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Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars Electron. J. Biotechnol.
Rashid,Umer; Ali,Shaukat; Ali,Ghulam Muhammad; Ayub,Najma; Masood,M. Shahid.
Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested. Best hormone combinations were further subjected to Kinetin and 6-γ-γ-dimethylallylaminopurine (2iP). For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25%, 77.75% and 95.20% of embryogenic calli, respectively. Maximum callus induction (97.18%) was observed in Tatara when 2 mg/L of 2,4-D was used. As regard...
Tipo: Journal article Palavras-chave: 2; 4-D 2iP BAP callus induction IAA kinetin regeneration wheat.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300004
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