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11カ月齢の乳用育成午における腹腔内の巨大な膿瘍形成を伴った臍帯炎の1症例 OAK
真方, 文絵; 渡辺, 謙一; 下田, 崇; 古林, 与志安; 松井, 高峯; 石井, 三都夫; 猪熊, 壽.
11カ月齢のホルスタイン種乳牛が重度の発育不良を呈し、臍の腫脹が見られた。臨床症状および血液検査所 見より臍帯炎を疑った。超音波検査および穿刺により、臍の腫脹部と膀胱の腹側においてそれぞれ直径10cmお よび5Cmの膿瘍の存在を確認した。病理解剖により臍部、臍静脈、臍動脈および尿膜管相当部に膿瘍が認めら れ、中でも臍静脈相当部の膿瘍は50X40X15cmと巨大であった。育成期に発育不良を伴う症例では、慢性化し た臍帯炎も考慮する必要が考えられた。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2934
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4種類の経口Ca製剤における乳牛の分娩性低Ca血症予防効果に関する検討 OAK
石井, 三都夫; Ishii, Mitsuo.
平成18年度 Ⅰ.基盤研究部門
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2589
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Brain Abscess in a Japanese Black Calf : Utility of Computed Tomography (CT) OAK
EL-KHODERY, Sabry; YAMADA, Kazutaka; AOKI, Daisuke; KAMIO, Kyouhei; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; ISHII, Mitsuo; INOKUMA, Hisashi; YAMAUCHI, Sen-ichi; MATSUI, Takane; 山田, 一孝; 古林, 与志安; 石井, 三都夫; 猪熊, 壽.
Computed tomography (CT) was used for diagnosis of brain abscess in a 6-month-old, Japanese black calf presented with neurological dysfunction, compulsive circling and vision disturbance. CT images showed asymmetric lateral ventricles, and presence of intra-cranial multiple low absorption lesions surrounded by capsule suggestive of abscess in the right cerebral hemisphere. Postmortem examination revealed marked swelling of right cerebral hemisphere and olfactory bulb. Multilocular large abscess containing creamy pus was found to occupy most area of periventricular and lateral ventricle. Fusobacterium necrophrum was isolated from the abscess contents as the causative agent. These results demonstrate that CT is useful tool for tentative diagnosis of bovine...
Palavras-chave: Bovine; Brain abscess; CT.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3926
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Diagnosis of failure of passive transfer in seven-day-old calves based on the serum IgG and total protein concentrations OAK
新盛, 英子; 滄木, 孝弘; 石井, 三都夫; Aoki, Takahiro; ISHII, Mitsuo.
1日齢における血中免疫グロブリンG(IgG)濃度が基準値未満の場合に受動免疫移行不全(FPT)と診断されるが,子牛が肥育牧場や育成牧場に導入される1週齢前後のFPT基準値は報告されていない. 本研究は, 血清IgGおよび総タンパク質(TP)濃度の経時的変化を調べ, 7日齢の検査値を用いたFPT診断精度について検証した. ホルスタイン種(HS)43頭, 黒毛和種(JB)34頭を用いた. 1日齢と7日齢に採血を行い, 血清IgG(mg/mℓ)およびTP濃度(g/dℓ)を測定した. 1日齢と7日齢において, IgGとTPの間に強い正の相関関係が認められた. 1日齢のIgGとTPの散布図において, 近似曲線の一次方程式にFPT基準値(HS-IgG: 10.0, JB-IgG: 20.0)を代入して求めたTP濃度(HS-TP: 4.6, JB-TP: 5.3)を1日齢のFPT基準値とした. HS-IgG, HS-TPおよびJB-IgGは, 1日齢〜7日齢にかけて有意に減少した. 1日齢〜7日齢の平均変化率は, HS-IgG: 71.7%, HS-TP: 92.2%, JB-IgG: 73.8%, JB-TP: 95.4%であった. 1日齢のFPT基準値に平均変化率を乗じて7日齢のFPT基準値とした(HS-IgG: 7.2, HS-TP: 4.2, JB-IgG: 14.8, JB-TP: 5.1). 7日齢の血液検査において, FPT基準値未満であった場合を検査陽性とし, FPTの診断精度について検証した. HS-IgGの陽性的中率(PPV)は78.6%, 陰性的中率(NPV)は93.3%, HS-TPのPPVは76.5%, NPVは100.0%であった. JB-IgGのPPVは100.0%, NPVは80.0%,...
