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Registros recuperados: 41
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Application of POCIS for the monitoring of pesticides, pharmaceuticals and alkylphenols in marine water ArchiMer
Tapie, Nathalie; Munaron, Dominique; Gonzalez, Jean-louis; Budzinski, Helene.
With the new European legislation (the Water Framework Directive, 2000/60/EC), the decision makers responsible for the assessment of the environmental contamination require simple monitoring tools. Passive sampling methods seem to be promising tools for the sampling of polar organic compounds in water, especially in marine waters where important dilutions and low concentrations are usual. They are taking into account the temporal variability and integrating the contamination over period of time. This work presents a study, on a large scale, of the contamination of French Mediterranean coastal waters realized in partnership with the Water Agency Rhône Méditerranée Corse (AERMC), and Coastal Water Quality Offices (CQEL).
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00031/14204/11473.pdf
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Approche multirésidus pour le suivi des pesticides dans différents compartiments (eau, sédiment, huîtres) de l’environnement aquatique du bassin d’Arcachon ArchiMer
Murcia, Fréderic; Le Menach, Karyn; Pardon, P.; Augagneur, S.; Armstrong, N.; Tapie, Nathalie; Auby, Isabelle; Budzinski, Helene.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00259/37069/35581.pdf
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AQUAREF intercomparison passive sampling exercise: monitoring of pesticides in surface water. ArchiMer
Berho, Catherine; Mazzella, Nicolas; Munaron, Dominique; Lepot, Benedicte; Budzinski, Helene; Gonzalez, Catherine; Gravell, Antony; Gunold, Roman; Peinerud, Elsa; Smedes, Foppe; Vrana, Branislav; Miege, Cécile.
The Water Framework Directive (WFD) is probably the most significant legislation in the water field that has been introduced for many years. Several publications pointed out that passive samplers (PSs) can be valuable tools as complementary method in monitoring water quality in the context of the WFD However, there is still a lack of quality assurance and control (QA/QC) procedures that demonstrate the reliability and the comparability of results obtained by passive sampling. Up to date, very few in situ intercomparison exercises on PSs have been performed until now. To our knowledge only three pan-European or international intercomparison studies have been organized in situ since 2007 (ICES Passive sampling survey and intercalibration, AQUAREF, IPSIC and...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27873/26118.pdf
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Assessment of chemical contamination of french coastal lagoons using passive sampling techniques. ArchiMer
Munaron, Dominique; Hubert, Morgane; Tapie, Nathalie; Budzinski, Helene; Guyomarch, Julien; Andral, Bruno; Gonzalez, Jean-louis.
Since 2008, projects supported by french government agencies were dedicated to improve the assessment of chemical contamination of water bodies defined by the Water Framework Directive (WFD, 2000/60/CE)(Munaron et al, 2012). Among these studies, the aim of PEPSLAG was to investigate the utility of passive samplers for sampling contaminants present at trace levels in coastal lagoons environments, and to realize a first assessment of the contamination on the French Mediterranean coastal lagoons. 23 transitional lagoons water-bodies were investigated along the French Mediterranean coast, using three kinds of passive samplers (DGT, POCIS and SBSE). 141 contaminants from various chemical families (trace metals, pesticides, pharmaceuticals, alkylphenols, PAHs,...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00167/27870/26115.pdf
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Can pesticides, copper and seasonal water temperature explain the seagrass Zostera noltei decline in the Arcachon bay? ArchiMer
Gamain, Perrine; Feurtet-mazel, Agnes; Maury-brachet, Regine; Auby, Isabelle; Pierron, Fabien; Belles, Angel; Budzinski, Helene; Daffe, Guillemine; Gonzalez, Patrice.
Dwarf eelgrasses (Zostera noltei) populations have decreased since 2005 in Arcachon Bay (southwest France). Various stressors have been pointed out, however the role of xenobiotics like pesticides or copper (Cu) and of parameters like water temperature warming have not yet been explored. To determine their impact, Z. noltei individuals were collected in a pollution-free site and transferred to the laboratory in seawater microcosms. This dwarf eelgrass was exposed to a pesticide cocktail and copper, alone or simultaneously, at temperatures (10 °C, 20 °C, 28 °C) representative of different seasons. After a two-week contamination, leaf growth, leaf bioaccumulation of Cu, and differential expression of target genes were studied. Eelgrasses bioaccumulated Cu...
