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Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) Genet. Mol. Biol.
Costa,Laiana Cristina da; Azeredo-Oliveira,Maria Tercília Vilela de; Tartarotti,Ester.
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triatoma klugi; Triatomine; Cytogenetics; Nucleolus; Karyotype; Meiosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008
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Spermatogenesis of Zaprionus indianus and Zaprionus sepsoides (Diptera, Drosophilidae): cytochemical, structural and ultrastructural characterization Genet. Mol. Biol.
Rego,Letícia do Nascimento Andrade de Almeida; Silistino-Souza,Rosana; Azeredo-Oliveira,Maria Tercília Vilela de; Madi-Ravazzi,Lilian.
Zaprionus indianus is a drosophilid native to the Afrotropical region that has colonized South America and exhibits a wide geographical distribution. In contrast, Z. sepsoides is restricted to certain African regions. The two species differ in the size of their testes, which are larger in Z. indianus than in Z. sepsoides. To better understand the biology and the degree of differentiation of these species, the current study evaluated spermatogenesis in males of different ages by conventional staining techniques and ultrastructural analysis. Spermatogenesis and the ultrastructure of spermatozoa were similar in the two species, and the diploid number was confirmed to be 2n = 12. A greater number of spermatozoa were observed in young Z. indianus (1-3 days old)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zaprionus indianus; Zaprionus sepsoides; Spermatogenesis; Cytochemistry; Ultrastructure; Meiosis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100008
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