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Registros recuperados: 11
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A new RNA picorna-like virus in the cotton pink bollworm Pectinophora gossypiella (Lep. : Gelechiidae) in Egypt IRD
Monsarrat, Annie; Abol-Ela, S.; Abdel-Hamid, I.; Fédière, Gilles; Kuhl, G.; El Husseini, M.; Giannotti, J..
Un nouveau virus infectant le ver rose du cotonnier, #Pectinophora gossypiella$, a été isolé et purifié à partir de larves mortes récoltées en champ de coton. Les virions purifiés de 27 plus ou moins 1 nm de diamètre, ont une forme icosaédrique et contiennent un génome à ARN. Trois protéines capsidaires de 31,7, 32,6 et 47,4 Kd ont été trouvées dans les virions. Le virus purifié n'est pas hautement infectieux pour les larves, cependant la durée du stade chrysalide est supérieure chez les lots infectés artificiellement. Dans les tissus atteints, les virions sont dispersés dans le cytoplasme des cellules de l'intestin ou bien groupés en amas paracristallins de même type que chez d'autres insectes infectés avec des picornavirus. Les adultes malades...
Tipo: Text Palavras-chave: INSECTE NUISIBLE; COTON; VIRUS; PURIFICATION; ARN; PROTEINE; ULTRASTRUCTURE; POUVOIR PATHOGENE; PARASITOIDE.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010006113
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A small RNA virus isolated from the maize stem borer Sesamia cretica Led. (Lepidoptera : Noctuidae) in Egypt IRD
Fédière, Gilles; Taha, A.A.; Giannotti, J.; Zeddam, Jean-Louis; Monsarrat, Annie; Abol-Ela, S..
Tipo: Text Palavras-chave: INSECTE NUISIBLE; PHYTOPHAGE; LARVE; LUTTE BIOLOGIQUE; VIRUS ENTOMOPATHOGENE; ARN; PROPRIETE BIOLOGIQUE; MICROSCOPIE ELECTRONIQUE; IMMUNODIFFUSION.
Ano: 1991 URL: http://www.documentation.ird.fr/hor/fdi:37669
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Characterization and serology of the leafhopper-borne maize yellow stripe virus in Egypt IRD
Thouvenel, Jean-Claude; Abol-Ela, S.; Sewify, G.H.; El-Hariry, M.; Hussein, A.; Ammar, E.D..
The nucleoprotein and non-capsid protein of maize yellow stripe virus (MYSV) were purified from naturally or experimentally infected maize or sorghum plants. In SDS-PAGE, the apparent molecular weight of the nucleoprotein was 35,6 KD, and that of the non-capsid protein was 14,7 KD. Similar to tenuiviruses, the nucleoprotein of MYSV was associated with fine filaments and the non-capsid protein formed typical needle-shaped crystals. Following a 2-day acquisition feeding period on MYSV-infected plants, vector leafhoppers (#Cicadulina chinai$) remained highly infective for 21 days. Antisera to the nucleoprotein and non-capsid protein of MYSV were produced and used for detection of this virus in several host plants and vector leafhoppers in Egypt. Dot-blot and...
Tipo: Text Palavras-chave: PATHOLOGIE VEGETALE; VIRUS; SEROLOGIE; PROTEINE; MAIZE YELLOW STRIPE VIRUS.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010011007
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Control of the pink borer, Sesamia cretica Led. (Lepidoptera : Noctuidae) in maize field using a granulosis virus in Egypt IRD
Moyal, Pascal; Fédière, Gilles; Semeada, A.M.; El-Sheikh, M.A.K.; El-Sherif, S.; Abol-Ela, S..
The pink borer, #Sesamia cretica$ Lederer (#Lepidoptera$ : #Noctuidae$), is a main pest of maize in Egypt. Attacks by #S. cretica$ are usually high on early plantations of April, when the first generation of adults emerge after a period of larval hibernation. The present study aimed to evaluate the feasibility of the granulosis virus of #S. cretica$, recently isolated by Fediere et al. (1993) as a microbiological agent. A field experiment was conducted in 1997 at Sakha Agricultural Research Station, Kafr El-Sheikh governorate, North of the Nile delta, where heavy natural infestation by #S. cretica$ is of frequent occurrence on early plantations. The experimental design was randomized blocks with seven replications and five treatments. Treatments were...
Tipo: Text Palavras-chave: INSECTE NUISIBLE; LUTTE BIOLOGIQUE; VIRUS; PURIFICATION; INFESTATION; ETUDE EXPERIMENTALE.
Ano: 1997 URL: http://www.documentation.ird.fr/hor/fdi:010016041
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Establishment of a cell line derived from embryos of the potato tuber moth Phthorimaea operculella (Zeller) IRD
Léry, Xavier; Zeddam, Jean-Louis; Giannotti, J.; Croizier, L.; Fédière, Gilles; Abol-Ela, S..
Une lignée cellulaire a été obtenue à partir d'embryocultures de #Phthorimaea operculella$ (Zeller), le principal ravageur de la pomme de terre dans les régions tropicales et sub-tropicales. La lignée cellulaire, nommée ORS-Pop-93, cultivée en milieu Grace modifié, a une population hétérogène de cellules arrondies et allongées très adhérentes. Le temps de doublement des cellules est de 40 h. La lignée a été subcultivée plus de 40 fois. Le profil polypeptidique est différent de celui d'autres lignées de lépidoptères. La lignée est permissive à la polyhédrose nucléaire de #Autographa californica$. (Résumé d'auteur)
Tipo: Text Palavras-chave: ENTOMOLOGIE APPLIQUEE; INSECTE NUISIBLE; POMME DE TERRE; EMBRYON ZYGOTIQUE; CULTURE IN VITRO; LIGNEE CELLULAIRE.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010004859
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Establishment of two cell lines from embryonic cells of Pectinophora gossypiella (Lepidoptera : Gelichiidae) IRD
Léry, Xavier; El-Tarras, A.; Monsarrat, Annie; Abol-Ela, S.; Giannotti, J..
