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An improved method for artificial infection of the European eel, <i>Anguilla anguilla</i>, with <i>Anguillicola crassus</i> (Nematoda, Dracunculoidea) OMA
De Charleroy, D.; Cannaerts, V.; Augustijn, H.; Grisez, L.; Boon, J.; Ollevier, F.P..
A standardized method was developed for artificially infecting the European eel with <i>Anguillicola crassus</i>, a parasitical swim bladder nematode. For this purpose, an intermediate host, <i>Paracyclops fimbriatus</i>, and a paratenic host, <i>Cyprinus carpio</i>, were used. Parasite prevalence in the artificially infected eels was very high, although there was a large variation in the number of worms per eel. Compared to other methods of artificial infection described in the literature, this method appears to be more efficient ad reliable; there were also more infectious larvae that were recovered and the mortality rate of eels under treatment was lower.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hosts Infestation Invertebrate larvae Methodology Mortality Swim bladder Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus Copepoda [copepods] Cyprinus carpio Nematoda [Nematodes] Paracyclops fimbriatus (Fischer; 1853).
Ano: 1990 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3073
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Comparative serology of the marine fish pathogen <i>Vibrio anguillarum</i> OMA
Grisez, L.; Ollevier, F.P..
The different serotyping systems, based on thermostable O antigens, reported for <i>Vibrio anguillarum</i> and <i>V. ordalii</i> were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within <i>V. anguillarum</i> (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pathogens; Serology; Vibrio anguillarum.
Ano: 1995 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970
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Experimentally induced infections of European eel <i>Anguilla anguilla</i> with <i>Anguillicola crassus</i> (Nematoda, Dracunculoidea) and subsequent migration of larvae OMA
Haenen, O.L.M.; Grisez, L.; De Charleroy, D.; Belpaire, C.; Ollevier, F.P..
Migration patterns of third-stage <i>Anguillicola crassus</i> larvae, and pathogenesis of the lesions induced by third-stage larvae, was investigated in European eel <i>Anguilla anguilla</i> L. Young elvers (1g) were fed infected <i>Paracyclops fimbriatus</i> (Copepoda). Eel samples were collected and examined histologically at varying intervals during 6 mo post-infection period. Third-stage larvae (L-III) migrated directly through the intestinal wall and body cavity to the swimbladder within 17h post-infection. L-IV larvae were detected 3 mo post-infection, and immature adults were detected within 4 mo post-infection. The parasites occasionally showed aberrant migration paths. Pathological effects caused by the parasite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Larvae Life cycle Pathology Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus.
Ano: 1989 URL: http://www.vliz.be/imisdocs/publications/3029.pdf
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Mode of infection and spread of <i>Vibrio anguillarum</i> in turbot <i>Scophthalmus maximus</i> larvae after oral challenge through life feed OMA
Grisez, L.; Chair, M.; Sorgeloos, P.; Ollevier, F.P..
The infection route of the marine fish pathogen <i>Vibrio anguillarum</i> was studied after oral challenge of the juvenile turbot <i>Scophthalmus maximus</i> L. through a life feed. <i>Artemia</i> nauplii were incubated in a suspension of <i>V. anguillarum</i> cells, and subsequently fed twice to the fish. All challenged fish died within 4d after the first challenge, while no mortality occurred in the non-challenged controls. The results of an immunohistochemical examination of the sectioned fish samples clearly demonstrated that <i>V. anguillarum</i> cells were ingested by the <i>Artemia</i> and that the latter were ingested by the fish. Bacteria were released from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scophthalmus maximus (Linneaus; 1758) Vibrio anguillarum.
Ano: 1996 URL: http://www.vliz.be/imisdocs/publications/299074.pdf
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Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals Inra
Bernardet, J.F.; Vancanneyt, M.; Matte-Tailliez, O.; Grisez, L.; Grisez, L.; Tailliez, P.; Bizet, C.; Nowakowski, M.; Kerouault, B.; Swings, J..
