 古林, 与志安; 猪熊, 壽; TAKAHASHI, Eiji; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
7歳6カ月齢のホルスタイン種乳牛が顕著ないびき音を呈し,さらに左側頬骨部の一部で波動感を有する腫瘤が触知された.頭部X線検査により上顎洞内への液体貯留と上顎第3後臼歯の歯槽骨の融解が認められ,歯性上顎洞炎に継発する大型膿瘍であることが推察された.病理解剖により病変は上顎洞内膿瘍であることが明らかとなった.牛における頭部X線検査が上顎洞内膿瘍の診断及び病態の把握に有用であった.
Palavras-chave: ホルスタイン; 上顎洞内膿瘍; 歯性上顎洞炎.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4257
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A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' in Hokkaido, Japan OAK
TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan.
Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582
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A Mechanism of Excessive Accumulation of Abomasal Gas in Vagotomized Cattle Determined using Fluoroscopy OAK
ITOH, Megumi; SASAKI, Naoki; KAWAMOTO, Satoshi; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽.
To better understand the mechanism of excessive gas accumulation in the abomasum in bovine abomasal displacement, we performed gastric fluoroscopy in vagotomized cattle. Fifteen 6-month-old Holstein steers were divided into three groups: a non-vagotomized control group (Group C; n=5), a ventral thoraco-vagotomized group (Group V; n=5), and a dorsal and ventral thoraco-vagotomized group (Group DV; n=5). These groups were examined by fluoroscopy before and during a 5-week observation period after surgery. In Group C, no change was observed throughout the observation period. In Group DV, immediately after surgery, reticuloruminal motility was completely absent and ruminal distention was seen. Two weeks after surgery, abnormal reticulum motility and increased...
Palavras-chave: Abomasum gas; Cattle; Displaced abomasum; Vagotomy.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3888
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A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries OAK
SIVAKUMAR, Thillaiampalam; ASADA, Masahito; BATTSETSEG, Badgar; HUANG, Xiaohong; LAN, Dinh Thi Bich; INPANKAEW, Tawin; P. YBAÑEZ, Adrian; ALHASSAN, Andy; THEKISOE, Oriel M. M.; DE MACEDO, Alan Caine Costa; INOKUMA, Hisashi; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 猪熊, 壽; 五十嵐, 郁男; 横山, 直明.
Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B....
Palavras-chave: AMA-1; Babesia ovata; Cattle; Mongolia; Thailand.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4016
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Anaplasma platys 感染症およびその他のアナプラズマ属感染症:小動物のリケッチア性疾患(2) OAK
猪熊, 壽.
Palavras-chave: 小動物; リケッチア性疾患; Anaplasma platys 感染症; アナプラズマ属感染症.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/990
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Astasia and pyrexia related to Borrelia garinii infection in two dogs in Hokkaido, Japan. OAK
INOKUMA, Hisashi; MAETANI, Shigeki; FUJITSUKA, Jyunji; TAKANO, Ai; SATO, Kozue; FUKUI, Takashi; MASUZAWA, Toshiyuki; KAWABATA, Hiroki; 猪熊, 壽.
Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs was Lyme disease related to B. garinii infection.
Palavras-chave: Borrelia garinii; Canine; Japan; Lyme disease.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4099
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Brain Abscess in a Japanese Black Calf : Utility of Computed Tomography (CT) OAK
EL-KHODERY, Sabry; YAMADA, Kazutaka; AOKI, Daisuke; KAMIO, Kyouhei; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; ISHII, Mitsuo; INOKUMA, Hisashi; YAMAUCHI, Sen-ichi; MATSUI, Takane; 山田, 一孝; 古林, 与志安; 石井, 三都夫; 猪熊, 壽.
Computed tomography (CT) was used for diagnosis of brain abscess in a 6-month-old, Japanese black calf presented with neurological dysfunction, compulsive circling and vision disturbance. CT images showed asymmetric lateral ventricles, and presence of intra-cranial multiple low absorption lesions surrounded by capsule suggestive of abscess in the right cerebral hemisphere. Postmortem examination revealed marked swelling of right cerebral hemisphere and olfactory bulb. Multilocular large abscess containing creamy pus was found to occupy most area of periventricular and lateral ventricle. Fusobacterium necrophrum was isolated from the abscess contents as the causative agent. These results demonstrate that CT is useful tool for tentative diagnosis of bovine...
Palavras-chave: Bovine; Brain abscess; CT.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3926
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Calf Form Bovine Leukosis with Lameness in a Holstein Heifer OAK
TAWFEEQ, Mohammad Monir; MIURA, Saori; NAKANISHI, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽.
