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Accuracy of BLUP breeding values in a factorial mating design with mixed families and marker-based parentage assignment in rainbow trout Oncorhynchus mykiss ArchiMer
Haffray, Pierrick; Enez, Florian; Bugeon, Jerome; Chapuis, Herve; Dupont-nivet, Mathilde; Chatain, Beatrice; Vandeputte, Marc.
Marker-based parentage assignment provides the opportunity to investigate factors of efficiency for mixed-family designs and factorial mating. In such designs, family size is both uncontrolled and small, which may be thought to limit the accuracy of estimated breeding values (EBVs). The objective of this work was to estimate the accuracy of EBVs of growth and quality traits in a large factorial mating design and in commercial breeding conditions. An expected six hundred full-sib families of rainbow trout Oncorhynchus mykiss (2042 fish in total) were produced by ten factorial matings of six dams with ten sires. Fish were phenotyped for body weight, carcass yield, fillet yield, fillet fat content and fillet colour, and family information was recovered using...
Tipo: Text Palavras-chave: Aquaculture; Salmonids; DNA parentage assignment; Sib selection; EBV accuracy.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54062/55395.pdf
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Procédure de qualification des donnnées Quadrige² - Données chimie RNOPHY (Imposex) ArchiMer
Enez, Florian; Gauthier, Emilie; Grouhel-pellouin, Anne.
La qualification a pour objectif d’attribuer un niveau de qualité aux données : Bon, Douteux ou Faux. Le processus de qualification consiste à rechercher des anomalies plus ou moins complexes dans les données stockées dans la base de données Quadrige², les anomalies étant définies par les responsables de programme Quadrige ou responsables thématiques. Ces recherches d’anomalies sont faites en lien avec la cellule quadrige². La qualification des données peut se faire : • de façon ponctuelle : identification d’un problème de processus analytique pour quelques données • en routine : un processus de qualification dite « automatique » permet de qualifier les enregistrements individuellement (cohérence des informations de chaque résultat individuellement). Puis...
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Ano: 2018 URL: http://archimer.ifremer.fr/doc/00455/56632/58367.pdf
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Transcriptome based SNP discovery and validation for parentage assignment in hatchery progeny of the European abalone Haliotis tuberculata ArchiMer
Harney, Ewan; Lachambre, Sebastien; Roussel, Sabine; Huchette, Sylvain; Enez, Florian; Morvezen, Romain; Haffray, Pierrick; Boudry, Pierre.
Selective breeding strategies require pedigree information over generations, but many species produced in aquaculture are too small to be physically tagged at early stages. Consequently, maintaining a sufficient number of separate families is often needed but costly and logistically difficult. Alternatively, parentage assignment can be obtained using DNA markers. We developed a panel of single nucleotide polymorphism (SNP) markers for the European abalone Haliotis tuberculata using an existing transcriptomic resource. An initial set of 2,176,887 SNPs was filtered to select 500 for high throughput genotyping. Of these, 298 SNPs were amplified in at least 90% of our H. tuberculata samples, consisting of a mixed family cohort (945 offspring) generated by...
Tipo: Text Palavras-chave: Abalone; Parentage assignment; Pedigree; Selective breeding; Transcriptome; SNP.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00429/54061/55396.pdf
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