Members of most Chryseottacterium species occur in aquatic environments or food products, while strains of some other species are pathogenic to humans and animals. A collection of 52 Chryseobacterium sp. strains isolated from diseased fish, one frog isolate and 22 reference strains were included in a polyphasic taxonomy study. Fourteen clusters of strains were delineated following the comparison of whole-cell protein profiles. Most of these clusters were confirmed when the phenotypic and RAPD profiles and the 16S rRNA gene sequences were compared. Fatty acid composition helped differentiate the Chryseobacterium strains from members of related genera. None of the fish isolates could be allocated to the two species previously reported from fish but two...
Tipo: Journal Article Palavras-chave: MALADIE DES POISSONS CHRYSEOBACTERIUM; ELIZABETHKINGIA; FISH DISEASE; POLYPHASIC TAXONOMY; PHENOTYPIC CHARACTERISTIC; RANDOMLY AMPLIFIED POLYMORPHIC DNA; WHOLE-CELL PROTEIN PROFILE; SDS-PAGE; FATTY ACID ANALYSIS.
Ano: 2005 URL: http://www.prodinra.inra.fr/prodinra/pinra/doc.xsp?id=PROD2009dd4c4130&uri=/notices/prodinra1/2011/05/
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The life cycle of <i>Anguillicola crassus</i> OMA
De Charleroy, D.; Grisez, L.; Thomas, K.; Belpaire, C.; Ollevier, F.P..
For some years now the parasitic swim bladder nematode <i>Anguillicola crassus</i> of the European eel <i>Anguilla anguilla</i> L., has been reported from several European countries. The entire life history of this parasite has recently been elucidated in our laboratory. Young larvae leave the swim bladder of the host via the pneumatic duct and reach the water through the digestive tract. They are ingested by small copepods (Cyclopoida), which act as intermediate hosts. Larvae remain in the hemocoel until the copepods are eaten by the final host, the European eel. Larvae penetrate through the intestinal wall and reach the swim bladder where they develop into adults. When infected copepods are eaten by other small fish, such as carp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological vectors; Disease transmission; Fish diseases; Food chains; Freshwater fish; Hosts; Life cycle; Parasites; Parasitic diseases; Predation; Predators; Swim bladder; Anguillicola crassus.
Ano: 1990 URL: http://www.vliz.be/imisdocs/publications/3074.pdf
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The use of API 20E for the identification of <i>Vibrio anguillarum</i> and <i>V. ordalii</i> OMA
Grisez, L.; Ceusters, R.; Ollevier, F.P..
Sixty-eight strains of <i>Vibrio anguillarum</i>, five of <i>V. ordalii</i> and the type strains of <i>V. alginolyticus, V. carchariae, V. damsela</i> and <i>V. parahaemolyticus</i> were compared using the API 20E gallery. Within the <i>V. anguillarum</i> strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. <i>Vibrio ordalii</i>, the former <i>V. anguillarum</i> biotope 2, could easily be separated from <i>V. anguillarum</i> and from the other fish pathogenic <i>Vibrio</i> spp.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859
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Vibrios associated with routine productions of <i>Brachionus plicatilis</i> OMA
Verdonck, L.; Grisez, L.; Sweetman, E.; Minkoff, G.; Sorgeloos, P.; Ollevier, F.P.; Swings, J..
Surveys for bacteriological analysis were carried out in 1990-1991 in two different marine fish hatcheries located in Greece and northern Spain. Samples were taken from 12 different routine productions of <i>Brachionus plicatilis</i> before and during enrichment. Isolates were characterized by whole cell fatty acid methyl ester analysis and by BIOLOG metabolic fingerprinting. <i>Vibrio anguillarum</i> and/or <i>Vibrio alginolyticus</i> were dominant <i>Vibrio</i> species in the rotifer samples taken after enrichment and rinsing, before being delivered to the fish larvae. None of the <i>Vibrio anguillarum</i> strains belonged to the pathogenic serotypes for juvenile and adult fish.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachionus plicatilis Müller; 1786.
Ano: 1997 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2984
Registros recuperados: 8
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