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Registros recuperados: 12
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Application of staining techniques to improve the viability assessment of turbot (Psetta maxima) ova ArchiMer
Omnes, Marie-helene; Dorange, Germaine; Suquet, Marc; Normant, Yvon.
Staining-dye procedures were tested on the turbot ovule in order to develop a rapid technique for determining ovule viability. Neutral red and Trypan blue dyes provided better assessment of the proportion of viable or dead female gametes when inducing fertilisation.
Tipo: Text
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00002/11323/7860.pdf
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Cultured heart cells from oyster: an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin ArchiMer
Droguet, Mickael; Devauchelle, Nicole; Pennec, Jean-pierre; Quinn, Brian; Dorange, Germaine.
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10(-6) M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4,...
Tipo: Text Palavras-chave: Oyster; Heart cells; Flow cytometry; Tributyltin; Toxicity; Antifouling.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00089/20032/17688.pdf
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Cycles saisonniers, reproduction et qualite des ovocytes chez Pecten maximus en rade de Brest ArchiMer
Paulet, Yves-marie; Bekhadra, Farida; Devauchelle, Nicole; Donval, Anne; Dorange, Germaine.
This work is part of the program, "recruitment determinism in scallops" initiated in 1992 by the "Contrat de Baie". The aim of this research was to explain interannual abundance fluctuations observed for Pecten maximus, in the Bay of Brest. To this end an analytical approach to the life cycle of the species was undertaken to determine its critical phases. A phase may be considered as "critical" if the mortality rate at its level varies from one year to the other. Using data from 1989 to 1994, a precise time-table of the "physiological year" of the scallop in the Bay of Brest was determined. Fecundity and gamete production kinetics were estimated from the annual variation in the weight indices of the gonad. In the laboratory, egg quality was estimated...
Tipo: Text Palavras-chave: Pecten maximus; Recruitment; Reproductive cycle; Digestion.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15495/12861.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - Final Report 4th January 1999 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Little information is available on viral infections that affect bivalve molluscs. Such a lack of data is due ta a certain inadequacy of the diagnosis methods that are employed when massive mortality events occur. Most laboratories involved in mollusc pathology still analyse samples through light microscopy. World-wide, there is thus currently a lack of information concerning the occurrence of bivalve herpesvituses. This is probably due to the lack of suitable diagnostic tools. The basic method for identification and examination of suspect samples is predominantly histopathology. This enables the identification of any cellular changes, but is not conclusive identification of bivalve herpesviruses. This technique doesn't allow, by itself, to detect viruses...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15525/12912.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" - First Periodic Progress Report 4th January 1999 to 3rd January 2000 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure. 2 - Comparing oyster herpes-like viruses with other viruses belonging to the Helpesviridae family on the basis of sequence data and...
Tipo: Text Palavras-chave: Pathology; Diagnosis; Oyster; Herpes like virus; Herpesviridae; Immunology; Detection; Virus; Bivalves.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00044/15523/12910.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular immunological and cellular tools "VINO". Second periodic progress report: 4th January 2000 at 3rd January 2001 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in bivalves and to validate these reagents by using them in different European laboratories involved in shellfish epidemiological surveys. This will done using teclmiques developed by the patiners to characterise viruses by studying their genome and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are: 1 - Obtaining the complete oyster herpesvirus (OsHV-1) DNA sequence with determination of the genome structure. 2 - Comparing OsHV -1 with viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure. 3 -...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Validation molecular; Detection; Herpesviridae; OsHV-1; Diagnostic techniques; Oysters.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/00048/15947/13377.pdf
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Diagnosis of oyster herpes-like virus: development and validation of molecular, immunological and cellular tools "VINO" Third Periodic Progress Report : 4th January 2001 to 3rd January 2002 ArchiMer
Renault, Tristan; Davison, Andrew; Xhonneux, Florence; Dorange, Germaine; Culloty, Sarah; Novoa, Beatriz; Dixon, Peter.
Herpesvirus infections have been reported in different bivalves species around the world. Specific diagnostic tools are needed in order ta assess the causative raIe of these agents in mortalities observed among bivalves There is currently a lack of information concerning the occurrence of bivalve herpesviruses. This is probably due ta the lack of suitable diagnostic tools. The basic method for examination of bivalve samples is predominantly histology. This enables the identification of any cellular changes, but is not conclusive for virus identification. Transmission electron microscopy is a necessity for visual confirmation of viral infections. However, histology and electron microscopy are time consuming and inadequate for epidemiological studies. The...