Tipo: Text Palavras-chave: Zostera noltei; Arcachon Bay; Pesticides; Copper; Cellular impacts.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00407/51831/58536.pdf
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Chronic dietary exposure of zebrafish to PAH mixtures results in carcinogenic but not genotoxic effects ArchiMer
Larcher, Thibaut; Perrichon, Prescilla; Vignet, Caroline; Ledevin, Mireille; Le Menach, Karyn; Lyphout, Laura; Landi, Laure; Clerandeau, Christelle; Lebihanic, F.; Menard, Dominique; Burgeot, Thierry; Budzinski, Helene; Akcha, Farida; Cachot, J.; Cousin, Xavier.
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous contaminants that can be present at high levels as mixtures in polluted aquatic environments. Many PAHs are potent mutagens and several are well-known carcinogens. Despite numerous studies on individual compounds, little is known about the toxicity of PAHs mixtures that are encountered in environmental situations. In the present work, zebrafish were continuously fed from 5 days post-fertilisation to 14 months post-fertilisation (mpf) with a diet spiked with fractions of either pyrolytic (PY), petrogenic light oil (LO), or petrogenic heavy oil (HO) origin at three concentrations. A decrease in survival was identified after 3 mpf in fish fed with the highest concentration of HO or LO, but not for PY. All...
Tipo: Text Palavras-chave: Polycyclic aromatic hydrocarbon; Zebrafish; Carcinogenesis; Genotoxicity; Neoplasia; Carcinoma; Toxicological pathology.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00242/35329/34108.pdf
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Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth ArchiMer
Vignet, Caroline; Le Menach, Karyn; Mazurais, David; Lucas, Julie; Perrichon, Prescilla; Le Bihanic, Florane; Devier, Marie-hélène; Lyphout, Laura; Frere, Laura; Begout, Marie-laure; Zambonino-infante, Jose Luis; Budzinski, Helene; Cousin, Xavier.
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel...
Tipo: Text Palavras-chave: Danio rerio; Life cycle; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Alkylated PAH; Jaw; Digestion.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29907/28490.pdf
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Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish—part II: behavior ArchiMer
Vignet, Caroline; Le Menach, Karyn; Lyphout, Laura; Guionnet, Tiphaine; Frere, Laura; Leguay, Didier; Budzinski, Helene; Cousin, Xavier; Begout, Marie-laure.
In the last 10 years, behavior assessment has been developed as an indicator of neurotoxicity and an integrated indicator of physiological disruption. Polycyclic aromatic hydrocarbon (PAH) release into the environment has increased in recent decades resulting in high concentrations of these compounds in the sediment of contaminated areas. We evaluated the behavioral consequences of long-term chronic exposure to PAHs, by exposing zebrafish to diets spiked with three PAH fractions at environmentally relevant concentrations. Fish were exposed to these chemicals from their first meal (5 days postfertilization) until they became reproducing adults (at 6 months old). The fractions used were representative of PAHs of pyrolytic (PY) origin and of two oils...
Tipo: Text Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Heavy oil; Light crude oil; Anxiety-like behavior; Photomotor responses; Maze; Novel tank.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29910/28380.pdf
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Combined effects of antifouling biocides on the growth of three marine microalgal species ArchiMer
Dupraz, Valentin; Stachowski-haberkorn, Sabine; Menard, Dominique; Limon, Gwendolina; Akcha, Farida; Budzinski, Helene; Cedergreen, Nina.
The toxicity of the antifouling compounds diuron, irgarol, zinc pyrithione (ZnPT), copper pyrithione (CuPT) and copper was tested on the three marine microalgae Tisochrysis lutea, Skeletonema marinoi and Tetraselmis suecica. Toxicity tests based on the inhibition of growth rate after 96-h exposure were run using microplates. Chemical analyses were performed to validate the exposure concentrations and the stability of the compounds under test conditions. Single chemicals exhibited varying toxicity depending on the species, irgarol being the most toxic chemical and Cu the least toxic. Selected binary mixtures were tested and the resulting interactions were analyzed using two distinct concentration-response surface models: one using the concentration addition...
