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Distribution and genetic diversity of tomato-infecting begomoviruses in Brazil. Repositório Alice
RIBEIRO, S. G.; AMBROZEVÍCIUS, L. P.; ÁVILA, A. C.; BEZERRA, I. C.; CALEGARIO, R. F.; FERNANDES, J. J.; LIMA, M. F.; MLLO, R. N. de; ROCHA, H.; ZERBINI, F. M..
Tomato-infecting begomoviruses have been reported throughout Brazil since the introduction of the B biotype of Bemisia tabaci. Here, we report a large scale survey on the distribution and genetic diversity of tomato-infecting begomoviruses. Tomato samples withtypical begomovirus symptoms were collected in seven different states, comprising the major tomato growing areas of the country. Viruses were detected by polymerase chain reaction (PCR) using universal primers for the genus Begomovirus. PCR-amplified fragments were cloned and sequenced. Based on sequence comparisons and phylogenetic analyses, at least seven previously undescribed species of begomoviruses were found. Four of the new viruses were found exclusively in the Southeastern states, two...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Diversidade genética; Geminivirus; Tomato; Tomate; Doença; Vírus; Begomovirus.
Ano: 2003 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/155962
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Identificatio brugmansia saveolens mottle virus in brugnansia SP. Repositório Alice
SOUZA, T. A.; INOUE-NAGATA, A. K.; CALEGARIO, R. F.; KITAJIMA, E. W..
2016
Tipo: Resumo em anais de congresso (ALICE) Palavras-chave: Planta ornamental; Virus.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/handle/doc/1066097
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In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). Repositório Alice
CALEGARIO, R. F.; LABATE, M.T.V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W..
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Citrus; Citrus leprosis virus C; Brevipalpus phoenicis; Cilevirus; Citrus sinensis; Polyclonal antibody; Serology.
Ano: 2012 URL: http://www.alice.cnptia.embrapa.br/handle/doc/937928
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