Palavras-chave: 7日齢; 子牛; 受動免疫移行不全; IgG; TP.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3896
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Effect of Chronic Hemoplasma Infection on Cattle Productivity OAK
TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽.
The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower...
Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870
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Effect of Suckling on the Reproductive Performance and Metabolic Status of Obese Japanese Black Cattle during the Early Postpartum Period OAK
KAWASHIMA, Chiho; KIDA, Katsuya; MATSUHASHI, Miki; MATSUI, Motozumi; SHIMIZU, Takashi; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 川島, 千帆; 木田, 克弥; 松井, 基純; 清水, 隆; 石井, 三都夫; 三宅, 陽一; 宮本, 明夫; 松長, 延吉.
The aim of our study was to investigate the effects of suckling on reproductive performance and metabolic status of obese (mean body condition score of more than 4.0 on a scale of 1-5) maternal Japanese Black cows during early postpartum period. We used 7 postpartum Japanese Black cattle. Four cows were suckled ad libitum (suckled) until completion of their first artificial insemination (AI), while 3 cows were not suckled at all because they were separated from their calves immediately after parturition (non-suckled). Body weight and plasma concentrations of metabolites and hormones were measured from wk 1 to 9 postpartum. Ovarian activity was detected using plasma progesterone concentration, and all cows received their first AI after application of the...
Palavras-chave: Japanese Black cattle; Metabolic status; Obesity; Reproductive performance; Suckling.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2991
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Effects of the degree of traction during calving on viability, blood gas and serum IgG concentration in dairy calves. OAK
Sugimoto, H.; Aoki, Takahiro; Endo, H.; Yamakawa, K.; Shinmori, E.; Ishii, Mitsuo; 滄木, 孝弘; 石井, 三都夫.
道東の2農場にて出生したホルスタイン乳用子牛計36頭を用い,牽引の程度が子牛の活力,血液ガス,血清免疫グロブリン (Ig) G濃度に及ぼす影響を調査した.牽引状況を数値化するために,牽引強度と牽引時間をそれぞれ4段階 (0~3) で評価しスコア化したものを掛け合わせて牽引スコア (0~9) とし,牽引スコアの値によって子牛を自然分娩群(0),軽度牽引群(1),中程度牽引群(2~3)および重度牽引群(4~9)の4群に分類した.活力の評価には,ヒトのApgarスコアを子牛用に改変したものを用い,出生3分後に評価した.子牛の採血は頸静脈より行った.結果,Apgarスコアは自然分娩群と比較し軽度牽引群,重度牽引群で有意に低下した (それぞれp<0.05).出生5分後の静脈血水素イオン指数( pH) は,中程度から重度の牽引群において自然分娩群から軽度牽引群までと比較して有意に低下し (p<0.01),二酸化炭素分圧 (pCO2) は有意に増加した (p<0.005) が,重炭酸イオン濃度( HCO3−) はどの時点においても群間に差は認めなかった (p>0.75).また,出生24時間後の血清IgG濃度は自然分娩群と牽引群の間には有意な差は認めなかったが,牽引群内で重度になるにつれ低下し,軽度・重度牽引群間で有意差が認められた (p<0.05).さらに,出生5分後の静脈血pHと24時間後血清IgG濃度との間に有意な相関が認められた...
Palavras-chave: 牽引; 乳用新生子牛; Apgar; 血液ガス分析; IgG濃度.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3904
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Growth, hematologic and serum biochemical effects of hatakeshimeji (Lyophyllum decastes Sing.)extracts in calves OAK
稲沢, 直生実; 山川, 和宏; 石井, 三都夫; 李, 奇子; 室谷, 直義; 池水, 智博; 山田, 一孝; ISHII, Mitsuo; YAMADA, Kazutaka.