Tipo: Text Palavras-chave: Diuron; Irgarol; Pyrithione; Copper; Microbial ecotoxicology; Mixture model.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00445/55695/57362.pdf
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Contribution au développement et à l'amélioration des techniques d'échantillonnage passif (DGT et POCIS) : développment et amélioration techniques EP : II-B-01 (16). Rapport final ArchiMer
Gonzalez, Jean-louis; Munaron, Dominique; Podeur, Christian; Tapie, Nathalie; Budzinski, Helene; Marusczak, Nicolas; Montero, Natalia; Belzunce-segarra, Maria Jesus.
This project is a contribution to the improvement and development of passive sampling techniques. The objectives are: - to develop ways to limit the fouling formation on the samplers requiring prolonged immersion time (POCIS) and ensure a "constant" and reproducible sampling rate - test and validate the DGT developed specifically for Hg wich is a "WFD priority" metal. Key words (thematic and geographical area) :Passive samplers, POCIS, DGT, fouling, sampling rate, Hg, baie du Lazaret , étang de Thau
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00115/22628/20353.pdf
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Définition des réseaux de surveillance DCE de la qualité des masses d’eau côtières de l’île de Mayotte - Rapport final Tome 1 Synthèse et propositions ArchiMer
Turquet, Jean; Nicet, Jean Benoit; Cambert, Harold; Denis, Yves; Gonzalez, Jean-louis; Bigot, Lionel; Guyomarch, Julien; Budzinski, Helene; Tapie, Nathalie; Jamon, Alban; Pribat, B.
Les prochaines échéances imposées par le calendrier de la Directive Cadre sur l’Eau sur les eaux côtières visent à atteindre ou à restaurer le bon état qualitatif et quantitatif de toutes les masses d’eau d'ici l'horizon 2015. Ces objectifs nécessitent la définition du bon état chimique des masses d’eaux, l’évaluation des tendances et la mise en place des programmes de mesures.
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00035/14621/11900.pdf
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Définition des réseaux de surveillance DCE de la qualité des masses d’eau côtières de l’île de Mayotte - Rapport final Tome 2 Cartographie des données DCE par masse d’eau ArchiMer
Turquet, Jean; Nicet, Jean Benoit; Cambert, Harold; Denis, Yves; Gonzalez, Jean-louis; Bigot, Lionel; Guyomarch, Julien; Budzinski, Helene; Tapie, Nathalie; Jamon, Alban; Pribat, B.
Le tome 2 de l‘étude de « définition des réseaux de surveillance de la qualité des masses d’eau côtières de l’île de Mayotte », présente sous forme cartographique les données bancarisées sous format MapInfo. Seules les études intéressantes vis à vis de la DCE et disponibles ont été référencées et prises en compte dans cet exercice (cf. références bibliographique du tome 2). Ce premier exercice de bancarisation, ne se veut pas exhaustif, et devra faire l’objet d’entretiens, d’ajouts réguliers.
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00035/14622/11901.pdf
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Detection of early effects of a single herbicide (diuron) and a mix of herbicides and pharmaceuticals (diuron, isoproturon, ibuprofen) on immunological parameters of Pacific oyster (Crassostrea gigas) spat ArchiMer
Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco.
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides...
Tipo: Text Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf
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Development of a reference artificial sediment for chemical testing adapted to the MELA sediment contact assay ArchiMer
Le Bihanic, Florane; Perrichon, Prescilla; Landi, Laure; Clerandeau, Christelle; Le Menach, Karyn; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome.