Tipo: Text Palavras-chave: Pathology; Virology; Diagnosis; Herpes like virus; Virus; Oyster; Detection; OsHV-1; Herpesviridae.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00044/15524/12911.pdf
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Etude expérimentale de l'effet de pesticides utilises sur les bassins versants de la rade de Brest (diuron, MCPA, glyphosate) sur le développement embryonnaire et larvaire de la coquille Saint-Jacques Pecten maximus - Validation de la méthode SIRIS en milieu marin ArchiMer
Larvor-cario, Hélène; Philip De Laborie, Loic; Hureau, Dorothee; Muzellee, Marie-louise; Dorange, Germaine; Durand, Gaël; Devauchelle, Nicole.
La coquille Saint-Jacques est un bivalve largement exploité sur les côtes françaises. En Rade de Brest, cette espèce présente un intérêt commercial tout particulier du fait de présenter une noix (muscle adducteur) et un corail (organe reproducteur) bien développés toute l'année. Elle fait l'objet d'une pêcherie spécifique et très contrôlée. Durant la phase préparatoire du contrat de Baie de la Rade de Brest, des travaux financés par le programme Rade ont été menés à l'écloserie du Tinduff dans le but d'améliorer le soutien au stock naturel exploité par cette pêcherie. Malgré de nettes avancées dans la production de cette éc1oserie, des mortalités de larves constatées dans les élevages restent inexpliquées. D'autre part, des études menées par l'UBO et...
Tipo: Text
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00043/15463/12838.pdf
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La densite, un critere de qualite applicable en ecloserie aux embryos de Pecten maximus (mollusque bivalve) ArchiMer
Faure, Catherine; Devauchelle, Nicole; Dorange, Germaine.
The quality of the scallop embryos is determined by the spawners characteristics as well as by the external factors they support during the maturation and the spawning phases. All these parameters are not still well controlled during the artificial conditioning in the hatcheries. As a consequence, the quality of the embryos is drastically variable and with them, the larvae performances in terms of survival and growth rates. It would be interesting to get a simple and quick test which would allow the estimation of the spawns quality. The embryos density, evaluated by isopycnic centrifugation of Percoll medium, is well adapted to this objective. Actually, a centrifugation of embryos two hours after fertilization separates them in 3 stratum with their density...
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Quality; Survival; Larvae; Growth; Sexual maturity; Spawning populations; Spawning; Embryos; Hatcheries.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-915.pdf
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Les gamettes de Pecten maximus L. (Mollusca, bivalvia) ArchiMer
Dorange, Germaine.
L'environnement gonadique des cellules sexuelles, le déroulement de la gamétogenèse, le devenir des gamètes formés et leur qualité au moment de l'émission ont été étudiés chez Pecten maximus sur le plan ultrastructural et histochimique. Les résultats obtenus sur la gamétogenèse se sont révélés, dans l'ensemble, conformes à ceux de travaux antérieurs sur les bivalves et en outre ils ont permis: - de mettre en évidence des cellules actif dans les processus de maturation femelle; somatiques auxiliaires et leur rôle des lignées germinales mâle et - de noter l'apparition précoce, au cours de la vitellogenèse, d'inclusions de type lysosomal - d'identifier les granules corticaux et de mettre en évidence leur rôle dans la constitution d'une membrane de...
Tipo: Text
Ano: 1989 URL: http://archimer.ifremer.fr/doc/00033/14471/11768.pdf
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Organization of the genital tract of the japanese oyster, Crassostrea gigas ArchiMer
Devauchelle, Nicole; Boussaid, Béchir; Le Pennec, Gael; Philip De Laborie, Loic; Salaun, Gilles; Dorange, Germaine.
Tipo: Text
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00049/16025/13486.pdf
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Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species ArchiMer
Suquet, Marc; Billard, Roland; Cosson, Jacky; Dorange, Germaine; Chauvaud, Laurent; Mugnier, Chantal; Fauvel, Christian.
This paper presents some features of turbot sperm and compares them with data reported in the literature on other freshwater and marine fish. In captivity, turbot spermiation period lasts 6 months. When compared to other fish species, turbot males are characterized by low values of maximum gonadosomatic index (0.6-0.8%) and are poor sperm producers in terms of volume (0.2-2.2 ml), concentration (0.7-11.0 x 10(9) spermatozoa/ml) and total sperm number released per stripping (0.2-12.0 x 10(9) spermatozoa). Compared to most freshwater species, the duration of cell movement is long (1-17 min). The spermatozoon of turbot can be considered to be of a primitive type. A very high protein content (8.8 mg/ml) is found in the seminal fluid of turbot. Increasing...
Tipo: Text Palavras-chave: Scophthalmus maximus; Turbot; Biologie du sperme; Liquide séminal; Mobilité du spermatozoïde; Morphologie du spermatozoïde; Poisson marin; Scophthalmus maximus; Turbot; Sperm biology; Seminal fluid; Sperm motility; Sperm morphology; Marine fish.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00188/29893/28334.pdf
Registros recuperados: 12
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