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Haemolymph electrophoretic pattern of Ascia monuste orseis larvae (Lepidoptera: pieridae) parasitized by Cotesia glomerata (Hymenoptera: braconidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Scaglia,M.; Brochetto-Braga,M. R.; Chaud-Netto,J.; Gobbi,N..
Cotesia glomerata is a natural enemy of the vegetable plague Ascia monuste orseis and preferably parasites 2nd, 3rd and 4th instar larvae. Parasitism effects on the haemolymph protein profile of Ascia monuste orseis larvae from the 2nd to 7th days were analyzed both qualitatively and quantitatively by SDS-PAGE and Coomassie-Blue binding methods. Quantitative analysis showed a progressive increase in the protein content of about 6.5 and 12.5 times in parasitized and non-parasitized larvae from the 2nd to 5th days, respectively. On the 6th day, a decrease in protein content was observed in both groups, although this decrease was significantly less than the control group that continued to metamorphosis. Meanwhile, parasitized larvae had one more day (7th day)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascia monuste orseis; Cotesia glomerata; Electrophoretic protein profile; Haemolymph; Natural enemy; Parasitism; SDS-PAGE.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000100006
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Insects as biological models to assay spider and scorpion venom toxicity J. Venom. Anim. Toxins incl. Trop. Dis.
Manzoli-Palma,M. F.; Gobbi,N.; Palma,M. S..
This study was undertaken to develop an experimental protocol using insects as biological models to assay venom toxicity of the following spiders Loxosceles gaucho, Phoneutria nigriventer, Nephilengys cruentata and Tityus serrulatus scorpion. Three different insect species were bioassayed: Apis mellifera (Hymenoptera), Grillus assimilis (Orthoptera), and Diatraea saccharalis (Lepidoptera). Venoms were injected into the hemocele of insects with a microsyringe at concentrations that caused dose/weight-dependent effects; doses causing either paralysis (ED50) or death (LD50) were recorded for each venom and insect test-species. T. serrulatus and L. gaucho venoms were lethal to all tested species, while P. nigriventer venom caused paralysis and death, and N....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioassay; Venom; Insect model; Toxicity; Apis mellifera.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992003000200004
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