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Analysis of genetic diversity in the Oryza officinalis complex Electron. J. Biotechnol.
Federici,María Teresa; Shcherban,Andrei B.; Capdevielle,Fabián; Francis,Marta; Vaughan,Duncan.
The genetic relationships among 34 accessions of wild rice from Asia, Africa, America and Australia were analysed using RFLP technique. After southern blotting, DNA digestion pattern was hybridised with a highly repetitive DNA sequence of a retrotransposon from a gypsy family of mobile elements. A dendrogram was constructed from RFLP data in which the species clustered according to their genome designation (CC, BB, BBCC and CCDD genomes). Some species did not appear in the same group, for example, O. eichingeri from Africa and Sri Lanka clustered separately from each other. The same situation was observed for the accessions from China of O. officinalis, which cluster together showing a close relationship with O. rhizomatis, and O. eichingeri (both of CC...
Tipo: Journal article
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000200012
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Analysis of Uruguayan weedy rice genetic diversity using AFLP molecular markers Electron. J. Biotechnol.
Federici,María Teresa; Vaughan,Duncan; Norihiko,Tomooka; Kaga,Akita; Xin,Wang Wang; Koji,Doi; Francis,Marta; Zorrilla,Gonzalo; Saldain,Nestor.
Weedy rice is a serious problem in Uruguayan rice fields since intensification of rice production started about 10 years ago. The genetic diversity of 26 weedy accessions of weedy rice and 6 Uruguayan cultivars were analyzed using AFLP (amplified fragment length polymorphisms) methodology. Abundant polymorphisms were found among samples tested. Using different methods of analysis three groups of samples were revealed. A relationship was found between three groups and morphological traits. One group had a black hull, purple apex and long awn (wild type traits) while another group had straw hull and apex, and short or no awn (domestication traits). The third group included the cultivars analyzed and some weedy rice samples. The weedy rice in this third group...
Tipo: Journal article
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582001000300008
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Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis Electron. J. Biotechnol.
Federici,María Teresa; Marcondes,Jackson A; Picchi,Simone Cristina; Stuchi,Eduardo S; Fadel,André Luiz; Laia,Marcelo L; Lemos,Manoel Victor F; Lemos,Eliana Gertrudes Macedo.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding...
Tipo: Journal article Palavras-chave: Citrus; Microarray; Xylella fastidiosa.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000300004
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