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Boundaries in metagenomic screenings using lacZα-based vectors Genet. Mol. Biol.
Alves,Luana de Fátima; Borelli,Tiago Cabral; Westmann,Cauã Antunes; Silva-Rocha,Rafael; Guazzaroni,María-Eugenia.
Abstract Metagenomics approaches have been of high relevance for providing enzymes used in diverse industrial applications. In the current study, we have focused on the prospection of protease and glycosyl hydrolase activities from a soil sample by using the lacZα -based plasmid pSEVA232. For this, we used a functional screen based on skimmed milk agar and a pH indicator dye for detection of both enzymes, as previously reported in literature. Although we effectively identified positive clones in the screenings, subsequent experiments revealed that this phenotype was not because of the hydrolytic activity encoded in the metagenomic fragments, but rather due to the insertion of small metagenomic DNA fragments in frame within the coding region of the lacZ...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Functional metagenomics; Protease; Glycosyl hydrolase; False positive clones.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100803
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Genome sequence of the H2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant Genet. Mol. Biol.
Fonseca,Bruna Constante; Riaño-Pachón,Diego Mauricio; Guazzaroni,María-Eugenia; Reginatto,Valeria.
Abstract We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium; Biofuels; Biohydrogen; Beta-glucosidase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100139
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