Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 17
Primeira ... 1 ... Última
Imagem não selecionada

Imprime registro no formato completo
Bioactive Compounds Produced by Pseudoalteromonas Affect Marine Biofilm Formation ArchiMer
Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Bacterial biofilms, frequently in association with algae, protozoa and fungi, are found on all submerged structures in the marine environment. Biofilms are responsible for a range of surface‐associated and diffusible signals, which may moderate the settling behaviour of cells, spores and larvae. Thus, marine microorganisms are a new source of bioactive compounds, which enhance or inhibit the settlement of organisms.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00089/20005/17667.pdf
Imagem não selecionada

Imprime registro no formato completo
Cryopreservation of sea bass (Dicentrarchus labrax) spermatozoa in experimental and production simulating conditions ArchiMer
Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno.
A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied....
Tipo: Text Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf
Imagem não selecionada

Imprime registro no formato completo
Cryopreservation of sperm in marine fish ArchiMer
Suquet, Marc; Dreanno, Catherine; Fauvel, Christian; Cosson, J; Billard, R.
Since the first work of Blaxter in 1953, fish sperm cryopreservation has been attempted on about 30 marine species. The present paper reviews the techniques used and the results published in these species. Particular attention is paid to the handling procedure of sperm before freezing, the problems of semen ageing and semen contamination with urine. The quality of frozen-thawed semen was evaluated using previously standardized biotests, such as a two-step motility activation technique adapted for the different species and fertilization assays using a discriminating insemination technique. Most extenders used in marine fish are saline or sugar solutions. From the investigated cryoprotectants, dimethyl sulphoxide (DMSO) generally leads to the best results....
Tipo: Text Palavras-chave: DMSO; Insemination; Fertilization; Cryopreservation; Fish sperm.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-611.pdf
Imagem não selecionada

Imprime registro no formato completo
Direct and fast detection of Alexandrium minutum algae by using high frequency microbalance ArchiMer
Sousa, Celia; Compere, Chantal; Dreanno, Catherine; Crassous, Marie-pierre; Gas, Fabienne; Baus, Beatrice; Perrot, Hubert.
In this paper, a simple detection of a toxic algae, Alexandrium minutum, was developed using highly sensitive quartz crystal microbalance. In terms of performance, compared with other conventional analytical tools, the main interest of our immunosensor is based on a fast and direct detection of these living cells. This system requires the use of one monoclonal antibody directed against the surface antigen of A. minutum. We demonstrate that the whole living and motile algae are caught and detected. The high specificity of the biosensor is also demonstrated by testing several other dinoflagellate species. The frequency shift is correlated to the A. minutum cells concentration. This simple system is potentially promising for environmental monitoring purposes.
Tipo: Text Palavras-chave: Alexandrium minutum; Quartz microbalance; Microalgae; Immunosensor.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00194/30492/28897.pdf
Imagem não selecionada

Imprime registro no formato completo
DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant? ArchiMer
Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R..
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and...
Tipo: Text Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf
Imagem não selecionada

Imprime registro no formato completo
Étude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest ArchiMer
Chapelle, Annie; Le Bec, Claude; Amzil, Zouher; Dreanno, Catherine; Guillou, Laure; Klouch, Khadidja; Labry, Claire; Le Gac, Mickael; Pineau-guillou, Lucia; Siano, Raffaele; Abernot, Chantal; Andrieux, Francoise; Caradec, Florian; Destombe, Christophe; Dia, A.; Doner, Anne; Duval, Julia; Gouriou, Jeremie; Lazure, Pascal; Le Brun, Luc; Le Gal, Dominique; Malestroit, Pascale; Petton, Sebastien; Plus, Martin; Quere, Julien; Savar, Veronique; Schmitt, Sophie; Terre, A.; Youenou, Agnes.
Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été élaboré avec la Région Bretagne dans le cadre du développement du port de Brest. L’Ifremer a organisé un échantillonnage dans l’estuaire de la rivière de Daoulas, zone la plus touchée en 2012, au cours des printemps-été 2013 et 2014. Un suivi de la présence d’A. minutum dans les sédiments a également été réalisé en hiver 2014. De ces suivis, des mesures réalisées au laboratoire à ce jour, des simulations du modèle Prévimer ainsi que des résultats du réseau Rephy et Velyger, il ressort que: * La rade de Brest peut aujourd’hui être considérée comme une zone à risque pour Alexandrium minutum. En effet, depuis 2012...
Tipo: Text
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00254/36487/35029.pdf
Imagem não selecionada

