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Registros recuperados: 18
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Accumulation and mobilization of lipids in relation to reproduction of yellowfin tuna (Thunnus albacares) in the Western Indian Ocean ArchiMer
Zudaire, Iker; Murua, Hilario; Grande, Maitane; Pernet, Fabrice; Bodin, Nathalie.
Total lipid content and lipid class composition were analyzed in gonads, liver and white muscle of yellowfin tuna (Thunnus albacares) throughout ovary development to understand its reproductive allocation strategy and to assess the relation between female condition and reproduction. A total of 112 females were collected onboard purse-seiner in the Western Indian Ocean from January to March 2009, from June to July 2009, and from April to May 2010. Gonads were characterized by highly variable total lipid contents ranging from 5 to 27 μg mg−1 of wet weight (ww) with a predominance of neutral lipids, mainly triacylglycerols (TAG) and sterol- and wax-esters. The different lipid classes in gonads described an accumulative pattern through the maturity process...
Tipo: Text Palavras-chave: Tropical tuna; Lipid class composition; Reproductive allocation strategy; Income-capital breeder; Fecundity; FADs.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28266/28075.pdf
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Chemical contaminants (trace metals, persistent organic pollutants) in albacore tuna from western Indian and south-eastern Atlantic Oceans: Trophic influence and potential as tracers of populations ArchiMer
Chouvelon, Tiphaine; Brach-papa, Christophe; Auger, Dominique; Bodin, Nathalie; Bruzac, Sandrine; Crochet, Sylvette; Degroote, Maxime; Hollanda, Stephanie J.; Hubert, Clarisse; Knoery, Joel; Munschy, Catherine; Puech, Alexis; Rozuel, Emmanuelle; Thomas, Bastien; West, Wendy; Bourjea, Jerome; Nikolic, Natacha.
Albacore tuna (Thunnus alalunga) is a highly commercial fish species harvested in the world's Oceans. Identifying the potential links between populations is one of the key tools that can improve the current management across fisheries areas. In addition to characterising populations' contamination state, chemical compounds can help refine foraging areas, individual flows and populations' structure, especially when combined with other intrinsic biogeochemical (trophic) markers such as carbon and nitrogen stable isotopes. This study investigated the bioaccumulation of seven selected trace metals – chromium, nickel, copper (Cu), zinc (Zn), cadmium (Cd), mercury (Hg) and lead – in the muscle of 443 albacore tunas, collected over two seasons and/or years in the...
Tipo: Text Palavras-chave: Top predator; Bioaccumulation; Inorganic elements; Organic contaminants; Stable isotopes; Intrinsic markers.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00384/49532/51152.pdf
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Comparaison des variations saisonnières de la maturation des gonades et de biomarqueurs chez la moule (Mytilus galloprovincialis) ArchiMer
Bodin, Nathalie.
Au cours de mon stage de maîtrise, ma contribution à cette étude a pour objectifs: (1) évaluer le stade de la maturation des gonades et déterminer le sexe des moules échantillonnées, à l'aide d'une technique d'analyse histologique. Ce travail a pour but de valider le développement gonadique comme indicateur physiologique du niveau de contamination du milieu. (2) comparer les variations saisonnières de la maturation des gonades et les sexes ratio avec des indices physiologiques tel que l'indice de condition et l'indice de croissance, un biomarqueur (les adduits à l'ADN), les paramètres physico-chimiques du milieu (température, salinité, turbidité), et la contamination chimique des tissus des moules par les PCB, les HAP, et les métaux lourds. Ce travail...
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Ano: 2001 URL: http://archimer.ifremer.fr/doc/00425/53684/54529.pdf
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Contamination des crustacés décapodes par les composes organohalogénés : étude détaille de la bioaccumulation des PCB chez l'araignée de mer Maja brachydactyla ArchiMer
Bodin, Nathalie.
Marine decapod crustaceans are macrobenthic opportunist species, of great commercial interest, and can accumulate pollutants. Worrying about consumers' safety, polychlorobiphenyls (PCBs), polybromodiphenyl Ethers (PBDEs) and dioxins (PCDD/Fs) were analyzed in four crustacean species from the coastal waters of Brittany and Normandy. As regards to regulations, the consumption of crustacean muscle doesn't raise any health problems. The second objective of this study was to evaluate the biological processes influencing PCB bioaccumulation in the spider crab Maja brachydactyla. From a quantitative point of view, organotropism, diet and age were the major factors acting on contamination. From a qualitative point of view, PCB fingerprints depended on sex, and to...