ハタケシメジ抽出物がホルスタイン種哺乳子牛の成長および血液学的検査値に与える影響について検討した.試験群は,ハタケシメジ抽出物投与群(雌,n=5)と対照群(雌,n=5)とし,投与群には朝晩の哺乳時にハタケシメジ抽出物(160mg)3カプセルを投与した.試験期間は,出生から8週間とした.試験期間中,投与群と対照群との間で増体に差が認められず,投与群の血液学的および血清生化学検査値は対照群と同様の変化を示した.このことから,ハタケシメジ抽出物は,哺乳子牛の健康状態,増体および血液学的値に悪影響を及ぼさないと考えられた.
Palavras-chave: 子牛 ハタケシメジ抽出物; 血液学的検査 血清生化学検査.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4207
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Peripartum Back Fat Thickness of Multiparous Holstein-Friesian Cows with Displacement of the Abomasum or Ketosis OAK
YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUI, Motozumi; YAMADA, Kazutaka; ISHII, Mitsuo; 滄木, 孝弘; 山田, 一孝; 石井, 三都夫.
To establish a method to predict postpartum diseases using prepartum back fat thickness (BFT), the peripartum BFTs of 54 healthy multiparous cows before calving, which were diagnosed with postpartum displacement of the abomasum (DA), clinical ketosis or subclinical ketosis were compared with those of healthy cows from 8 weeks before the expected calving date to 8 weeks after calving. The peripartum BFTs of the cows with DA or clinical ketosis were significantly higher than those of healthy cows. The peripartum BFTs of the cows with subclinical ketosis were not significantly higher than those of the healthy cows.
Palavras-chave: Back fat thickness (BFT); Clinical ketosis; Displacement of the abomasum (DA); Obese cow.
Ano: 2012 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3903
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Pilot Study of Bovine Interdigital Cassetteless Computed Radiography OAK
El-Sayed Ahmed Awad EL-SHAFAEY1) 2); AOKI, Takahiro; ISHII, Mitsuo; YAMADA, Kazutaka; 滄木, 孝弘; 石井, 三都夫; 山田, 一孝.
Twenty-one limbs of bovine cadavers (42 digits) were exposed to interdigital cassetteless imaging plate using computed radiography. The radiographic findings included exostosis, a rough planta surface, osteolysis of the apex of the distal phalanx and widening of the laminar zone between the distal phalanx and the hoof wall. All these findings were confirmed by computed tomography. The hindlimbs (19 digits) showed more changes than the forelimbs (10 digits), particularly in the lateral distal phalanx. The cassetteless computed radiography technique is expected to be an easily applicable method for the distal phalanx rather than a conventional cassette-plate and/or the film-screen cassetteless methods.
Palavras-chave: Bovine; Cassetteless; Computed radiography; Interdigital.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3899
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Preliminary Experiment on the Suppression of Cesium Uptake into the Organs by Bentonite Using a Stable Isotope of Cesium in Calves OAK
山田, 一孝; 山口, 敏朗; 陳, 忠正; 稲沢, 直生実; 石井, 三都夫; 佐々木, 基樹; 木田, 克弥; 岸本, 海織; 古濱, 和久; YAMADA, Kazutaka; ISHII, Mitsuo; SASAKI, Naoki; KIDA, Katsuya.