Most persistent organic pollutants, due to their hydrophobic properties, accumulate in aquatic sediments and represent a high risk for sediment quality. To assess the toxicity of hydrophobic pollutants, a novel approach was recently proposed as an alternative to replace, refine and reduce animal experimentation: the medaka embryo–larval sediment contact assay (MELAc). This assay is performed with Japanese medaka embryos incubated on a natural sediment spiked with the compound being tested. With the aim of improving this assay, our study developed a reference exposure protocol with an artificial sediment specifically designed to limit natural sediment composition uncertainties and preparation variability. The optimum composition of the new artificial...
Tipo: Text Palavras-chave: Artificial sediment; Spiked sediment; PAHs; Japanese medaka embryos; Embryotoxicity; Teratogenicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00187/29872/28479.pdf
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Developmental toxicity of PAH mixtures in fish early life stages. Part I: adverse effects in rainbow trout ArchiMer
Le Bihanic, Florane; Morin, Benedicte; Cousin, Xavier; Le Menach, Karyn; Budzinski, Helene; Cachot, Jerome.
A new gravel-contact assay using rainbow trout, Oncorhynchus mykiss, embryos was developed to assess the toxicity of polycyclic aromatic hydrocarbons (PAHs) and other hydrophobic compounds. Environmentally realistic exposure conditions were mimicked with a direct exposure of eyed rainbow trout embryos incubated onto chemical-spiked gravels until hatching at 10 °C. Several endpoints were recorded including survival, hatching delay, hatching success, biometry, developmental abnormalities, and DNA damage (comet and micronucleus assays). This bioassay was firstly tested with two model PAHs, fluoranthene and benzo[a]pyrene. Then, the method was applied to compare the toxicity of three PAH complex mixtures characterized by different PAH compositions: a pyrolytic...
Tipo: Text Palavras-chave: PAH mixture; Oil extract; Pyrolytic extract; Embryotoxicity; Developmental defects; Genotoxicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29919/28384.pdf
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Developmental toxicity of PAH mixtures in fish early life stages. Part II: adverse effects in Japanese medaka ArchiMer
Le Bihanic, Florane; Clerandeau, Christelle; Le Menach, Karyn; Morin, Benedicte; Budzinski, Helene; Cousin, Xavier; Cachot, Jerome.
In aquatic environments, polycyclic aromatic hydrocarbons (PAHs) mostly occur as complex mixtures, for which risk assessment remains problematic. To better understand the effects of PAH mixture toxicity on fish early life stages, this study compared the developmental toxicity of three PAH complex mixtures. These mixtures were extracted from a PAH-contaminated sediment (Seine estuary, France) and two oils (Arabian Light and Erika). For each fraction, artificial sediment was spiked at three different environmental concentrations roughly equivalent to 0.5, 4, and 10 μg total PAH g−1 dw. Japanese medaka embryos were incubated on these PAH-spiked sediments throughout their development, right up until hatching. Several endpoints were recorded at different...
Tipo: Text Palavras-chave: Developmental abnormalities; Comet assay; Micronucleus assay; Larvae locomotion; Oil extract; Pyrolytic extract; Medaka embryo-larval assay.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00188/29920/28385.pdf
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Environmental concentrations of benz[a]anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka larvae ArchiMer
Le Bihanic, Florane; Sommard, Vivien; De Lansalut, Perrine; Pichon, Anaïk; Grasset, Julie; Berrada, Saadia; Budzinski, Helene; Cousin, Xavier; Morin, Benedicte; Cachot, Jerome.
Benz[a]anthracene (BaA) is a ubiquitous polycyclic aromatic hydrocarbon found in numerous aquatic ecosystems. However, ecotoxicological data in aquatic organisms are scarce. To remedy this lack of data, Japanese medaka (Oryzias latipes) embryos were exposed to BaA and toxic effects were investigated at multiple toxicological endpoints. Japanese medaka embryos were incubated onto BaA-spiked artificial sediment for 9 days at low or moderate environmental concentrations ranging from 0.9 to 12 µg g−1 dw. BaA-exposed embryos exhibited significant tachycardia. BaA exposure was also shown to increase CYP1A activity in the hepato-biliary tissue as well as craniofacial deformities and DNA damage in pro-larvae. The photomotor response of BaA-exposed larvae was...