Tipo: Text Palavras-chave: Food safety; Biotransformation; Biomagnification; Food webs; Stable isotopes; Decapod crustaceans; Bioaccumulation; Polychlorinated biphenyls; Organic contaminants; Sécurité sanitaire; Biotransformation; Bioamplification; Réseaux trophiques; Isotopes stables; Crustacés décapodes; Bioaccumulation; Contaminants organiques.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-1149.pdf
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Determining the age of tropical tunas in the Indian Ocean from otolith microstructures ArchiMer
Sardenne, Fany; Dortel, Emmanuelle; Le Croizier, Gael; Million, Julien; Labonne, Maylis; Leroy, Bruno; Bodin, Nathalie; Chassot, Emmanuel.
The Indian Ocean Tuna Tagging Program (IOTTP) provided a unique opportunity to assess the viability of estimating the age of tropical tunas from the micro-structural features of otoliths. Here, we analyzed the length measurements and micro-increment counts collected for 506 sagittal otoliths, of which 343 were chemically marked with oxytetracycline, for bigeye (Thunnus obesus), skipjack (Katsuwonus pelamis), and yellowfin tuna (Thunnus albacares). Our results show that the otoliths of tropical tunas grow more slowly than the rest of the body. Our findings confirm that both yellowfin and juvenile bigeye deposit daily increments in their otoliths, though ages are underestimated for large bigeye (>100 cm) when derived from micro-increment counts. Our...
Tipo: Text Palavras-chave: Daily age; Micro-increment; Mark-recapture; Oxytetracycline; Thunnus.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36511/35082.pdf
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Discovery of Genome-Wide Microsatellite Markers in Scombridae: A Pilot Study on Albacore Tuna ArchiMer
Nikolic, Natacha; Duthoy, Stephanie; Destombes, Antoine; Bodin, Nathalie; West, Wendy; Puech, Alexis; Bourjea, Jerome.
Recent developments in sequencing technologies and bioinformatics analysis provide a greater amount of DNA sequencing reads at a low cost. Microsatellites are the markers of choice for a variety of population genetic studies, and high quality markers can be discovered in non-model organisms, such as tuna, with these recent developments. Here, we use a high-throughput method to isolate microsatellite markers in albacore tuna, Thunnus alalunga, based on coupling multiplex enrichment and next-generation sequencing on 454 GS-FLX Titanium pyrosequencing. The crucial minimum number of polymorphic markers to infer evolutionary and ecological processes for this species has been described for the first time. We provide 1670 microsatellite design primer pairs, and...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00293/40460/39151.pdf
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Effects of extraction method and storage of dry tissue on marine lipids and fatty acids ArchiMer
Sardenne, Fany; Bodin, Nathalie; Metral, Luisa; Crottier, Anais; Le Grand, Fabienne; Bideau, Antoine; Brisset, Blandine; Bourjea, Jerome; Saraux, Claire; Bonhommeau, Sylvain; Kerzerho, Vincent; Bernard, Serge; Rouyer, Tristan.
Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared. Three dry storage modes of tissue (freezer vs gas nitrogen vs dry room) during one and three months were also compared. These comparisons were made on seven marine species with different lipid profiles, including fishes, crustaceans and mollusks. Lipid classes and FA...
Tipo: Text Palavras-chave: Degradation; Freeze-drying; Lyophilization; Lipid class; Marine animal; Protocol.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00467/57898/60189.pdf
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Etude des variations saisonnières de différents biomarqueurs, chez la moule Mytilus galloprovincialis. Relation avec certains paramètres physiques, chimiques et physiologiques ArchiMer
Bodin, Nathalie.
The aim of this study was to evaluate the seasonal variations of six biomarkers on Mytilus galloprovincialis, using comparison with physiological index and chemical contamination levels on two sites of  Mediterranean sea. The seasonal variations of acetylcholinesterase (AChE), DNA adducts, benzo(a)pyrene hydroxylase (BPH), heat-shock proteins (HSP), metallothioneins (MT) and multixeniobiotic proteins (MXR) were estimated over a two-year period, on mussels which were sampled in Carteau (original site) and La Fourcade (transplanted organisms). Carteau, an industrial site, presents higher contamination levels (HAP, PCB, heavy metals) than La Fourcade, an urban area. The PAH profiles underline a strong spatio-temporal variability of levels and sources of...
