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ITS-rDNA phylogeny of Colletotrichum spp. causal agent of apple Glomerella leaf spot Ciência Rural
Giaretta,Diorvania Ribeiro; Bogo,Amauri; Coelho,Cileide Maria Medeiros; Guidolin,Altamir Frederico; Dantas,Adriana Cibele de Mesquita; Gomes,Eliane Aparecida.
Several diseases have affected apple production, among them there is Glomerella leaf spot (GLS) caused by Colletotrichum spp. The first report of this disease in apple was in plants nearby citrus orchards in São Paulo State, Brazil. The origin of this disease is still not clear, and studies based on the molecular phylogeny could relate the organisms evolutionarily and characterize possible mechanisms of divergent evolution. The amplification of 5.8S-ITS (Internal Transcribed Spacer) of rDNA of 51 pathogenic Colletotrichum spp. isolates from apples, pineapple guava and citrus produced one fragment of approximately 600 bases pairs (bp) for all the isolates analyzed. The amplified fragments were cleaved with restriction enzymes, and fragments from 90 to 500bp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pineapple guava; Citrus; Molecular phylogeny; In silico; Phylogenetic tree.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400010
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