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Registros recuperados: 28
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A PRELIMINARY STUDY FOR ELIMINATION OF CONTAMINATED BABESIA MICROTI FROM THE MODEL OF HUMAN PLASMA FRACTION OAK
Nishisaka, M.; Igarashi, Ikuo; Fujisaki, Kozo; Miyahara, T.; Ogata, Y.; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Babesia microti; Human plasma fraction; Filtration.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/629
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A Review of the Taxonomy of Theileria sergenti/buffeli/orientalis Group Parasites in Cattle OAK
Fujisaki, Kozo.
The controversial circumstances relating to the classification of the benign Theileria species from Japan, Australia and Britain, which are frequently referred to as T. sergenti/buffeli/orientalis group parasites, was reviewed. Our recent systematic comparisons suggest a possibility that the Japanese T. sergenti (minor piroplasm in common parlance) might be a new species and the Australian T. buffeli and British T. orientalis might belong to one and the same species. The necessity for the review of the genus name for these benign Theileria species was also briefly discussed after the morphological studies of their schizogony.
Palavras-chave: Theileria sergenti/buffeli/orientalis; Taxonomy; Cattle.
Ano: 1992 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/168
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A trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks in Kagoshima Prefecture, Japan. OAK
Thekisoe, Oriel M. M.; Honda, T; Fujita, H; Battsetseg, B; Hatta, T; Fujisaki, Kozo; Sugimoto, Chihiro; Inoue, Noboru.
Common arthropod vectors for trypanosomes are flies, fleas and bugs. This study reports on an unknown trypanosome species isolated from naturally infected Haemaphysalis hystricis ticks, hereby, referred to as Trypanosoma KG1 isolate. The parasite has been successfully cultured in vitro with L929 or HEK 293T cell line as feeder cells. This trypanosome cannot survive in vitro without feeder cells. Following experimental infections of ticks, the trypomastigote-like and the epimastigote-like forms of this trypanosome could be detected by Giemsa-stained smears of the midgut and salivary glands of Ornithodoros moubata ticks which were made to feed on a culturing medium containing Trypanosoma KG1 isolate through an artificial membrane. Trypanosoma KG1 isolate...
Palavras-chave: Trypanosoma KG1 isolate; Haemaphysalis hystricis; Ornithodoros moubata.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1047
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Advances in the artificial feeding of Ornithodoros moubata (Acari: Ixodidae) and the follow up of its life cycle after feeding on fetal bovine serum OAK
Ruheta, Martin R.; Inoue, Noboru; Fujisaki, Kozo.
Ornithodoros moubata was fed artificially through a parafilm membrane with two types of artificial meals. The first type was composed of bovine and horse red blood cells suspended in phosphate buffered saline or fetal bovine serum (FBS). The second type was composed of FBS alone. Engorgement and post engorgement survival rates of the ticks after feeding on the two types of meals were compared and analysed statistically. FBS was found to be superior to red blood cell meals. Eventually two batches of FBS from different manufacturers were used to feed the ticks through artificial membrane. Engorgement and post engorgement survival rates were again compared and analysed statistically. There was no significant difference between the two lots. Finally O. moubata...
Palavras-chave: Ornithodoros moubata; Fetal bovine serum; Bovine red blood cells; Horse red blood cells; Artificial membrane feeding.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/152
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Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites OAK
Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034
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Babesia gibsoni: An apical membrane antigen-1 homologue and its antibody response in the infected dogs OAK
Zhou, Jinlin; Yang, Jun; Zhang, Guohong; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the apical membrane antigen-1 (AMA-1) homologue was obtained by immunoscreening a cDNA expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 2062 bp. Computer analysis suggested that the sequence contains an open reading frame of 1794 bp with a coding capacity of approximately 66 kDa. Based on the homology analysis, this putative protein was designated as B. gibsoni AMA-1 (BgAMA-1). The BgAMA-1 gene was expressed in the Escherichia coli BL21 strain and used as the antigen in Western blotting and the enzyme-linked immunosorbent assay (ELISA). The results indicated that BgAMA-1 was recognized as an immunodominant antigen by the host immune system and that it induced a strong antibody...
Palavras-chave: Babesia gibsoni; AMA-1; Antibody response.
Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/815
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Babesia gibsoni rhoptry-associated protein 1 and its potential use as a diagnostic antigen OAK
Zhou, Jinlin; Jia, Honglin; Nishikawa, Yoshifumi; Fujisaki, Kozo; Xuan, Xuenan.