Imprime registro no formato completo
Etude sur la prolifération de la micro algue Alexandrium minutum en rade de Brest. Projet Daoulex. Rapport d’avancement n° 1 : Novembre 2013 ArchiMer
Chapelle, Annie; Le Bec, Claude; Le Gac, Mickael; Labry, Claire; Amzil, Zouher; Guillou, Laure; Dreanno, Catherine; Pineau-guillou, Lucia.
Suite à l’événement de grande ampleur d’efflorescence d’Alexandrium minutum et des toxicités associées en Rade de Brest en 2012, un projet a été monté avec la Région dans le cadre du développement du port de Brest. De juillet à août 2013, l’Ifremer a organisé un échantillonnage serré dans l’estuaire de Daoulas, zone la plus touchée en 2012. De ce suivi et des mesures réalisées au laboratoire à ce jour, ainsi que des résultats du réseau Rephy et Velyger, il ressort que : * Alexandrium minutum s’est développé à nouveau en rade de Brest en 2013 mais à des teneurs inférieures à 2012. * l’épisode de bloom et de toxicité supérieur aux seuils légaux s’est déroulé plus tard qu’en 2012, du 1er juillet jusqu’à 5 août. * les efflorescences et la toxicité ont touché...
Tipo: Text
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00191/30230/28644.pdf
Imagem não selecionada

Imprime registro no formato completo
Le projet ANR-COMANCHE 2010-STRA-010. Interactions écosystémiques et impacts anthropiques dans les populations de COquilles Saint-Jacques (Pecten maximus) de la MANCHE. Rapport scientifique final ArchiMer
Foucher, Eric; Blanchard, Michel; Cugier, Philippe; Desroy, Nicolas; Dreanno, Catherine; Fauchot, Juliette; Fifas, Spyros; Guyader, Olivier; Jean, Frederic; Le Gallic, Bertrand; Riou, Philippe; Schapira, Mathilde; Thiebaut, Eric.
The King scallop Pecten maximus constitutes the first landed species in terms of tonnage and the second or third one in terms of value for the French fisheries. More than 90% of these landings come from the English Channel indicating that its exploitation is essential to local fleets. The COMANCHE project (Ecosystem Interactions and anthropogenic impacts on King scallops populations in the English Channel) proposed to improve the knowledge of the scallop within the Channel, through an ecosystem-based approach for fisheries, appealing to a wide range of scientific disciplines (physics, chemistry, genetics, ecology, geostatistics, modeling, economics ....). Researches on the spatial location of scallop beds, connectivity by larval dispersal between beds,...
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00251/36236/34797.pdf
Imagem não selecionada

Imprime registro no formato completo
Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa ArchiMer
Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland.
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use...
Tipo: Text Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf
Imagem não selecionada

Imprime registro no formato completo
Marine fish spermatozoa: racing ephemeral swimmers ArchiMer
Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R.
After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply....
Tipo: Text Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf
Imagem não selecionada

Imprime registro no formato completo
New Approach For The Detection Of Toxic Algae Species ArchiMer
Colas, Florent; Crassous, Marie-pierre; Laurent, Sebastien; Dreanno, Catherine; Compere, Chantal.
Up to now, toxic species are monitored by expensive and time-consuming laboratory analysis of seawater samples: after a 24h decantation, the samples are observed with an optical microscope and the cell concentration per species is determined. In this context, systems able to detect HAB species directly underwater would be of great help. An assay based on an SPR biosensor was then developed for detecting Alexandrium minutum.
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00089/19995/17658.pdf
Imagem não selecionada

Imprime registro no formato completo
NOUBA Project Novel Natural Molecules Inhibiting the Development of Marine Bacteria in Biofilms ArchiMer
Klein, Geraldine; Soum-soutera, Emmanuelle; Bazire, Alexis; Dreanno, Catherine; Compere, Chantal; Dufour, Alain.
Marine organisms represent a rather unexplored source of new activity and biological functions of molecules for biotechnologies. The research in chemical ecology of the marine environment leads to looking at metabolites of recognition or defence produced by these models. Marine bacteria belonging to the Pseudoalteromonas genus of the Gammaproteobacteria class are often found in association with marine eukaryotes, and their ability to produce a variety of biological activities attracted a particular attention. The marine Pseudoalteromonas sp. 3J6 and D41 were selected for their capacity to inhibit the biofilm formation of other bacteria. The study of antibiofilm metabolites synthesised by marine bacteria 3J6 and D41 biofilms can lead to the development of...
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00089/19990/17666.pdf
Imagem não selecionada