ベントナイトのセシウム体内移行抑制効果について調べた。実験には,ホルスタイン種子牛6頭を使用し,28日の実験期間中,全頭に安定同位体の塩化セシウム(3.0mg/kg/day)を人工乳に溶解し,投与した。塩化セシウムに加えベントナイト0.5w/v%を人工乳に懸濁した投与群(n=3)と塩化セシウムのみの対照群(n=3)の2群を設定した。実験開始0,1,2,3,7,14,21および28日目に採血,28日目に骨格筋,心臓,肝臓,腎臓および糞便を採材し,ICP-MS (inductively coupled plasma mass spectrometry) によるセシウム濃度を測定した。臓器中のセシウム濃度からベントナイトの効果は明らかではなかったが,血球中セシウム濃度平均値は投与後14日まで投与群が対照群よりも低値を示した。このことから,ベントナイトによるセシウム体内移行抑制効果の可能性が示唆された。
Palavras-chave: ベントナイト; 子牛; 塩化セシウム.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3898
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Relationship between Peripartal Plasma Oxytocin and Prostaglandin F2α Metabolite and Placental Expulsion Time in Heavy Draft Mares OAK
ISHII, Mitsuo; KOBAYASHI, Syuichi; ACOSTA, Tomas J.; MIKI, Wataru; YAMANOI, Takahiro; MATSUI, Motozumi; MIYAKE, Yoh-Ichi; MIYAMOTO, Akio; 石井, 三都夫; 松井, 基純; 三宅, 陽一; 宮本, 明夫.
The aim of this study was to clarify the relationship between circulating oxytocin (OT) and PGF2α metabolite (PGFM) in mares at the third stage of labor and placental expulsion time in order to investigate a cause of retained placenta of which the incidence increase in a heavy draft mare. Blood was sampled every 5 min from foaling to expulsion of the placenta in 18 heavy draft mares to evaluate circulating OT and PGFM. The relationships between OT and PGFM concentration and recorded placental expulsion times were investigated. The results were as follows (1) The highest level of OT concentration was observed close to foaling in 15 mares. (2) The OT concentrations close to foaling were variable with a large difference from the lowest concentration, 22.1...
Palavras-chave: Heavy draft mare; Oxytocin; 15-keto-dihydro prostaglandin F2α (PGFM); Placental expulsion.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3089
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Relationship between the First Ovulation within Three Weeks Postpartum and Subsequent Ovarian Cycles and Fertility in High Producing Dairy Cows OAK
KAWASHIMA, Chiho; KANEKO, Etsushi; AMAYA MONTOYA, Carlos; MATSUI, Motozumi; YAMAGISHI, Norio; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; KIDA, Katsuya; MIYAKE, Yoichi; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 石井, 三都夫; 木田, 克弥; 三宅, 陽一; 宮本, 明夫.
The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous...
Palavras-chave: Dairy cow; First ovulation; Postpartum; Resumption of ovarian cycle.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/916
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Relationship between the Placental Retention Time and the Reproductive Performance at the Foal Heat in Thoroughbred and a Comparison with Heavy Draft OAK
ISHII, Mitsuo; AOKI, Takahiro; YAMAKAWA, Kazuhiro; MAGATA, Fumie; GOJO, Chikara; ITO, Katsumi; KAYANO, Mitsunori; NAMBO, Yasuo; 石井, 三都夫.
The aim of this study was to clarify the relationship between the placental retention time (PRT) and the reproductive performance following mating at the foal heat in Thoroughbreds. For this purpose, we interviewed 292 farmers over a period of 3 years with questionnaires evaluating foaling, expulsion of placenta and reproductive performance at the foal heat in 1,432 mares. The obtained data were later compared with a previous study of heavy draft mares. The average of the PRT of the 1,432 Thoroughbred mares was 58 ± 88 min (mean ± SD). The mean PRT of Thoroughbreds was significantly shorter than that of the148 min of heavy draft mares. The incidences of retained placenta (RP) occurring in the Thoroughbred mares were 5.2 and 4.0%, for over 3 and 4 hr after...
Palavras-chave: Foaling; Heavy draft; Placental retention time; Reproductive performance; Thoroughbred.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3844
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Three Cases of Atypical Bovine Leukosis in Holstein Cows OAK
田川, 道人; 下田, 崇; 富樫, 義彦; 渡辺, 由紀; 古林, 与志安; 古岡, 秀文; 石井, 三都夫; 猪熊, 壽; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; ISHII, Mitsuo; INOKUMA, Hisashi.