A cDNA encoding the rhoptry-associated protein 1 (RAP-1) homologue was obtained by immunoscreening an expression library prepared from Babesia gibsoni merozoite mRNA. The complete nucleotide sequence of the gene was 1740bp. Computer analysis suggested that the sequence contains an open reading frame of 1425bp encoding an expected protein with a molecular weight of 52kDa. Based on the sequence similarity, this putative protein was designated as the B. gibsoni RAP-1 (BgRAP-1). The BgRAP-1 gene was expressed in the Escherichia coli BL21 strain, and the recombinant BgRAP-1 was used as the antigen in the enzyme-linked immunosorbent assay (ELISA). The results can differentiate between the B. gibsoni-infected dog sera and the Babesia canis infected dog sera or...
Palavras-chave: Babesia gibsoni; Rhoptry-associated protein; Enzyme-linked immunosorbent assay; Diagnosis.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/811
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Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B-microti infection in mice OAK
Terkawi, M. Alaa; Jia, Honglin; Zhou, Jinlin; Lee, Eung-goo; Igarashi, Ikuo; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan.
Babesia gibsoni ribosomal phosphoprotein PO (BgP0) was identified as an immunodominant cross-reactive antigen with B. microti. The BgPO gene is a single copy with a predicted open reading frame of 942 bp and 314 amino acids. The BgP0 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice with the recombinant BgPO showed a specific band with a 34-kDa molecular mass in the extracts of B. gibsoni and B. microti merozoites. Furthermore, the intraperitoneal (i.p.) immunization of rBgP0 and Freund's adjuvant induced strong Immoral response consisting of mixed immunoglobulins IgG1 and IgG2a in BALB/c mice. Following the challenge with B. microti, these mice delayed the onset of parasites and significantly reduced...
Palavras-chave: B. gibsoni; B. microti; Ribosomal phosphoprotein P0; Cross-reactive antigen.
Ano: 2007 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1036
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Cellular localization of Babesia bovis rhoptry-associated protein 1 in the merozoite stage with immuno-electron microscopy OAK
Matsuo, Tomohide; Yokoyama, Naoaki; Suthisak, Boonchit; Fujisaki, Kozo; Igarashi, Ikuo.
We examined the detailed cellular localization of the rhoptry-associated protein-1 (RAP-1) of Babesia bovis merozoites by the post-embedding method of immuno-electron microscopy (IEM) using the anti-RAP-1 monoclonal antibody (mAb) 1C1 in order to substantiate the result in our previous report by an indirect immunofluorescent antibody test and the pre-embedding method of IEM. RAP-1 slightly distributed only in cytoplasm of merozoites, especially around the nucleus, but not in the rhoptry organelle at an early stage after invasion into a red blood cell. Then, the RAP-1 was accumulated in the rhoptry organelle and was also found in the iRBC cytoplasm, which suggests that synthesis and release of RAP-1 may occur in the iRBC. Finally, the RAP-1 was found within...
Palavras-chave: Babesia bovis RAP-1 invasion; Escape RBC.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/145
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CHARACTERIZATION OF CD11c+ DENDRITIC CELL POPULATIONS IN SPLEENS OF MICE INFECTED WITH TOXOPLASMA GONDII OAK
Makala, Levi H. C; Zayatiin, Batsukh; Kamada, Takenori; Seng, Seyha; Ribas, Lazaro; Mishima, Masayuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Nagasawa, Hideyuki.
Palavras-chave: Spleen; Dendritic cell; Mouse; Toxoplasma lysate antigen (TLA).
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/625
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Characterization of P15 Antigen of Cryptosporidium parvum Expressed by a Recombinant Vaccinia Virus OAK
Xuan, Xuenan; Zhang, Sofa; Kamio, Tsugihiko; Tsushima, Y.; Kamada, Takenori; Nishikawa, Yoshifumi; Otsuka, Haruki; Karanis, Panagiotis; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; 玄, 学南; 西川, 義文; 五十嵐, 郁男.
Palavras-chave: C. parvum; P15; Vaccinia virus; Subunit vaccine.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/314
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CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS OAK
Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624
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Construction of Recombinant Feline Herpesvirus Type 1 Expressing Toxoplasma gondii SRS1 OAK
Mishima, Masayuki; Xuan, Xuenan; Takeiri, Akira; Makala, Levi H. C; Igarashi, Ikuo; Fujisaki, Kozo; Nagasawa, Hideyuki; Mikami, Takeshi.
Palavras-chave: Toxoplasma; Vaccine; SRS1; FHV.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/343
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Construction of the recombinant pseudorabies viruses expressing Cryptosporidium parvum an immunodominant surface protein, p23 OAK
Takashima, Yasuhiro; Xuan, Xuenan; Nagasawa, Hideyuki; Matsumoto, Yasunobu; Igarashi, Ikuo; Fujisaki, Kozo; Mikami, Takeshi; Otsuka, Haruki; 玄, 学南; 五十嵐, 郁男.