A 12-month-old Holstein heifer with anorexia, lameness, and enlargement of peripheral lymph nodes was suspected of having bovine leukosis. Although lymphocytosis was not observed, cytology of fine needle aspirate from a superficial cervical node, and increased serum lactate dehydrogenase and thymidine kinase activities, strongly suggested lymphosarcoma. Increased numbers of mononuclear cells as well as mitotic cells were observed in synovial fluid collected from swollen joints. Pathological examination confirmed B-cell calf form bovine leukosis and joint swelling related to neoplastic cell infiltration. Both interleukin-2 receptor and thymidine kinase 1 genes were highly expressed in cells from superficial cervical lymph node aspirate.
Palavras-chave: Calf form bovine leukosis; Gene expression; Joint infiltration.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4097
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Cerebellar Cortical Degeneration in a Japanese Black Bull OAK
田川, 道人; 千葉, 史織; 岡松, 弘之; 小嶋, 由夏; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
黒毛和種牛が出生翌日より横臥,後弓反張を呈した.脳挫傷を疑い,抗生物質,ビタミンB1製剤,消炎鎮痛剤により加療され,いったんは改善したが同様の症状が間欠的に認められた.歩様異常は継続して認められたが,一般状態は良好で8カ月齢まで飼養された.しかし,その後開脚姿勢,測定過大を伴う歩様異常,威嚇瞬き反応の低下が進行した.病理組織学的検索により小脳にプルキンエ細胞の顕著な変性と脱落及び顆粒細胞の減数を認め,小脳皮質変性症と診断された.
Palavras-chave: ; 小脳皮質変性症; 黒毛和種.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4101
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Chronic interstitial nephritis with edema in a Holstein heifer OAK
髙橋, 英二; 竹内, 俊彦; 丸山, 亮介; 古林, 与志安; 猪熊, 壽; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
1歳4カ月齢のホルスタイン種育成牛が発育不良を主訴に受診した.当該牛は体高110cmで,著しい削痩を呈していた.臨床検査により,非再生性貧血,高窒素血症,高クレアチニン血症,高リン血症,低カルシウム血症,低比重尿および蛋白尿が認められ,慢性腎不全が疑われた.輸液により治療を試みたが,第15病日には下顎と胸垂の浮腫が発現し,第16病日に死亡した.病理学的検索の結果,糸球体病変を伴う慢性間質性腎炎と診断されたが,原因は不明であった.
Palavras-chave: 慢性間質性腎炎; 浮腫; ホルスタイン育成牛.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4366
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Clinical Case of a Brainstem Tumor in a Holstein Cow OAK
三浦, 沙織; 千葉, 史織; 高橋, 裕之; 福田, 茂夫; 藤井, 貴志; 新井, 鐘蔵; 古林, 与志安; 猪熊, 壽; MIURA, Saori; CHIBA, Shiori; TAKAHASHI, Hiroyuki; FUKUDA, Shigeo; FUJII, Takashi; ARAI, Shozo; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
38カ月齢のホルスタイン種乳牛において,体全体が左側に傾くという神経症状が進行性に認められた.経過,左側対光反射と左側音響耳介反応の減弱,及び聴性脳幹誘発電位測定検査における左刺激時Ⅲ波-Ⅴ波の遅延所見より,延髄から橋における片側性圧迫性病変を疑った.病理解剖の結果,橋左側に帯黄色脆弱化病巣を認め,病理組織学的検索により,病変は腫瘍であり,迷入組織に由来する可能性があるものの,原発組織は確定できなかった.
Palavras-chave: 聴性脳幹誘発電位; 脳幹; 腫瘍; ホルスタイン.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4010
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Clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 OAK
Miyama, Takako; Inokuma, Hisashi; Itamoto, Kazuhito; Okuda, Masaru; Verdida, Rodolfo A.; Xuan, Xuenan; 猪熊, 壽; 玄, 学南.
The clinical usefulness of antibodies against Babesia gibsoni detected by ELISA with recombinant P50 was examined in dogs in an area where B. gibsoni infection was endemic. Only 8 among 14 dogs with acute type B. gibsoni infection without a previous history of infection were positive. This high percentage of false-negative results is thought to be a weak point of ELISA as a diagnostic tool. However, 14 other anemic dogs with a confirmed history of B. gibsoni infection were all positive, thus confirming the higher sensitivity of ELISA in detecting a history of infection.
Palavras-chave: Babesia gibsoni; Diagnosis; ELISA.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/925
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Comparison of Bacterial Endotoxin Lipopolysaccharide Concentrations in the Blood, Ovarian Follicular Fluid and Uterine Fluid: A Clinical Case of Bovine Metritis OAK
MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆.
We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis.
Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043
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Congestive heart failure caused by an arteriovenous fistula between the brachiocephalic trunk and the vena cava cranialis in a Holstein cow OAK
嘉陽, 静香; 二宮, 理紗; 古関, 博; 堀内, 雅之; 古林, 与志安; 猪熊, 壽; Horiuchi, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
2歳6カ月齢のホルスタイン種成乳牛が水様性下痢を主訴に受診した.第8病日には,胸垂の冷性浮腫および頸静脈怒張が発現し,収縮期駆出性雑音が聴取された.血液検査,心電図検査および心臓超音波検査を実施したが,生前にうっ血性心不全の原因は特定できなかった.病理解剖により,腕頭動脈と前大静脈の短絡が確認された.また,前縦隔において前大静脈に大型の静脈瘤(直径10cm)が形成されていた.本症例は,先天性動静脈瘻の結果,静脈瘤形成とうっ血性心不全を呈した成牛の稀な症例と思われた.
Palavras-chave: 先天性動静脈瘻; うっ血性心不全; 駆出性雑; 静脈瘤.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4146
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Detection of Anaplasma bovis DNA in the Peripheral Blood of Domestic Dogs in Japan OAK
Sakamoto, Leo; Ichikawa, Yasuaki; Sakata, Yoshimi; Matsumoto, Kotaro; Inokuma, Hisashi; 松本, 高太郎; 猪熊, 壽.
The prevalence of Ehrlichia and Anaplasma in 1,427 dogs from 32 Japanese prefectures was evaluated by PCR and DNA nucleotide sequencing. PCR screening demonstrated that 18 dogs (1.3%) were positive for Anaplasmataceae. Sequence analysis revealed that 14 of the amplicons were most closely related to Wolbachia spp., symbionts of Dirofilaria immitis, whereas three were identified as Anaplasma bovis. The remaining amplicon could not be sequenced. Almost the entire sequence of 16S rRNA (1,452 bp) from one of the positive specimens was determined, and subsequent phylogenetic analysis confirmed that the detected sequence was that of A. bovis. This is the first detection of A. bovis DNA fragments in dogs. Species-specific nested PCR showed that 15 (1.1%) of the...
Ano: 2010 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3083
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Detection of Babesia canis rossi, B-canis vogeli, and Hepatozoon canis in dogs in a village of eastern Sudan by using a screening PCR and sequencing methodologies OAK
Oyamada, Maremichi; Davoust, Bernard; Boni, Mickaël; Dereure, Jacques; Bucheton, Bruno; Hammad, Awad; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
Babesia and Hepatozoon infections of dogs in a village of eastern Sudan were analyzed by using a single PCR and sequencing. Among 78 dogs, 5 were infected with Babesia canis rossi and 2 others were infected with B. canis vogeli. Thirty-three dogs were positive for Hepatozoon. Hepatozoon canis was detected by sequence analysis.
Palavras-chave: NORTH-AMERICA; JAPAN.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/80
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Detection of DNA of 'Candidatus Mycoplasma haemominutum' and Spiroplasma sp. in unfed ticks collected from vegetation in Japan OAK
Taroura, Shoko; Shimada, Yojiro; Sakata, Yoshimi; Miyama, Takako; Hiraoka, Hiroko; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA fragments of 'Candidatus Mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed Ixodes ovatus collected from vegetation in Hokkaido, Fukushima and Yamaguchi Prefectures, and unfed Haemaphysalis flava in Yamaguchi Prefecture. This finding suggests that ixodid tick is a possible vector of 'C. Mycoplasma haemominutum'. Spiroplasma DNA was also detected from unfed I. ovatus in Hokkaido, Fukushima and Yamaguchi Prefectures. The analysis of nucleotides sequence suggested that this Spiroplasma was distinct from registered species.
Palavras-chave: Candidatus Mycoplasma haemominutum; Spiroplasma sp; Tick.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1654
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Detection of Rickettsial DNA in ixodid ticks recovered from dogs and cats in Japan OAK
Hiraoka, Hiroko; Shimada, Yojiro; Sakata, Yoshimi; Watanabe, Malaika; Itamoto, Kazuhito; Okuda, Masaru; Inokuma, Hisashi; 猪熊, 壽.
DNA from ticks recovered from 1137 dogs and 133 cats from Ell over Japan were examined for Rickettsia infection by citrate synthase gene (gltA)-based PCR and partial nucleotide sequencing. A total of 91 dog tick samples and 18 cat tick samples showed a single band of the appropriate size in the nested PCR. Sequence analysis was successfully performed on 102 samples. DNA of Rickettsia japonica or closely related Rickettsia spp. strains were detected from 38 licks in 16 prefectures mainly in western Japan. The other 33, detected from 13 prefectures including Hokkaido and Okinawa, were found to be Rickettsia helvetica or closely related strains. A total of 29 DNA that showed highest homology with Rickettsia akari or closely related strains were detected in 19...
Palavras-chave: Canine; Epidemiology; Feline; Rickettsia; Tick.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/923
Registros recuperados: 159
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