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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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