Tipo: Text Palavras-chave: Biomonitoring; Mytilus galloprovincialis; Biomarqueurs; Variations saisonnières; Biomonitoring; Mytilus galloprovincialis; Biomarkers; Seasonal variations.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00443/55499/57042.pdf
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FADS2: A potential limiting step in HUFA metabolic pathway of European sea bass (Dicentrarchus labrax) ArchiMer
Geay, E; Santigosa I Culi, E; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y; Corcos, L; Bodin, Nathalie; Kaushik, Sadasivam; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
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Ano: 2010
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Organochlorinated contaminants in decapod crustaceans from the coasts of Brittany and Normandy (France) ArchiMer
Bodin, Nathalie; Abarnou, Alain; Le Guellec, Anne-marie; Loizeau, Veronique; Philippon, Xavier.
The contamination and distribution of organ ochlorinated compounds were considered in three crustacean species (edible crab, Cancer pagurus; spider crab, Maja brachydactyla; velvet swimming crab, Necora puber) from five sites along the coasts of Brittany and Normandy (Western and North-Western France). PCBs (16 single congeners), pp'-DDE and HCB were measured in hepatopancreas, gonads and muscle: in all, 175 samples were analysed. The spider crab was the only species found in the five sampling sites, thus enabling comparison between areas. Specimens from Antifer were much more contaminated (Sigma(16)PCBs in hepatopancreas = 2000-4000 ng g(-1) dry weight) than those from other sites (50-1000 ng g(-1) d.w.). Among all the three species, the spider crab...
Tipo: Text Palavras-chave: Human diet; France; Brittany; Normandy; Crustaceans; Polychlorobiphenyls.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2594.pdf
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Organohalogen compounds in yellowfin tuna (Thunnus albacares) from the western Indian ocean ArchiMer
Torres, Joao Paulo Machado; Munschy, Catherine; Heas-moisan, Karine; Potier, Michel; Menard, Frederic; Bodin, Nathalie.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00077/18866/16445.pdf
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PCB, PCDD/F and PBDE levels and profiles in crustaceans from the coastal waters of Brittany and Normandy (France) ArchiMer
Bodin, Nathalie; Abarnou, Alain; Fraisse, D; Defour, S; Loizeau, Veronique; Le Guellec, Anne-marie; Philippon, Xavier.
Polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs) and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) were analysed in the muscle of various edible marine crustaceans (spider crab, edible crab, velvet swimming crab and Norway lobster) from the Brittany and Normandy coasts (France). The highest concentrations were measured in species collected from Antifer (Seine Bay). PCB and PBDE patterns in crustacean muscles were similar and independent of the geographical area with the predominance of the high chlorinated PCBs (CB 15 3, 138, 118 and 180), and of a few PBDE congeners (BDE47, BDE99, WE 100 and BDE28). Oppositely, dioxin contamination differed with site. The major component in crustaceans from the Seine Bay was...
Tipo: Text Palavras-chave: France; Food safety; Metabolisation; Decapod crustaceans; PBDEs; PCDD/Fs; PCBs.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2685.pdf
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Projet GERMON. Structure génétique et migration du thon Germon ArchiMer
Nikolic, Natacha; Puech, Alexis; Chouvelon, Tiphaine; Munschy, Catherine; Bodin, Nathalie; Brach-papa, Christophe; Potier, Michel; West, Wendy; Knoery, Joel; Zudaire, Iker; Dhurmeea, Zahirah; Degroote, Maxime; Cedras, Maria; Evano, Hugues; Bourjea, Jerome.
The GERMON project had several aims i) to understand the populations’ structure between albacore tuna southwest of the Indian Ocean and southeast Atlantic, ii) to determine the origin of juveniles observed in South Africa iii) to improve the understanding of the biology field (Size/Size and Size/Weight; reproduction and feeding area; trophic linkages) and iv) to initiate work on chemical analyzes and adaptation of the albacore species to its environment. These aims have been held and the results allow a better understanding of the management albacore. A general summary of results is presented in Part 9.
Tipo: Text Palavras-chave: Germon; Thunnus alalunga; Génétique; Stock; Structure; Connectivité; Chimie; Reproduction; Alimentation; Habitat; Albacore; Thunnus alalunga; Genetic; Stock; Structure; Connectivity; Chemical; Reproduction; Feeding behavior; Habitat.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00293/40461/39152.pdf
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Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet ArchiMer
Geay, Florian; Culi, E. Santigosa I.; Corporeau, Charlotte; Boudry, Pierre; Dreano, Y.; Corcos, L.; Bodin, Nathalie; Vandeputte, Marc; Zambonino, Jose-luis; Mazurais, David; Cahu, Chantal.
Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol...
