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Characterization of 41 microsatellite loci developed from the genome sequence of the poplar rust fungus, Melampsora larici-populina Inra
Xhaard, C.; Andrieux, A.; Halkett, F.; Frey, P..
Forty-one microsatellite markers were developed for the poplar rust fungus Melampsora larici-populina. The genome sequence of M. larici-populina strain 98AG31 was screened in silico for microsatellite loci using Magellan software. Thirty-nine loci, evaluated on a sexual population of 35 M. larici-populina isolates sampled on larch trees in the French Alps, were identified to be highly polymorphic. These markers revealed two to 34 alleles, with an average of 7.23 alleles per locus. The estimated heterozygosity ranged from 0.029 to 0.958 across the 41 microsatellite loci. These markers will be highly useful for population genetics studies of M. larici-populina.
Tipo: Journal Article Palavras-chave: BASIDIOMYCETE; MELAMPSORA LARICI-POPULINA; POPLAR RUST FUNGUS; GENOME SEQUENCE; MICROSATELLITES; M13-TAILED PRIMER METHOD.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010e8fd96fd&uri=/notices/prodinra1/2010/10/
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Distribution and expression of elicitin genes in the interspecific hybrid oomycete Phytophthora alni. Inra
Ioos, R.; Panabières, F.; Industri, B.; Andrieux, A.; Frey, P..
Phytophthora alni subsp. alni (Paa), P. alni subsp. multiformis (Pam) and P. alni subsp.uniformis (Pau) are responsible for alder disease in Europe. Class I and II elicitin genes patterns of Paa, Pam, Pau and the phylogenetically close species P. cambivora and P.fragariae were studied through mRNA sequencing and 3’ untranslated region (3’UTR)-specific PCRs and sequencing. The occurrence of multiple 3’UTR sequences in association to identical elicitin encoding sequences in Paa indicated duplication/recombination events. The mRNA pattern displayed by Paa demonstrated that elicitin genes from all the parental genomes are actually expressed in this allopolyploid taxon. The complementary elicitin patterns resolved confirmed the possible involvement of Pam and...
Tipo: Journal Article Palavras-chave: HYBRIDATION INTERSPECIFIQUE PHYTOPHTHORA ALNI; PHYTOPHTHORA ALNI SSP ALNI; PHYTOPHTHORA ALNI SSP MULTIFORMIS; PHYTOPHTHORA ALNI SSP UNIFORMIS.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20079b0f2c9d&uri=/notices/prodinra1/2007/10/
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Genetic structure of the poplar rust fungus Melampsora larici-populina : Evidence for isolation by distance in Europe and recent founder effects overseas Inra
Barres, B.; Halkett, F.; Dutech, C.C.; Andrieux, A.; Pinon, J.; Frey, P..
Dispersal has a great impact on the genetic structure of populations, but remains difficult to estimate by direct measures. In particular, gradual and stochastic dispersal are often difficult to assess and to distinguish, although they have different evolutionary consequences. Plant pathogens, especially rust fungi, are suspected to display both dispersal modes, though on different spatial scales. In this study, we inferred dispersal capacities of the poplar rust fungus Melampsora larici-populina by examining the genetic diversity and structure of 13 populations from eight European and two overseas countries in the Northern hemisphere. M. larici-populina was sampled from both cultivated hybrid poplars and on the wild host, Populus nigra. The populations...
Tipo: Journal Article Palavras-chave: ROUILLE; DISPERSION; PLANTE PATHOGÈNE; DIVERSITÉ GENETIQUE MELAMPSORA LARICI-POPULINA; POPULUS NIGRA.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008b7cff2a9&uri=/notices/prodinra1/2009/02/
Registros recuperados: 3
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