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PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 Genet. Mol. Biol.
Guo,Qinggang; Li,Shezeng; Lu,Xiuyun; Li,Baoqing; Ma,Ping.
The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus subtilis; Antagonism; Verticillium dahliae; Mini-Tn10.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022
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