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Registros recuperados: 6
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Characterization and PCR multiplexing of polymorphic microsatellite loci for the invasive ant Wasmannia auropunctata Inra
Fournier, D.; Foucaud, J.; Loiseau, A.; Cros-Arteil, S.; Jourdan, H.; Orivel, J.; Le Breton, J.; Chazeau, J.; Dejean, A.; Keller, L.; Estoup, A..
Highly polymorphic genetic markers provide a useful tool for estimating genetic parametersin studies of the evolution of sociality in insects. We isolated and characterized 12 polymorphicmicrosatellite loci in the invasive ant, Wasmannia auropunctata, and described experimental conditions for PCR (polymerase chain reaction) multiplexing and simultaneously genotyping these loci in two sets of five and seven markers. The number of alleles per locus ranged from two to 14 and the observed heterozygosity ranged from 0.233 to 0.967. Moreover, results of cross-species amplification tests are reported in three other species of Wasmannia and in two species of the genus Allomerus
Tipo: Journal Article Palavras-chave: STRUCTURE GENETIQUE; POLYMORPHISME ANTS; BLEPHARIDATTINI; ESPECE ENVAHISSANTE; MICROSATELLITES; MYRMICINAE; PEST.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20074ea97322&uri=/notices/prodinra1/2007/08/
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Differential gene expression of the honey bee Apis mellifera associated with Varroa destructor infection Inra
Navajas, M.; Migeon, A.; Alaux, C.; Martin-Magniette, M.L.; Robinson, G.E.; Evans, J.D.; Cros-Arteil, S.; Crauser, D.; Le Conte, Y..
Background: The parasitic mite, Varroa destructor, is the most serious pest of the western honey bee, Apis mellifera, and has caused the death of millions of colonies worldwide. This mite reproduces in brood cells and parasitizes immature and adult bees. We investigated whether Varroa infestation induces changes in Apis mellifera gene expression, and whether there are genotypic differences that affect gene expression relevant to the bee's tolerance, as first steps toward unravelling mechanisms of host response and differences in susceptibility to Varroa parasitism. Results: We explored the transcriptional response to mite parasitism in two genetic stocks of A. mellifera which differ in susceptibility to Varroa, comparing parasitized and non-parasitized...
Tipo: Journal Article Palavras-chave: ABEILLE DOMESTIQUE; APIS MELLIFERA; ACARIEN PARAISITE; VARROA DESTRUCTOR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20095dd5dfa6&uri=/notices/prodinra1/2010/12/
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First record of the invasive mite Tetranychus evansi (Acari: Tetranychidae) Baker and Pritchard in Greece Inra
Tsagkarakou, A.; Cros-Arteil, S.; Navajas Navarro, M..
The red spider mite Tetranychus evansi (Acari : Tetranychidae) Baker and Pritchard was recorded for the first time in Greece, in the area of Tympaki (south-central Crete) on Solanum nigrum. T evansi is a pest of crops of the family Solanaceae, which are grown extensively in Crete. The species identification was based on both morphological and molecular data. The second internal transcribed spacer was PCR amplified and sequenced in samples from Crete. Sequences were compared with the sequence of T evansi from Brazil and with the ITS2 sequences (retrieved from GenBank) of the two closely related tetranychid species most commonly found in Greece, Tetranychus turkestani and Tetranychus urticae.Author Keywords: internal transcribed spacer (ITS2); red spider...
Tipo: Journal Article Palavras-chave: SOLANACEES; IDENTIFICATION INTERNAL TRANSCRIBED SPACER (ITS2); RED SPIDER MITE; SOLANACEAE; SOLANUM NIGRUM; TETRANYCHIDAE; TETRANYCHUS TURKESTANI; TETRANYCHUS URTICAE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20093142d41b&uri=/notices/prodinra1/2010/03/
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Microsatellite markers reveal spatial genetic structure of Tetranychus urticae (Acari: Tetranychidae) populations along a latitudinal gradient in Europe Inra
Carbonnelle, S.; Hance, T.; Migeon, A.; Baret, P.; Cros-Arteil, S.; Navajas, M..
The genetic structure of populations of the two-spotted spider mite Tetranychus urticae was investigated along a south–north European transect spanning from southern France to The Netherlands. Mites were collected on Urtica dioica in 6 sampling zones. Microsatellite variation at 5 loci revealed considerable genetic variation with an average heterozygozity of 0.49. Significant heterozygote deficiency was found in 7 populations out of the 18 samples analyzed and one of them was completely monomorphic. Tetranychus urticae populations show some level of genetic structuring. First, genetic differentiation between localities (F ST estimates) was significant for all comparisons. Second, the analysis of molecular variance, AMOVA, indicates that there is an...
Tipo: Journal Article Palavras-chave: TETRANYCHUS URTICAE; TETRANYCHIDAE.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2008c49f89cb&uri=/notices/prodinra1/2008/05/
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Sequence analysis of the ribosomal internal transcribed spacers region in spider mites (Prostigmata: Tetranychidae) occurring in citrus orchards in Eastern Spain: use for species discrimination Inra
Hurtado, M.A;; Ansaloni, T.; Cros-Arteil, S.; Jacas, J.A.; Navajas Navarro, M..
Tetranychus urticae is a polyphagous mite which is an important pest of citrus worldwide. This mite can be found feeding on many plant species occurring in the citrus agrosystem moving from weeds to trees. Because field samples consist of a mixture of different Tetranychidae species, as a first step necessary to further implement population characterisation of T. urticae, species-discriminating criteria based on molecular techniques are needed. In this study, the nucleotide variation of the internal transcribed spacers (ITS) 1 and 2 and the intergenic 5.8S fragment of nuclear rDNA of T. urticae, Tetranychus turkestani, Tetranychus evansi, Tetranychus ludeni and Panonychus citri have been determined. Results demonstrate that for these species, the rDNA ITS2...
Tipo: Journal Article Palavras-chave: CITRUS RETICULATA; PANONYCHUS CITRI; TETRANYCHUS SPP.; TETRANYCHUS URTICAE; TETRANYCHUS TURKESTANI; TETRANYCHUS EVANSI; TETRANYCHUS LUDENI.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009722d6e4c&uri=/notices/prodinra1/2011/06/
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Social immunity in honeybees (Apis mellifera): transcriptome analysis of varroa-hygienic behaviour Inra
Le Conte, Y.; ALAUX, C.; Martin, J.F.; Harbo, J.R.; Harris, J.W.; Dantec, C.; Séverac, D.; Cros-Arteil, S.; Navajas Navarro, M..
Honeybees have evolved a social immunity consisting of the cooperation of individuals to decrease disease in the hive. We identified a set of genes involved in this social immunity by analysing the brain transcriptome of highly varroa-hygienic bees, who efficiently detect and remove brood infected with the Varroa destructor mite. The function of these candidate genes does not seem to support a higher olfactory sensitivity in hygienic bees, as previously hypothesized. However, comparing their genomic profile with those from other behaviours suggests a link with brood care and the highly varroa-hygienic Africanized honeybees. These results represent a firststep toward the identification of genes involved in social immunity and thus provide first insights...
Tipo: Journal Article Palavras-chave: PARASITE; INSECTE SOCIAL; IMMUNITE SOCIALE; COMPORTEMENT HYGIENIQUE; EXPRESSION GENIQUE HONEYBEE; SOCIAL IMMUNITY; HYGIENIC BEHAVIOUR; VARROA; GENE EXPRESSION.
Ano: 2011 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2011f6e141fc&uri=/notices/prodinra1/2011/06/
Registros recuperados: 6
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