Imprime registro no formato completo
Phytochip: Development of a DNA-microarray for rapid and accurate identification of Pseudo-nitzschia spp and other harmful algal species ArchiMer
Noyer, Charlotte; Abot, Anne; Trouilh, Lidwine; Leberre, Veronique Anton; Dreanno, Catherine.
Detection of harmful algal blooms has become a challenging concern because of to the direct impacts on public health and economy. The identification of toxic dinoflagellates and diatoms in monitoring programs requires an extensive taxonomic expertise and is time consuming. Advances in molecular biology have allowed the development of new approaches, more rapid, accurate and cost-effective for detecting these microorganisms. In this context, we developed a new DNA microarray (called, Phytochip) for the simultaneous detection of multiple HAB species with a particular emphasis on Pseudo-nitzschia species. Oligonucleotide probes were designed along the rRNA operon. After DNA extraction, the target rDNA genes were amplified and labeled using an asymmetric PCR;...
Tipo: Text Palavras-chave: HAB; Pseudo-nitzschia; Microarray; Ribosomal oligonucleotide probes; Environmental monitoring.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36554/42346.pdf
Imagem não selecionada

Imprime registro no formato completo
Rapid detection and quantification of the marine toxic algae, Alexandrium minutum, using a super-paramagnetic immunochromatographic strip test ArchiMer
Gas, Fabienne; Baus, Beatrice; Quere, Julien; Chapelle, Annie; Dreanno, Catherine.
The dinoflagellates of Alexandrium genus are known to be producers of paralytic shellfish toxins that regularly impact the shellfish aquaculture industry and fisheries. Accurate detection of Alexandrium including A. minutum is crucial for environmental monitoring and sanitary issues. In this study, we firstly developed a quantitative lateral flow immunoassay (LFIA) using super-paramagnetic nanobeads for A. minutum whole cells. This dipstick assay relies on two distinct monoclonal antibodies used in a sandwich format and directed against surface antigens of this organism. No sample preparation is required. Either frozen or live cells can be detected and quantified. The specificity and sensitivity are assessed by using phytoplankton culture and field samples...
Tipo: Text Palavras-chave: Alexandrium minutum; Detection; Quantification; Dipstick lateral flow immunoassay; Super-paramagnetic.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00286/39695/38152.pdf
Imagem não selecionada

Imprime registro no formato completo
Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm ArchiMer
Chereguini, Olvido; Cal, Rosa Maria; Dreanno, Catherine; Ogier De Baulny, Bénédicte; Suquet, Marc; Maisse, Gérard.
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 degrees C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
Tipo: Text Palavras-chave: Turbot; Sperm; Storage; Cryopreservation.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00176/28724/27173.pdf
Imagem não selecionada

Imprime registro no formato completo
Toward in situ detection of algae species ArchiMer
Colas, Florent; Laurent, Sebastien; Lehaitre, Michel; Hamelin, Muriel; Crassous, Marie-pierre; Antoine, Elisabeth; Dreanno, Catherine; Deuff, Albert; Compere, Chantal.
For the last few years there is a more and more pressing need to develop system for detecting HAB at their early stage. Researchers have then been working on new in situ sensor. In this paper a submarine transducer based on surface plasmon resonance is reported. It demonstrated a refractive index resolution of 3.10(-6), which is promising for later phytoplankton detection.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00107/21802/20328.pdf
Imagem não selecionada

Imprime registro no formato completo
Tracking, understanding and predictingtoxic phytoplankton blooms and their effects scallops populations in the bay of Seine: the comanche projet ArchiMer
Schapira, Mathilde; Le Gendre, Romain; Fiant, Liliane; Fauchot, Juliette; Raimbault, René; Dreanno, Catherine; Claquin, Pascal; Riou, Philippe.
Tipo: Text
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00056/16721/14175.pdf
Registros recuperados: 17
Primeira ... 1 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional