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Influence of substrates and in vitro preconditioning treatments on ex vitro acclimatization of Arachis retusa. Repositório Alice
PACHECO, G.; GAGLIARDI, R.F.; COGLIATTI, M.B.; MANHÃES, H.B.; CARNEIRO, L.A.; VALLS, J.F.M.; MANSUR, E..
The objective of this work was to evaluate the influence of substrate and preconditioning treatments on the acclimatization of in vitro plants of Arachis retusa. Plants were transferred to Plantmax or sand, and fertilized with Hoagland's nutrient solution. Plants maintained in sand, with or without fertilizer, showed the highest survival rates. In order to evaluate the influence of in vitro preconditioning treatments, stem segments were cultured on MS medium supplemented with different sucrose concentrations. The highest survival and developmental rates were observed in plants from two accessions cultured on MS supplemented with 1.5% and 3% sucrose. Flowering and fruit production were observed after five months.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Arachis; Groundnut; In vitro conservation; Micropropagation; Amendoim; Conservação in vitro; Micropropagação.
Ano: 2006 URL: http://www.alice.cnptia.embrapa.br/handle/doc/118384
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Rescue of a non-viable accession and rapd analysis of recovered plants of Arachis retusa. Repositório Alice
GAGLIARDI, R.F.; PACHECO, G.P.; OLIVEIRA, C.A.; CARNEIRO, L.A.; VALLS, J.F.M.; VIEIRA, M.L.C.; MANSUR, E..
In vitro regeneration of Arachis retusa was examined for the purpose of germplasm renewal and conservation. Random amplified polymorphic DNA (RAPD) fingerprinting was used to evaluate the genetic stability of plants derived from embryo axes and apical segments. Ten arbitrary decamer primers were screened and five of them were selected. Ninety genomic regions were evaluated, with an average of 18 loci per clone. All amplified segments were monomorphic. The results indicate that recovered plants are genetically stable at the assessed genomic regions and that both regeneration processes are suitable for in vitro germplasm preservation of Arachis species.
Tipo: Artigo em periódico indexado (ALICE) Palavras-chave: Germplasm conservation; Micropropagation; Molecular characterization; Genetic stability; Conservação de germoplasma; Micropropagação; Caracterização molecular; Estabilidade genética.
Ano: 2004 URL: http://www.alice.cnptia.embrapa.br/handle/doc/110084
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