体表リンパ節の腫脹, 末梢血中における異型リンパ球出現を伴うリンパ球数の著増などの典型的な牛白血病症状を示していなかったことから, それぞれアミロイドーシス, 胸膜炎, 心疾患が疑われた3頭のホルスタイン種乳雌牛を病理解剖したところ, いずれも牛白血病と診断された. 血液検査および身体検査において著変が観察されない場合でも, 難診断時には鑑別診断リストに牛白血病を加えるべきであると考えられた.
Palavras-chave: 非典型的牛白血病; 鑑別診断.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4005
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Use of Contrast-Enhanced CT in the Diagnosis of Abscesses in Cattle OAK
LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝.
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination.
Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891
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アミロイドネフローゼを疑った片側性化膿性尿細管間質性腎炎の乳牛の1症例 OAK
村田, 征周; 秋場, 由美; 三宅, 拓夫; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 石井, 三都夫; 猪熊, 壽.
分娩後3か月経過した2歳2か月齢のホルスタイン種乳牛に下顎浮腫および水様性下痢を認めた。腎臓の腫大はなく、また血清蛋白電気泳動像はアミロイドパターンを示さなかったが、蛋白尿と著しい低蛋白血症を認めたためアミロイドーシスを疑った。病理学的及び病原学的検査の結果、血行性の細菌感染による片側性慢性化膿性尿細管間質性腎炎と診断された。アミロイドーシスを疑う症例では、尿の性状検査や細菌検査を繰返し実施することにより、早期に原疾患の鑑別診断を行う必要があると思われた。
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2781
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オーストラリア・クイーンズランド大学における産業動物臨床教育 : 教育GP海外視察報告 OAK
山田, 一孝; 石井, 三都夫; 佐々木, 直樹; 古林, 与志安.
http://nichiju.lin.gr.jp/mag/06209/index.html
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2808
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ハタケシメジ抽出物が哺乳子牛の成長および血液学的検査値に与える影響 OAK
稲沢, 直生実; 山川, 和宏; 石井, 三都夫; 李, 奇子; 室谷, 直義; 池水, 智博; 山田, 一孝; ISHII, Mitsuo; YAMADA, Kazutaka.
ハタケシメジ抽出物がホルスタイン種哺乳子牛の成長および血液学的検査値に与える影響について検討した.試験群は,ハタケシメジ抽出物投与群(雌,n=5)と対照群(雌,n=5)とし,投与群には朝晩の哺乳時にハタケシメジ抽出物(160mg)3カプセルを投与した.試験期間は,出生から8週間とした.試験期間中,投与群と対照群との間で増体に差が認められず,投与群の血液学的および血清生化学検査値は対照群と同様の変化を示した.このことから,ハタケシメジ抽出物は,哺乳子牛の健康状態,増体および血液学的値に悪影響を及ぼさないと考えられた.
Palavras-chave: 子牛; ハタケシメジ抽出物; 血液学的検査; 血清生化学検査.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3890
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ペルシュロン種繁殖雌馬にみられた遠隔転移を伴う腎細胞癌の1症例 OAK
久保田, 直樹; 村上, 智亮; 安田, 峰; 松井, 基純; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽.
19歳齢のペルシュロン種繁殖雌馬に血尿が認められた。血尿は止血剤および抗生剤投与によっては改善され なかった。患畜は次第に元気食欲の低下、削痩、貧血を呈し、高度蛋白尿および胸前部冷性浮腫が発現した。 直腸検査により人頭大に腫大した左腎を触知し、腎臓腫瘍を疑った。病理解剖の結果、左腎は27X18Xl0cmに 腫大し、血液に富む巨大な腫瘤形成(13X8X8cm)を認めた。左腎門リンパ節は腫大し、また右腎臓、左右 副腎、肝臓および肺では大小の白色腫瘤を認めた。病理組織学的検査によりこれらの腫瘍はいずれも浸潤性に 増殖する上皮性腫瘍組織であったことから、本症例は腎細胞癌が他の複数の臓器に転移したものと考察した。
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2935
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