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Tolerance and stress response of Macrolepiota procera to nickel IPB - Escola Superior Agrária
Baptista, Paula; Ferreira, Sílvia; Soares, Maria Elisa; Coelho, Valentim; Bastos, Maria de Lourdes.
Nickel (Ni) is an essential element for many organisms; however, it is very toxic at high concentrations and also depending on the species. In macrofungi the mechanisms underlying their Ni tolerance are poorly documented. This study examines, for the first time, the participation of the antioxidative system in Macrolepiota procera exposed to different Ni2+ concentrations and their relation with Ni tolerance. The effect of the pH on Ni tolerance was also evaluated. The fungus was cultivated on solid medium with different NiCl2 concentrations (0.05, 0.2, 0.8 mM) at pH 4, 6, and 8, and fungi growth and Ni uptake were determined. The antioxidative enzymes catalase (CAT) and superoxide dismutase (SOD) and the production of hydrogen peroxide (H2O2) were...
Tipo: Article Palavras-chave: Macrolepiota procera; Nickel; Stress; Ni2+ tolerance; Ni2+ accumulation; Hydrogen peroxide; Antioxidant enzymes.
Ano: 2009 URL: http://hdl.handle.net/10198/4473
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Tolerance and stress response of the saprobe macrofungi Macrolepiota procera to nickel IPB - Escola Superior Agrária
Baptista, Paula; Ferreira, Sílvia; Soares, Maria Elisa; Bastos, Maria de Lourdes.
Nickel (Ni) is an essential element for many organisms however it is very toxic at high concentrations and also depending on the species. In macrofungi the mechanisms underlying their Ni-tolerance are poorly documented. We examined, for the first time, the participation of the antioxidative system in the Macrolepiota procera exposed to different Ni2+ concentrations and their relation with Ni-tolerance. The effect of the pH on Ni-tolerance was also evaluated. The fungus was cultivated on solid medium with different Ni concentrations (0.05, 0.2, 0.8 mM) at pH 4, 6 and 8, and fungi growth and Ni uptake was determined. The antioxidative enzymes, catalase (CAT) and superoxide dismutase (SOD) and the production of hydrogen peroxide (H2O2) were evaluated on...
Tipo: ConferenceObject Palavras-chave: Macrolepiota procera; Nickel; Stress; Ni-tolerance; Ni-accumulation; Hydrogen peroxide; Antioxidant enzymes.
Ano: 2009 URL: http://hdl.handle.net/10198/4488
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