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Registros recuperados: 5
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Analysis of myosin heavy chains at the protein level in horse skeletal muscle Inra
Rivero, J.L.L.; Serrano, A.L.; Barrey, E.; Valette, J.P.; Jouglin, M..
Combined methodologies of enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulphate polyacrilamide gel electrophoresis (SDS-PAGE), immunoblotting, traditional myofibrillar ATPase (mATPase) histochemistry and immunocytochemistry of whole biopsied samples were used to study myosin heavy chain (MHC) isoforms in the equine gluteus medius muscle. The ELISA technique allowed the quantification of the three MHC isoforms known to be present in different horse muscles: slow (MHC-I) and two fast (termed MHC-IIA and MCH-IIX). The SDS-PAGE method resolved MHCs in three bands: MHC-I, MHC-IIX and MHC-IIA from the fastest to the slowest migrating band and a quantification by densitometry for each MHC isoform was also possible. The identity of these three MHCs...
Tipo: Journal Article Palavras-chave: MYOSINE; PROTEINE MUSCULAIRE; EQUIDAE; MUSCLE SQUELETTIQUE; ELISA; TECHNIQUE ANALYTIQUE.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0100029041089121&uri=/notices/prodinra1/2010/12/
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Microarray analysis after strenuous exercise in peripheral blood mononuclear cells of endurance horses Inra
Capomaccio, S.; Cappelli, K.; Barrey, E.; Felicetti, M.; Silvestrelli, M.; Verini-Supplizi, A..
It is known that moderate physical activity may have beneficial effects on health, whereasstrenuous effort induces a state resembling inflammation. The molecular mechanismsunderlying the cellular response to exercise remain unclear, although it is clear that theimmune system plays a key role. It has been hypothesized that the physio-pathologicalcondition that develops in athletes subjected to heavy training is caused by derangement ofcellular immune regulation. The purpose of the present study was to obtain information onendurance horse gene transcription under strenuous conditions and to identify candidategenes causing immune system derangement. We performed a wide gene expression scan,using microarray technology, on peripheral blood mononuclear cells of...
Tipo: Journal Article Palavras-chave:  gene expression inflammation peripheral blood mononuclear cells (PBMCs); QRT-PCR stress.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20113718b22d&uri=/notices/prodinra1/2011/04/
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Muscular microRNA expressions in healthy and myopathic horses suffering from polysaccharide storage myopathy or recurrent exertional rhabdomyolysis Inra
Barrey, E.; Bonnamy, B; Barrey, E.J.; Mata, X.; Chaffaux, S.; Guérin, G..
Reasons for performing study: MicroRNAs (miRNA) are small endogenous noncoding interfering RNA molecules (18–25 nucleotides) regarded as major regulators in eukaryotic gene expression. They play a role in developmental timing, cellular differentiation, signalling and apoptosis pathways. Because of the central function of miRNAs in the proliferation and differentiation of the myoblasts demonstrated in mouse and man, it is assumed that they could be present in equine muscles and their expression profile may be related to the muscle status.Objective: To identify miRNA candidates in the muscles of control and affected horses suffering from polysaccharide storage myopathy (PSSM) and recurrent exertional rhabdomyolysis (RER).Methods: Muscle biopsies were...
Tipo: Journal Article Palavras-chave: Horse; Transcriptomic; MiRNA profile; Myopathy; RER; PSSM.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010aa2098f0&uri=/notices/prodinra1/2011/02/
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Proprietes contractiles des fibres musculaires et performance physique chez le cheval Inra
Barrey, E..
Cet article présente les connaissances acquises sur la physiologie des fibres musculaires en relation avec l’exercice physique chez le cheval-athlète. La vitesse et la force de contraction développée par un muscle dépendent à la fois de la quantité de fibres actives et de leurs propriétés contractiles. Le muscle étant le moteur de l’athlète, de nombreuses études de physiologie de l’exercice ont porté sur la typologie musculaire afin d’en connaître le déterminisme et les potentialités adaptatives en réponse à un travail d’entraînement. Les méthodes d’investigation, les caractéristiques fonctionnelles, les facteurs de variation et les relations avec la performance physique sont présentés et discutés dans le cas du cheval pour envisager une application à la...
Tipo: Journal Article Palavras-chave: ATHLETE; TRAVAIL D'ENTRAINEMENT; PERFORMANCE PHYSIQUE; TYPOLOGIE MUSCULAIRE; FIBRE MUSCULAIRE; METHODE D'ANALYSE; TYPAGE.
Ano: 1994 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB9500032332044447&uri=/notices/prodinra1/2008/02/
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Review: Genetics and genomics in equine exercise physiology: an overview of the new applications of molecular biology as positive and negative markers of performance and health Inra
Barrey, E..
Equine breeding selection has been developed by applying quantitative genetic methods for calculating the heritability of the complex traits such as performance in racing or sport competitions. With the great development of biotechnologies, equine molecular genetics has come of age. The recent sequencing of the equine genome by an international consortium was a major advance that will impact equine genomics in the near future. With the rapid progress in equine genetics, new applications in early performance evaluation and the detection of disease markers become available. Many new biomolecular tools will change management of horse selection, disease diagnosis and treatment. The purpose of this review is to present new developments in equine genetics and...
Tipo: Journal Article Palavras-chave: Horse; Gene; Transcript; RNA; DNA; Microarray; SNP; Exercise; Muscle.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20101f0f686a&uri=/notices/prodinra1/2011/02/
Registros recuperados: 5
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