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CATALYTIC POTENTIAL OF SOIL HYDROLASES IN NORTHEAST CHINA UNDER DIFFERENT SOIL MOISTURE CONDITIONS R.C. Suelo Nutr. Veg.
Zhang,Y.L; Sun,Cc.X; Chen,L.J; Duan,Z.H.
An incubation test with black soil (Phaeozem), Albic soil (Albic Luvisols), brown soil (Cambisols), and cinnamon soil (Chromic Luvisol) from Northeast China was conducted under the conditions of 10%, 20% and30 % field capacity, and the kinetic parameters of soil urease, phosphatase, and arylsulphatase were determined, aimed to study the changes in the catalytic potential of these enzymes under different soil moisture conditions. All test enzymes exhibited typical Michaelis-Menten kinetic behaviors. The test enzymes exhibited the highest enzyme-substrate affinity (l/Km) at 20% or 30% field capacity. With increasing soil moisture content, the Fmax of test soil urease decreased, while that of soil phosphatase and arylsulphatase increased, with the maximum...
Tipo: Journal article Palavras-chave: Soil enzymatic kinetic parameters; Soil hydrolase; Soil moisture condition.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912009000200003
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KINETIC AND THERMODYNAMIC BEHAVIORS OF SOIL UREASE AS AFFECTED BY UREASE INHIBITORS R.C. Suelo Nutr. Veg.
Juan,Y.H; Chen,Z.H; Chen,L.J; Wu,Z.J; Wang,R; Sun,W.T; Zhang,Y.L.
The kinetic characteristics of soil urease have attracted great attention, but little information was available on its kinetic and thermodynamic behaviors as affected by urease inhibitors and temperature. With black soil (Pachic Udic Mollisol) in Heilongjiang Province of Northeast China as a test soil, an incubation test was conducted to investigate the effect of urease inhibitors (hydroquinone, HQ; phenyl phosphorodiamidate, PPD; N-(n-Butyl) thiophosphoric triamide, NBPT) on kinetic and thermodynamic behaviors of soil urease at 10°C, 20°C and 30°C. The results showed that all tested inhibitors increased Km and decreased Vmax, behaving as mixed inhibitors to soil urease. With increasing temperature, the Km and Vmax values increased significantly. With...
Tipo: Journal article Palavras-chave: Meterkinetic paras; Thermodynamic parameters; Soil urease; Urease inhibitors; Temperatures.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912010000100001
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KINETICS OF SOIL UREASE AFFECTED BY UREASE INHIBITORS AT CONTRASTING MOISTURE REGIMES R.C. Suelo Nutr. Veg.
Juan,Y.H; Chen,L.J; Wu,Z.J; Wang,R.
With black soil (Pachic Udic Argiboroll) of Northeastern China as the test object, an incubation test was conducted to investigate the effects of urease inhibitors, hydroquinone (HQ), phenyl phosphorodiamidate (PPD) and N-(n-Butyl) thiophosphoric triamide (NBPT), on the kinetic characteristics of soil urease under normal moisture and waterlogged conditions, aimed to study the changes of catalytic potential of soil urease and the inhibition mechanisms. The results showed that test urease exhibited typical Michaelis-Menten kinetic behaviors, and all test inhibitors increased the Km and decreased the Vmax of soil urease, behaving as mixed inhibitors to soil urease. Under both normal and waterlogged conditions, compared with HQ, PPD and NBPT made Km increase...
Tipo: Journal article Palavras-chave: Kinetic parameters; Mixed inhibition; Moisture regime; Soil urease.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912009000200004
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SOIL NUTRIENT CONTENTS AND ENZYMATIC CHARACTERISTICS AS AFFECTED BY 7-YEAR NO TILLAGE UNDER MAIZE CROPPING IN A MEADOW BROWN SOIL R.C. Suelo Nutr. Veg.
Zhang,Y.L; Chen,L.J; Chen,Z.H; Sun,C.X; Wu,Z.J; Tang,X.H.
No tillage is being populanzed for the rainfed maize production in Northeast China. In order to evalúate its effects on the nutrient contents and enzymatic charactenstics in upland soils of Northeast China, surface (0-20 cm) meadow brown soil samples were collected from the plots under no tillage and conventional tillage in a 7-year field experiment under maize cropping in Shenyang, with the soil pH, contents of total C, N, P and S and available N, activities of α- and (β-galactosidase, α- and (β-glucosidase, urease, protease, phosphomonoesterase, phosphodiesterase, and arylsulphatase, and kinetic parameters of (β-glucosidase, protease, phosphomonoesterase, phosphodiesterase, and arylsulphatase determined....
Tipo: Journal article Palavras-chave: Conservation tillage; Conventional tillage; Soil enzyme activity; Enzyme kinetic properties.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912010000200006
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