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Registros recuperados: 5
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Biological characteristics of European hake (Merluccius merluccius) sperm ArchiMer
Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Le Coz, Jean-rene; Jolivet, Aurelie; Garren, Francois.
Very little is known on European hake's reproductive biology and especially on biological characteristics of its sperm despite a growing interest in its aquaculture potential. This study reports, for the first time some hake sperm characteristics. After activation, the swimming phase lasts 3 min (8 min when activated with 50% sea water (SW) but lower initial velocity). The initial flagellar beat frequency (BF), velocity and percentage of motile cells decreased after 100 s. After 2 days at 4 degrees C, the mean Adenylate Energy Charge (AEC) level was 0.71. Sperm stored at 4 degrees C still showed motility after 9 days for 2 individuals. When cryopreserved, the motility recovery index of thawed spermatozoa ranged from 11.8% to 29.6%.
Tipo: Text Palavras-chave: AEC; Gamete quality; Sperm motility; Sperm Density; Merluccius merluccius.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6553.pdf
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Evidence of underestimation of European hake growth in the Bay of Biscay, and its relationship with bias in the agreed method of age estimation ArchiMer
De Pontual, Helene; Groison, Anne-laure; Pineiro, Carmen; Bertignac, Michel.
In 2002, a pilot experiment on hake tagging was carried out using methodology specifically developed to catch and handle fish in,good condition. By the end of 2005, 36 hake and five tags had been returned to the laboratory (a 3.1% return rate) with a maximum time at liberty of 1066 days. The somatic growth of the recoveries proved to be twofold higher than that expected from published von Bertalanffy growth functions for the species in the Bay of Biscay. The growth underestimation was related to age overestimation, as demonstrated by two independent analyses. The first was based on a blind interpretation of marked otoliths conducted independently by two European experts involved in the routine age estimation of hake. The result shows that the age estimates...
Tipo: Text Palavras-chave: Tagging; Otolith; Merluccius merluccius; Growth; European hake; Age validation; Age estimation method; Age.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2221.pdf
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Motility characteristics of spermatozoa in cod (Gadus morhua) and hake (Merluccius merluccius) ArchiMer
Cosson, Jacky; Groison, Anne-laure; Suquet, Marc; Fauvel, Christian.
As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 mu m/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted to the proximal flagellum and tip becomes devoid of wave 3) the wave amplitude decreases. The combination of these factors drastically limits to the earliest period of motility the ability for spermatozoa to efficiently progress towards egg for fertilization.
Tipo: Text Palavras-chave: Osmotic pressure; Sperm motility; Spermatozoa; Hake; Cod.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6552.pdf
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Sexual dimorphism of drumming muscles in European hake (Merluccius merluccius) ArchiMer
Groison, Anne-laure; Kjesbu, Olav S.; Suquet, Marc.
Dissections of mature and non-mature European hake males and females (N=142) collected in waters off the western coast of Norway and in the Bay of Biscay (France) in 2004-2006 demonstrate for the first time that this gadoid species contains drumming muscles. There were differences in drumming muscles weight with body length, sex and maturity stage. This study shows that the difference between females and males is primarily manifested during the spawning season, seen both in the French and Norwegian samples. For the mature females the drumming muscles dry weight increases only slightly, if at all, with increase in total length. For mature males there is a corresponding rapid increase. There does not seem to be any consistent difference between the average...
Tipo: Text Palavras-chave: Drumming muscles; Merluccius merluccius; Sexual dimorphism; Sound production; Spawning.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00036/14681/11983.pdf
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Sperm motility in European hake, Merluccius merluccius, and characterization of its spermatozoa concentration and volume, spermatocrit, osmolality and pH ArchiMer
Groison, Anne-laure; Suquet, Marc; Cosson, Jacky; Mayer, Ian; Severe, Armelle; Bouquet, Jean-marie; Geffen, Audrey J.; Utne-palm, Anne Christine; Kjesbu, Olav Sigurd.
Due to stock declines there is a growing interest in farming of European hake Merluccius merluccius. Thus, knowledge of its sperm biology is of importance not only for purposes of broodstock management, but also for the development of sperm preservation techniques. Hake sperm were collected from mature males caught during the summer-early autumn waters off western Norway and during the winter-early spring in the Bay of Biscay (France). Sperm quality characteristics were assessed after storage at 4 degrees C for 25 +/- 14 h. Average (+/- SD) values for Norwegian and French samples respectively were (i) sperm volume: 3.9 +/- 4.0 and 2.6 +/- 4.0 ml; (ii) spermatozoa concentration: 6.6 +/- 3.2 and 13.9 +/- 5.1 x 10(9) spermatozoa/ml; (iii) spermatocrit: 80.2...
Tipo: Text Palavras-chave: Cryopreservation; Merluccius merluccius; Osmolality; PH; Sperm motility; Spermatocrit.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11393/8021.pdf
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