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Registros recuperados: 8
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A method for drought tolerance screening in sunflower Inra
Pereyra-Irujo, G.A.; Velazquez, L.; Granier, C.; Aguirrezabal, L.A.N..
One difficulty in obtaining drought-tolerant varieties is the unequivocal evaluation of plant response to soil-water deficits. A method is proposed that allows reproducible soil-water deficit treatments to be applied to different sunflower genotypes. Maize (Zea mays L.) plants were sown in the same pots as but earlier than some sunflower plants in order to rapidly deplete the soil water. This method was compared to a standard method in which plants under evaluation are used to dry out the soil. The method was reproducible in the evaluation of drought tolerance of four sunflower inbred lines. Advantages of the proposed method are that: (i) it allows the desired soil water content to be reached quickly; (ii) the resulting soil water content in the pot is...
Tipo: Journal Article Palavras-chave: DROUGHT TOLERANCE; WATER DEFICIT; LEAF AREA; WATER DEPLETION RATE; SOIL WATER DISTRIBUTION.
Ano: 2007 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20088e4335dc&uri=/notices/prodinra1/2008/09/
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Co-ordination of cell division and tissue expansion in sunflower, tobacco, and pea leaves: dependence or independence of both processes ? Inra
Granier, C.; Turc, O.; Tardieu, F..
Temporal analyses of cell division and tissue expansion in pea, tobacco, and sunflower leaves reveal that both processes follow similar patterns during leaf development. Relative cell division and relative tissue expansion rates are maximal and constant during early leaf development, but they decline later. In contrast, relative cell expansion rate follows a bell-shaped curve during leaf growth. Cell division and tissue expansion have common responses to temperature, intercepted radiation, and water deficit. As a consequence, final leaf area and cell number remain highly correlated throughout a large range of environmental conditions for these different plant species, indicating that cell division and tissue expansion are co-ordinated during leaf...
Tipo: Journal Article Palavras-chave: CROISSANCE; DEVELOPPEMENT DES FEUILLES; EXPANSION DES TISSUS; ANALYSE CINEMATIQUE LEAF DEVELOPMENT; CELL DIVISION; TISSUE EXPANSION; DICOTYLEDONOUS; KINEMATIC ANALYSES.
Ano: 2000 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0200006642092864&uri=/notices/prodinra1/2010/12/
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Développement d’un système d'information de phénotypage d’Arabidopsis thaliana Inra
Fabre, J.; Tireau, A.; Neveu, P.; Dauzat, M.; Granier, C..
L'évolution de la démarche scientifique dans la plupart des champs d'investigation se traduit par une exigence de haut débit dont on ne peut tirer le meilleur profit sans un effort considérable d’archivage et de mise à disposition des données. Au laboratoire d’écophysiologie des plantes sous stress environnementaux (LEPSE) du centre Inra de Montpellier, le développement récent d’une plate-forme de phénotypage automatisée dédiée à la plante modèle Arabidopsis thaliana, la plate-forme PHENOPSIS, a permis d’augmenter de façon considérable les analyses phénotypiques effectuées sur cette espèce. Dans ce contexte, il est devenu nécessaire de développer une base de données associée à la plate-forme. Le travail présenté ici décrit les choix technologiques et le...
Tipo: Journal Article Palavras-chave: SYSTEME D'INFORMATION; SYSTEME DE GESTION DE BASE DE DONNEES; INTERFACE WEB; PHENOTYPAGE; HAUT-DEBIT; ARABIDOPSIS THALIANA; MYSQL; PHP; HTML; ARCHITECTURE CLIENT/SERVEUR.
Ano: 2008 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD20103ace6cad&uri=/notices/prodinra1/2010/12/
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Genetic analysis identifies quantitative trait loci controlling rosette mineral concentrations in Arabidopsis thaliana under drought Inra
Ghandilyan, A.; Barboza, L.; Tisne, S.; Granier, C.; Reymond, M.; Koornneef, M.; Schat, H.; Aarts, M.G.M..
Rosettes of 25 Arabidopsis thaliana accessions and an Antwerp-1 (An-1) x Landsberg erecta (Ler) population of recombinant inbred lines (RILs) grown in optimal watering conditions (OWC) and water deficit conditions (WDC) were analysed for mineral concentrations to identify genetic loci involved in adaptation of mineral homeostasis to drought stress.Correlations between mineral concentrations were determined for accessions and a quantitative trait locus (QTL) analysis was performed for the RIL population.Plant growth and rosette mineral contents strongly decreased in WDC compared with OWC. Mineral concentrations also generally decreased, except for phosphorus (P), which remained constant, and potassium (K), which increased. Large variations in mineral...
Tipo: Journal Article Palavras-chave: ARABIDOPSIS THALIANA ACCESSIONS; MINERAL CONCENTRATION; QUANTITATIVE TRAIT LOCUS ANALYSIS; WATER STRESS.
