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A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes ArchiMer
Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf
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Etude des mouvements calciques dans les hémocytes de l'huître creuse Crassostrea gigas : 1ères approches expérimentales ArchiMer
Aton, Emilie.
Les cultures de mollusques bivalves sont continuellement exposées à des variations de facteurs d'origine naturelle (variation de la salinité et de température) ou anthropique (métaux lourds, HAP, phytosanitaires) susceptibles d'altérer les systèmes de défense des mollusques bivalves. Ces altérations expliqueraient, dans certains cas, leur sensibilité vis à vis de certains pathoègnes marins et pourraient, en partie, être à l'origine des épisodes de mortalité massive observés périodiquement chez des populations larvaires d'huîtres et de moules. C'est pourquoi de nombreuses études concernant l'immunotoxicité des polluants sont actuellement en cours. Le calcium est un second messager ubiquitaire connu pour assurer la transduction de très nombreux signaux...
Tipo: Text Palavras-chave: Huître creuse; Crassostrea gigas; Hémocytes; Calcium; Transfert calcique; Impact polluant.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00032/14336/11615.pdf
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Etude des mouvements calciques dans les hémocytes de l'huître creuse Crassostrea gigas lors de leur immunostimulation : 1ères approches expérimentales ArchiMer
Aton, Emilie; Cognard, C.; Renault, Tristan; Thomas-guyon, Hélène; Imbert, N..
Bivalve mollusc farms are continuously exposed to variations of factors either natural (variation of salinity and temperature) or from man (heavy metals, HAP, phytosanitary) likely to alter the defence systems of bivalve molluscs. These alterations would explain, in certain cases, their sensitivity vis-à-vis certain marine pathogens and could in part be at the source of the episodes of massive mortalities seen periodically in oyster and mussel larval populations. That is why many studies concerning the contaminants' immunotoxicity are currently in progress.
Tipo: Text Palavras-chave: Polluant; Calicum; Hemocytes; Crassostrea gigas.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2855.pdf
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