Two cell lines from the most important insect pest of cotton, #Pectinophora gossypiella$ S., were obtained from an embryonic tissue culture. The cells were cultured in GRACE's modified medium. The first cell line, designated ORS-PgS (PgS), was obtained by selecting the non-adherent cells during subculturing and consisted of a homogeneous population of rounded cells. The second cell line, designated ORS-PgA (PgA), obtained without any artificial selection, was a heterogeneous population of spherical and spindle-shaped cells with a great capacity to adhere. The two cell lines had a doubling time of 40 and 48 h respectively and were sub-cultured for more than 50 passages. Their polypeptidic profiles appeared identical but were different from those of other...
Tipo: Text Palavras-chave: INSECTE NUISIBLE; COTON; MILIEU DE CULTURE; VIRUS ENTOMOPATHOGENE; CULTURE CELLULAIRE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010012227
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Host range of the Autographa californica multicapsid nuclear polyhedrosis virus (ACMNPV) in relation to granulosis viruses susceptible lepidopteran species in Egypt IRD
Taha, A.; Giannotti, J.; Léry, Xavier; Ravalec, M.; Abol-Ela, S..
Le spectre d'hôtes de la polyhédrose nucléaire de #Autographa californica$ a été étudié en Egypte sur 3 ravageurs susceptibles à des granuloses. Parmi les insectes testés #Spodoptera littoralis$ ravageur du cotonnier, #Sesamia cretica$ foreur du maïs, et #Phthorimaea operculella$ la teigne de la pomme de terre, seuls #S. cretica$ et #P. operculella$ multiplient NPVAc. L'étude ultrastructurale et la détection du virus par sonde nucléique révèlent que #S. cretica$ est très susceptible alors que 5 % seulement d'infection ont été obtenus avec #P. operculella$. (Résumé d'auteur)
Tipo: Text Palavras-chave: ENTOMOLOGIE APPLIQUEE; INSECTE NUISIBLE; COTON; MAIS; POMME DE TERRE; VIRUS; INFECTION; ETUDE EXPERIMENTALE; LUTTE BIOLOGIQUE.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010005795
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Isolation of a new densonucleosis virus from Mythimna loreiyi Dup. (Lep. Noctuidae) in Egypt IRD
Fédière, Gilles; El-Sheikh, M.A.K.; Abol-Ela, S.; Salah, M.; Massri, M.; Veyrunes, J.C..
Tipo: Text Palavras-chave: INSECTE NUISIBLE; VIRUS ENTOMOPATHOGENE; LUTTE BIOLOGIQUE; PURIFICATION; ADN.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:43185
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Multiplication of the patato tuber moth granulosis virus in Phthorimaea operculella cell cultures IRD
Léry, Xavier; Abol-Ela, S.; Giannotti, J..
Tipo: Text Palavras-chave: ENTOMOLOGIE APPLIQUEE; INSECTE NUISIBLE; POMME DE TERRE; LUTTE BIOLOGIQUE; VIRUS; VIRULENCE; CULTURE CELLULAIRE.
Ano: 1995 URL: http://www.documentation.ird.fr/hor/fdi:010005793
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Natural DNA-recombinations of Spodoptera littoralis NPV among field populations in Egypt IRD
Abol-Ela, S.; Khamis, O.; Giannotti, J.; Fédière, Gilles; Léry, Xavier; Belal, M..
Six natural isolates belonging to #Spodotera littoralis$ NPV were identified. Using the restriction endonucleases Eco RI and Bam HI, the DNA profiles of each isolate from Giza, Menya, Kaliubeia-Kaha, Kafr El-Sheikh, Gharbeia and Kaliubeia-Tokh were differentiated from that of the reference Egypt-84. DNA electrophoretic profiles on different isolates collected 5 years later were compared to the difference type. The DNA sequence was not changed trough three serial passages of viral isolates in vivo, while several viral clones were obtained after the 4th passage. (Résumé d'auteur)
Tipo: Text Palavras-chave: INSECTE NUISIBLE; VIRUS; VARIABILITE GENETIQUE; ADN; METHODE D'ANALYSE.
Ano: 1996 URL: http://www.documentation.ird.fr/hor/fdi:010012231
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Use of non-radioactive nucleic probes for epidemiological survey of potato tuber moth granulosis virus IRD
Zeddam, J.L.; El Bolbol, H.; El-Guindy, N.; Lagnaoui, A.; Al-Abssi, A.S.A.; Fédière, Gilles; Léry, Xavier; Monsarrat, Annie; Abol-Ela, S.; Giannotti, Joseph.
Tipo: Text Palavras-chave: POMME DE TERRE; INSECTE NUISIBLE; CHENILLE; LUTTE PHYTOSANITAIRE; LUTTE BIOLOGIQUE; VIRUS ENTOMOPATHOGENE; EPIDEMIOLOGIE; BIOLOGIE MOLECULAIRE; HYBRIDATION; METHODE D'ANALYSE; SONDE NUCLEIQUE; PTM.POTATO TUBER MOTH.
Ano: 1994 URL: http://www.documentation.ird.fr/hor/fdi:010025959
Registros recuperados: 11
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