Ano: 2009 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010d525e3a3&uri=/notices/prodinra1/2010/08/
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High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll Inra
Wuyts, N.; Palauqui, J.C.; Conejero, G.; Verdeil, J.L.; Granier, C.; Massonnet, C..
BackgroundDespite the wide spread application of confocal and multiphoton laser scanning microscopy in plant biology, leaf phenotype assessment still relies on two-dimensional imaging with a limited appreciation of the cells' structural context and an inherent inaccuracy of cell measurements. Here, a successful procedure for the three-dimensional imaging and analysis of plant leaves is presented.ResultsThe procedure was developed based on a range of developmental stages, from leaf initiation to senescence, of soil-grown Arabidopsis thaliana (L.) Heynh. Rigorous clearing of tissues, made possible by enhanced leaf permeability to clearing agents, allowed the optical sectioning of the entire leaf thickness by both confocal and multiphoton microscopy. The...
Tipo: Journal Article Palavras-chave: IMAGERIE TRI DIMENSIONNELLE; MICROSCOPIE MULTIPHOTONIQUE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010fefa07c5&uri=/notices/prodinra1/2010/08/
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Mapping the paratope of anti-CD4 recombinant fab 13B8.2 by combining parallel peptide synthesis and site-directed mutagenesis Inra
Bès, C.; Briand-Longuet, L.; Cerutti, M.; Heitz, F.; Troadec, S.; Pugnière, M.; Roquet, F.; Molina, F.; Casset, F.; Bresson, D.; Péraldi-Roux, S.; Devauchelle, G.; Devaux, C.; Granier, C.; Chardès, T..
We analyzed antigen-binding residues from the variable domains of anti-CD4 antibody 13B8.2 using the Spot method of parallel peptide synthesis. Sixteen amino acids, defined as Spot critical residues (SCR), were identified on the basis of a 50% decrease in CD4 binding to alanine analogs of reactive peptides. Recombinant Fab 13B8.2 mutants were constructed with alanine residues in place of each of the 16 SCR, expressed in the baculovirus cell system, and purified. CD measurements indicated that the mutated proteins were conformationally intact, with a β-sheet secondary structure similar to that of wild-type Fab. Compared with the CD4-binding capacity of wild-type Fab 13B8.2, 11 light (Y32-L, W35-L, Y36-L, H91-L, and Y92-L) and heavy chain (H35-H, R38-H,...
Tipo: Journal Article Palavras-chave: ANTICORPSMUTATION; CARACTERISATION.
Ano: 2003 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025195106087&uri=/notices/prodinra1/2010/11/
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Probing the reproducibility of leaf growth and molecular phenotypes: A comparison of three Arabidopsis accessions cultivated in ten laboratories Inra
Massonnet, C.; Vile, D.; Fabre, J.; Hannah, M.A.; Caldana, C.; Lisec, J.; Beemster, G.T.S.; Meyer, R.C.; Messerli, G.; Gronlund, J.T.; Perkovic, J.; Wigmore, E.; May, S.; Bevan, M.W.; Meyer, C.; Rubio-Diaz, S.; Weigel, D.; Micol, J.L.; Buchanan-Wollaston, V.; Fiorani, F.; Walsh, S.; Rinn, B.; Gruissem, W.; Hilson, P.; Hennig, L.; Willmitzer, L.; Granier, C..
A major goal of the life sciences is to understand how molecular processes control phenotypes. Because understanding biological systems relies on the work of multiple laboratories, biologists implicitly assume that organisms with the same genotype will display similar phenotypes when grown in comparable conditions. We investigated to what extent this holds true for leaf growth variables and metabolite and transcriptome profiles of three Arabidopsis (Arabidopsis thaliana) genotypes grown in 10 laboratories using a standardized and detailed protocol. A core group of four laboratories generated similar leaf growth phenotypes, demonstrating that standardization is possible. But some laboratories presented significant differences in some leaf growth variables,...
Tipo: Journal Article Palavras-chave: PHENOTYPE; RELATION GENOTYPE x ENVIRONNEMENT; SIGNATURE METABOLIQUE.
Ano: 2010 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2010a046194&uri=/notices/prodinra1/2010/08/
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Synthetic peptides derived from the variable regions of an anti-CD4 monoclonal antibody bind to CD4 and inhibit HIV-1 promoter activation in virus-infected cells Inra
Monnet, C.; Laune, D.; Laroche-Traineau, J.; Biard-Piechaczyk, M.; Briant, L.; Bès, C.; Pugnière, M.; Mani, J.C.; Pau, B.; Cerutti, M.; Devauchelle, G.; Devaux, C.; Granier, C.; Chardès, T..
Tipo: Journal Article Palavras-chave: HIV; ACTIVATION; CELLULE INFECTEE; ANTICORPS MONOCLONAL; TRANSCRIPTION; IMMUNODEFICIENCE VIRALE.
Ano: 1999 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PUB0400025186106078&uri=/notices/prodinra1/2010/09/
Registros recuperados: 8
Primeira ... 1 ... Última
 

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