Tipo: Text Palavras-chave: Benz[a]anthracene; Spiked sediment; Teratogenicity; DNA damage; Swimming behavior; In vivo EROD activity.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00244/35513/34729.pdf
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Etude pilote préliminaire : Première évaluation des niveaux de contamination chimique des eaux littorales guyanaises dans le cadre de la préparation du volet "chimie" du contrôle de surveillance DCE - campagne Novembre 2008 ArchiMer
Gonzalez, Jean-louis; Tapie, Nathalie; Budzinski, Helene; Guyomarch, Julien; Crenn, Vincent; Bretaudeau-sanjuan, Jane.
L'objectif principal de cette première étude était d'évaluer, dans le contexte guyanais, l'opérationnalité de techniques d'échantillonnage et de faire une évaluation préliminaire de la contamination chimique de différentes masses d'eaux guyanaises (2 sites en eau de transition et 1 site en eau côtière). Deux types d'échantillonneurs passifs ont été mis en oeuvre: DGT (pour les métaux traces) et SBSE (pour les composés organiques hydrophobes). Les molécules hydrophiles ont été recherchées directement dans l'eau prélevée. Les mesures DGT montrent que le site de l'estuaire de Kourou se caractérise, pour tous les métaux, par des concentrations plus importantes que celles déterminées dans les deux autres stations. S'agissant de mesures ponctuelles, réalisées...
Tipo: Text Palavras-chave: Guyane; DCE; Contamination chimique; Masses d'eau; Métaux traces; Composés hydrophiles et hydrophobes; Échantillonnage passif; DGT; SBSE.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00035/14623/11902.pdf
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Exposures of zebrafish through diet to three environmentally relevant mixtures of PAHs produce behavioral disruptions in unexposed F1 and F2 descendant ArchiMer
Vignet, Caroline; Joassard, Lucette; Lyphout, Laura; Guionnet, Tiphaine; Goubeau, Manon; Le Menach, Karyn; Brion, Francois; Kah, Olivier; Chung, Bon-chu; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier.
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades. PAHs are hydrophobic molecules which can accumulate in high concentrations in sediments acting then as major secondary sources. Fish contamination can occur through contact or residence nearby sediments or though dietary exposure. In this study, we analyzed certain physiological traits in unexposed fish (F1) issued from parents (F0) exposed through diet to three PAH mixtures at similar and environmentally relevant concentrations but differing in their compositions. For each mixture, no morphological differences were observed between concentrations. An increase in locomotor activity was observed in larvae issued from fish...
Tipo: Text Palavras-chave: Danio rerio; Polycyclic aromatic hydrocarbon; Offspring; Transgenerational effect; Heavy oil; Light crude oil; Locomotor activity; Photomotor response; Anxiety-like behavior.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00259/37030/35688.pdf
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Fish Reproduction Is Disrupted upon Lifelong Exposure to Environmental PAHs Fractions Revealing Different Modes of Action ArchiMer
Vignet, Caroline; Larcher, Thibaut; Davail, Blandine; Joassard, Lucette; Le Menach, Karyn; Guionnet, Tiphaine; Lyphout, Laura; Ledevin, Mireille; Goubeau, Manon; Budzinski, Helene; Begout, Marie-laure; Cousin, Xavier.
Polycyclic aromatic hydrocarbons (PAHs) constitute a large family of organic pollutants emitted in the environment as complex mixtures, the compositions of which depend on origin. Among a wide range of physiological defects, PAHs are suspected to be involved in disruption of reproduction. In an aquatic environment, the trophic route is an important source of chronic exposure to PAHs. Here, we performed trophic exposure of zebrafish to three fractions of different origin, one pyrolytic and two petrogenic. Produced diets contained PAHs at environmental concentrations. Reproductive traits were analyzed at individual, tissue and molecular levels. Reproductive success and cumulative eggs number were disrupted after exposure to all three fractions, albeit to...
Tipo: Text Palavras-chave: Polycyclic aromatic hydrocarbons; Zebrafish; Spawning success; Fertilization; Gonad differentiation; Molecular mechanisms.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00355/46641/46465.pdf
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