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Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana. Repositório Alice
CANGAHUALA-INOCENTE, G.C.; STEINER, N.; MALDONADO, S.B.; GUERRA, M.P..
The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid?Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the...
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Total sugars; Starch; Pineapple guava; Metabolism; Açúcares totais; Amido; Goiabeira serrana; Metabolismo.
Ano: 2009 URL: http://www.alice.cnptia.embrapa.br/handle/doc/125724
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