Tipo: Text Palavras-chave: Sea bass (Dicentrarchus labrax); FADS2 expression and activity; HUFA biosynthesis.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00008/11929/8635.pdf
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Reproductive Biology of Albacore Tuna (Thunnus alalunga) in the Western Indian Ocean ArchiMer
Dhurmeea, Zahirah; Zudaire, Iker; Chassot, Emmanuel; Cedras, Maria; Nikolic, Natacha; Bourjea, Jerome; West, Wendy; Appadoo, Chandani; Bodin, Nathalie.
The reproductive biology of albacore tuna, Thunnus alalunga, in the western Indian Ocean was examined through analysis of the sex ratio, spawning season, length-at-maturity (L50), spawning frequency and fecundity. From 2013 to 2015, a total of 923 female and 867 male albacore were sampled. A bias in sex ratio was found in favor of females with fork length (LF) < 100 cm. Using histological analyses and gonadosomatic index, spawning was found to occur between 10°S and 30°S, mainly to the east of Madagascar from October to January. Large females contributed more to reproduction through their longer spawning period compared to small individuals. The L50 (mean ± standard error) of female albacore was estimated at 85.3 ± 0.7 cm LF. Albacore spawn on average...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00362/47284/47261.pdf
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Seasonal habitat and length influence on the trophic niche of co-occurring tropical tunas in the eastern Atlantic Ocean ArchiMer
Sardenne, Fany; Diaha, N'Guessan Constance; Amande, Monin Justin; Zudaire, Iker; Couturier, Lydie; Metral, Luisa; Le Grand, Fabienne; Bodin, Nathalie.
In the Gulf of Guinea, bigeye Thunnus obesus (BET) and yellowfin tuna Thunnus albacares (YFT) are important for commercial fisheries and play a prominent ecological role as top predators. Using fatty acid profiles and carbon and nitrogen stable isotopes we examined their niche partitioning in this understudied region. Niche overlap was high (>70%), similar to percentages in other ocean basins. BET occupied a higher trophic position than YFT and fed on deeper prey (high δ15N values and high proportions of mono-unsaturated fatty acids). The trophic position of YFT decreased slightly in the last 15 years (δ15N values decrease ~0.5 ‰) suggesting a change in epipelagic communities, as observed in the eastern Pacific Ocean. Ontogenic changes were limited to...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00436/54796/56254.pdf
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Temporal and inter-specific variations in forage fish feeding conditions in the NW Mediterranean: lipid content and fatty acid compositional changes ArchiMer
Pethybridge, H.; Bodin, Nathalie; Arsenault-pernet, E. -j.; Bourdeix, Jean-herve; Brisset, Blandine; Bigot, Jean-louis; Roos, David; Peter, M..
We describe the total lipid content, lipid class composition and fatty acid profiles of adult forage fishes (anchovy, sardine and sprat) sampled in the NW Mediterranean Sea in 2010 and 2011. Inter-and intra-species differences were mostly related to sampling period with limited effect of gender or total length. As an assemblage, total lipid content and relative levels of triacylglycerols and fatty acids 16:1n7, 20:5n3 and 14:0 in forage fish were highest in summer and autumn, indicating better feeding conditions and a more pronounced diatom-supported food web. In contrast, total lipid content was lowest at the end of winter and spring, and coincided with high levels of 22:6n3, indicating a more herbivorous diet based on dinoflagellates. Resource...
Tipo: Text Palavras-chave: Trophic markers; Environmental variations; Clupeiformes; Nutritional condition; Prey quality; Ecosystem health.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00239/34997/33530.pdf
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Variablility of stable isotope signatures (delta C-13 and delta N-15) in two spider crab populations (Maja brachydactyla) in Western Europe ArchiMer
Bodin, Nathalie; Le Loch, Francois; Hily, Christian; Caisey, Xavier; Latrouite, Daniel; Le Guellec, Anne-marie.
The ontogenic variations of nitrogen and carbon stable isotopic signatures (delta N-15 and delta C-13) were investigated in two spider crab (Maja brachydactyla) populations inhabiting in different biotopes of Western Europe. The Iroise Sea population is localized in Western Brittany and characterized by a seasonal migration occurring on a large bathymetric and habitat gradient while the Seine Bay population, in the Eastern English Channel, remains in a more homogeneous environment during its migration. In the Iroise Sea population, delta C-13 Values increased significantly both with body size and age, revealing a shift towards "benthic-component" prey with spider crab growth. On the contrary, neither body size nor ageing gave rise to a significant trophic...
Tipo: Text Palavras-chave: Migration; Marine decapod crustaceans; Growth; Feeding; Carbon and nitrogen stable isotopes.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2535.pdf
Registros recuperados: 18
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