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Chitosan derivatives induce local and distal expression of defence-related genes in wheat (Triticum aestivum L.) seedlings Agrociencia
Díaz-Martínez,Juan M.; Aispuro-Hernández,Emmanuel; Vargas-Arispuro,Irasema; Falcón-Rodríguez,Alejandro B.; Martínez-Téllez,Miguel Á..
Abstract Chitosan and its derivatives are elicitors of defence responses in plants. The physical and chemical characteristics, like the molecular weight and acetylation degree determine the type of effect and specificity. Although chitosan-induced biochemical and enzymatic reactions in wheat (Triticum aestivum) are reported, the transcriptional activation of defensive markers by chitosan derivatives remains unaddressed. The hypothesis of our study was that the different structural features of chitosan derivatives would influence the transcriptional patterns related to protective responses in wheat. The aim of our study was to compare the local and systemic inductive properties of chitosan and chitooligosaccharides (COS) in wheat seedlings through the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitinase; Chitooligosaccharide; Glucanase; Phenylalanine ammonia-lyase; Systemic resistance.
Ano: 2018 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000400497
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Total RNA quality of lyophilized and cryopreserved dormant grapevine buds Electron. J. Biotechnol.
García-Baldenegro,Claudia Vanessa; Vargas-Arispuro,Irasema; Islas-Osuna,María; Rivera-Domínguez,Marisela; Aispuro-Hernández,Emmanuel; Martínez-Téllez,Miguel Ángel.
Background Plant tissues must be preserved in their collection state, especially for genome-wide expression profile studies. Lyophilization is a feasible, affordable tool when fresh tissues cannot be shipped at ultralow temperatures from their origin to the place of analysis. In this study, the total RNA quality of dormant grapevine buds (Vitis vinifera L. cv. ‘Flame Seedless’) of freeze-dried samples stored at room temperature conditions was evaluated and compared to that of cryopreserved (-80°C) grapevine buds. Results Good yield and quality of RNA were obtained from freeze-dried dormant buds stored at room temperature for 0, 3 and 6 weeks after they were lyophilized. Further experiments confirmed that the extracted total RNA could be used for actin and...
Tipo: Journal article Palavras-chave: Freeze-dried; RNA integrity number; RT-qPCR; Vitis vinifera L; Woody tissue.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200012
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