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Diversity of cultured photosynthetic flagellates in the northeast Pacific and Arctic Oceans in summer ArchiMer
Balzano, S.; Gourvil, P.; Siano, Raffaele; Chanoine, M.; Marie, D.; Lessard, S.; Sarno, D.; Vaulot, Daniel.
During the MALINA cruise (summer 2009), an extensive effort was undertaken to isolate phytoplankton strains from the northeast (NE) Pacific Ocean, the Bering Strait, the Chukchi Sea, and the Beaufort Sea. In order to characterise the main photosynthetic microorganisms occurring in the Arctic during the summer season, strains were isolated by flow cytometry sorting (FCS) and single cell pipetting before or after phytoplankton enrichment of seawater samples. Strains were isolated both onboard and back in the laboratory and cultured at 4 °C under light/dark conditions. Overall, we isolated and characterised by light microscopy and 18 S rRNA gene sequencing 104 strains of photosynthetic flagellates which grouped into 21 genotypes (defined by 99.5% 18 S rRNA...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00114/22491/20172.pdf
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