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Registros recuperados: 5
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A new view on aphid resistance in melon: the role of Aphis gossypii variability National Institute of Agronomic Research
Boissot, N.; Mistral, P.; Chareyron, V.; Dogimont, C..
Resistance to Aphis gossypii in Cucumis melo has been largely studied but A. gossypii variability has never been considered. Resistance to colonization by A. gossypii, clone NM1, and to non persistent virus transmission by this clone is conferred by a NBS-LRR gene called Vat, isolated in the PI 161375 accession. We investigated resistance to A. gossypii with four clones of A. gossypii that belong to two very distinct genotypes, NM1 and C9. The Vat gene conferred a high level of resistance to a NM1 clone and partial resistance to a C9 clone. Four additive QTL and two pairs of epistatic QTL were detected in a recombinant inbred line population derived from the cross ‘Védrantais’ x PI 161375. Half of them clearly have a specific effect on the acceptance by...
Tipo: Conference Paper Palavras-chave: Cucumis melo; Vat; QTL; Insect resistance; Allelic variability; Disease resistance; Virus vector; Virus transmission; SSR markers; Aphis gossypii.
Ano: 2008 URL: http://hdl.handle.net/2174/207
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Genetic and molecular diversity of fruit traits in melon Inra
Dogimont, C..
Tipo: Conference Paper Palavras-chave: CLIMACTERIQUE; TILLING.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010a754aebf&uri=/notices/prodinra1/2010/04/
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Histological study of shoot organogenesis in melon (Cucumis melo L.) after genetic transformation National Institute of Agronomic Research
Chovelon, V.; Restier, V.; Dogimont, C.; Aarouf, J..
Leave and cotyledon explants from two melon genotypes ‘Vedrantais’ and ‘Paul’ were cultured on different media and their regeneration was followed during 60 days. High frequency of plant regeneration was observed with ‘Vedrantais’ cotyledons cultured on MS medium added with BAP and 2-iP. Using this protocol, Agrobacterium mediated transformation was developed and a significant reduction of the plant regeneration rate was observed. Explants histological analysis revealed the presence of transformed meristematic areas but a rapid and premature cell vacuolization was observed. During plant regeneration, meristematic cells were affected by important cytological damages: cell dislocation accompanied by cell death and final collapsus. This disruption of...
Tipo: Poster Palavras-chave: Cucumis melo; Agrobacterium tumefaciens; GUS uidA; Genetic transformation; Regeneration.
Ano: 2008 URL: http://hdl.handle.net/2174/285
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Host plant resistance to aphids in cultivated crops : genetic and molecular bases, and interactions with aphid populations Inra
Dogimont, C.; Bendahmane, A.; Chovelon, V.; Boissot, N..
La résistance génétiquement déterminée des plantes est un moyen efficace et respectueux de l’environnement de lutter contre les pucerons. Cependant, des biotypes de pucerons, capables de contourner les résistances, sont apparus dans plusieurs systèmes plantes–pucerons. Dans cet article, sur les bases génétiques et moléculaires des résistances aux pucerons chez les espèces cultivées, nous insistons sur le nombre de gènes de résistance aux pucerons disponibles est limité. Les résistances aux pucerons sont contrôlées par des gènes majeurs (dominants ou récessifs) aussi bien que par des facteurs génétiques quantitatifs. Deux gènes de résistance aux pucerons ont été clonés jusqu’à présent, chez la tomate et le melon. Ils codent pour des protéines NBS-LRR, qui...
Tipo: Journal Article Palavras-chave: GENE D'AVIRULENCE; GENE CLONE; HEREDITE; RESSOURCE GENETIQUE; SPECIFICITE DE RESISTANCE HEMIPTERA; APHIDIDAE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20108eda9506&uri=/notices/prodinra1/2010/07/
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Molecular diversity at the Vat/Pm-W resistance locus in melon National Institute of Agronomic Research
Dogimont, C.; Chovelon, V.; Tual, S.; Boissot, N.; Rittener-Rüff, V.; Giovinazzo, N.; Bendahmane, A..
The resistance Vat gene of melon was isolated by map-based cloning. It encodes a protein with a coiled coil domain (CC), a nucleotide binding site (NBS) and leucine–rich repeats (LRR). Whereas most resistance genes confer resistance to a single pathogen, two alleles with different resistance specificities were identified at the locus. The Vat allele confers resistance to plant colonization by the aphid Aphis gossypii and to transmission of unrelated viruses by this vector specifically; the Pm-W allele confers resistance to the powdery mildew Podosphaera xanthii. Here we identified Vat-analogs in several melon genotypes using Long Range PCR, cloning and sequencing of full-length fragments. Several sources of variability were identified: (i) a different...
Tipo: Conference Paper Palavras-chave: Aphid resistance; Aphis gossypii; Powdery mildew resistance; Podosphaera xanthii; NBS-LRR; Disease resistance; Cucumis melo; Virus vector; PCR; Cloning; Sequencing; Molecular diversity.
Ano: 2008 URL: http://hdl.handle.net/2174/211
Registros recuperados: 5
Primeira ... 1 ... Última
 

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