To develop a vaccine against cryptosporidiosis in animals, we constructed recombinant pseudorabies virus (PrV), a member of the Herpesviridae Alphaherpesvirus subfamily, expressing an immunodominant surface protein p23 of Cryptosporidium parvum sporozoites. Because of the wide host range of PrV, it has the possibility as the vector to delivery the foreign genes to several species of animals containing experiment animal. In the recombinant constructed in this study, the p23 gene under the control of CAG promoter was integrated into the thymidine kinase (TK) gene of PRV. Antibody against p23 recognized p23 expressed in CPK cells infected with the recombinant, as the approximate 23 kDa specific band in Western blotting analysis. This study showed the...
Palavras-chave: Cryptosporidium parvum; P23; Herpes; Pseudorabies virus; Subunit vaccine.
Ano: 2000 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/129
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Detection of Antibodies to Babesia equi by The Latex Agglutination Test with Recombinant Merozoite Antigen-2 OAK
Tanaka, Tetsuya; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男.
Palavras-chave: Babesia equi; EMA-2; Baculovirus; Latex agglutination test.
Ano: 1999 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/329
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Development of DNA Probes for the Detection and Identification of Babesia ovata OAK
Kawazu, Shin-ichiro; Panchadcharam, Chandrawathani; Kawazu, Takeshi; Sekizaki, Tsutomu; Fujisaki, Kozo.
Palavras-chave: DNA probe diagnosis; Babesia ovata.
Ano: 1993 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/187
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Diagnosis of Babesia caballi Infection in Horses by Polymerase Chain Reaction OAK
Xuan, Xuenan; Igarashi, Ikuo; Avarzed, A.; Ikadai, Hiromi; Inoue, Noboru; Nagasawa, Hideyuki; Fujisaki, Kozo; Toyoda, Yutaka; Suzuki, Naoyoshi; Mikami, Takeshi; 玄, 学南; 五十嵐, 郁男; 井上, 昇.
A set of primers were designed according to the published sequence of the gene encoding a rhoptry protein of Babesia caballi, and used to amplify parasite DNA from the blood samples obtained from carrier horses by polymerase chain reaction(PCR)method.The PCR method was sensitive enough to detect parasite DNA from 2.5 μl blood sample with a parasitemia of 0.000001%. The PCR method was compared with fluorescent antibody test(IFAT) in order to evaluate the diagnosis effciency for B. caball infection in horses. Of 142 field samples from Mongolia, 28(20%) and 96(69%)samples were identified positively by PCR and IFAT, respectively. Although the sensitivity of PCR was lower than IFAT, it was noted that the 5 IFAT-negative samples were PCR-positive, suggesting...
Palavras-chave: Babesia caballi; PCR; IFAT.
Ano: 1998 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/281
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DNA Probes for Detection of Babesia ovata or the Babesia sp.1, A Newly Isolated Bovine Babesia Parasite in Japan OAK
Ohta, Masato; Kawazu, Shin-ichiro; Tsuji, Naotoshi; Terada, Yutaka; Kamio, Tsugihiko; Fujisaki, Kozo.
Palavras-chave: Babesia ovata; DNA probe.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/226
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Emergence of multi-acaricide resistant Rhipicephalus ticks and its implication on chemical tick control in Uganda OAK
Vudriko, Patric; Okwee-Acai, James; Tayebwa, Dickson Stuart; Byaruhanga, Joseph; Kakooza, Steven; Wampande, Edward; Omara, Robert; Muhindo, Jeanne Bukeka; Tweyongyere, Robert; Owiny, David Okello; Hatta, Takeshi; Tsuji, Naotoshi; Umemiya-Shirafuji, Rika; Xuan, Xuenan; Kanameda, Masaharu; Fujisaki, Kozo; Suzuki, Hiroshi.
Background: Acaricide failure has been on the rise in the western and central cattle corridor of Uganda. In this study, we identified the tick species associated with acaricide failure and determined their susceptibility to various acaricide molecules used for tick control in Uganda. Methods: In this cross sectional study, tick samples were collected and identified to species level from 54 purposively selected farms (from 17 districts) that mostly had a history of acaricide failure. Larval packet test was used to screen 31 tick populations from 30 farms for susceptibility at discriminating dose (DD) and 2 x DD of five panels of commercial acaricide molecules belonging to the following classes; amidine, synthetic pyrethroid (SP), organophosphate (OP) and...
Palavras-chave: Ticks; Rhipicephalus appendiculatus; Rhipicephalus (Boophilus) decoloratus; Acaricide; Resistance; Amitraz; Synthetic pyrethroids; Organophosphates.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4437
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Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii OAK
Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.
Ano: 2005 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816
Registros recuperados: 28
Primeira ... 12 ... Última
 

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