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Registros recuperados: 16
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Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis OAK
MIURA, Saori; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; KAWAZU, Shin-ichiro; INOKUMA, Hisashi.
Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
Palavras-chave: Atypical enzootic bovine leukosis; Diagnostic marker; Monoclonal integration.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4143
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Detection of the nonsense mutation of OPA3 gene in Holstein Friesian cattle with dilated cardiomyopathy in Japan OAK
HORIUCHI, Noriyuki; KUMAGAI, Daishiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C>T in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to direct sequences. The homogeneous c.343C>T mutation was proved to occur in all the affected cows and not in the control cows. The present...
Palavras-chave: Cattle; Dilated cardiomyopathy; Nonsense mutation; OPA3 gene.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4208
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Evaluation of Gene Expression in Peripheral Blood Cells as a Potential Biomarker for Enzootic Bovine Leukosis OAK
TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽.
Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers.
Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868
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Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves OAK
KOJIMA, Yuka; CHIBA, Shiori; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 猪熊, 壽.
S100B in cerebrospinal fluid (CSF-S100B) was measured in calves with 20 neurologic and 21 non-neurologic diseases to clarify its utility as a biomarker for neurologic diseases. The median CSF-S100B value in the neurologic disease group (43.0 ng/ml) was significantly higher than that in the non-neurologic disease group (10.2 ng/ml). As CSF-S100B levels in calves with neurologic diseases widely differed, the utility of CSF-S100B as a diagnostic marker for neurologic diseases in cattle remains inconclusive.
Palavras-chave: Calf; Cerebrospinal fluid; S100B.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4142
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Glomus tumor of the liver in a cow OAK
HORIUCHI, Noriyuki; KOMAGATA, Makoto; SHITAMURA, Keiichi; CHIBA, Shiori; MATSUMOTO, Kotaro; INOKUMA, Hisashi; MATSUI, Takane; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古林, 与志安.
An 11-year-old Holstein-Friesian cow exhibited anorexia and jaundice. A large mass was found in the liver during necropsy. Macroscopically, the mass was composed of dark red multilobular tissue and a centrally located abscess, which was connected to the hepatic duct. Histologically, the mass consisted of proliferation of small neoplastic cells and was demarcated from the hepatic parenchyma by a thick region of granulation tissue. The neoplastic cells were predominantly arranged in solid sheets, but they also formed blood-filled cancellous structures, and proliferating foci were seen around blood vessels. Periodic acid-Schiff reaction demonstrated that a fine basement membrane-like structure surrounded the neoplastic cells. Immunohistochemically, the...
Palavras-chave: Cholangitic abscess; Cow; Glomus tumor.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4172
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Immunoreactivity and morphological changes of bursal follicles in chickens infected with vaccine or wild-type strains of the infectious bursal disease virus OAK
AIHARA, Naoyuki; HORIUCHI, Noriyuki; HIKICHI, Nanase; OCHIAI, Mariko; HOSODA, Yuko; ISHIKAWA, Yoko; SHIMAZAKI, Yoko; OISHI, Koji; 堀内, 雅之.
Infectious bursal disease (IBD) is characterized by immunosuppression due to the depletion of lymphocytes in the atrophied bursa of Fabricius (BF). We have sometimes encountered contradictory findings: chickens infected with the vaccine IBD virus (IBDV) strain have sometimes exhibited a highly atrophied BF, but not immunosuppression. In this study, chickens administered vaccine or wild-type strains of IBDV were later vaccinated with the B1 strain of the Newcastle disease virus (NDV). Bursal changes were examined histologically with a focus on the bursal follicle. The immunoreactivity to NDV was also evaluated with the hemagglutination inhibition test. In gross examination, we observed a few chickens with a severely atrophied BF in vaccine...
Palavras-chave: Bursal follicle; Infectious bursal disease; Vaccine.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4148
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Molecular Diagnosis of Infective Endocarditis Caused by Streptococcus canis in a Dog OAK
田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro.
10歳の雄の雑種犬が,3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した.心雑音及び心エコー検査において僧帽弁の疣贅物が確認され,抗生物質等により加療したが,第7病日より心不全の悪化を認め,第10病日に肺水腫により斃死した.病理解剖では,軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた.血液培養検査では,Staphylococcus haemolyticusが検出されたが,疣贅物を用いたPCR検査ではStreptococcus canisと100%一致する塩基配列が得られた.血液培養分離菌はコンタミネーションであった可能性が高く,本症例の心内膜炎はStreptococcus canisによるものと考えられた.血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある.
Palavras-chave: 心内膜炎; PCR; Streptococcus canis.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232
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Optic pathway degeneration in Japanese black cattle OAK
CHIBA, Shiori; FUNATO, Shingo; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of...
Palavras-chave: Axonal degeneration; Gliosis; Japanese black cattle; Optic pathway degeneration.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4123
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Periventricular leukomalacia in a neonatal calf OAK
KOYAMA, Kenji; FUJITA, Riku; MAEZAWA, Masaki; FUKUMOTO, Natsuko; HORIUCHI, Noriyuki; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 猪熊, 壽; 古林, 与志安.
A 10-day-old, Japanese Black, female calf had shown astasia since just after birth. Focal symmetrical periventricular malacic lesions of the cerebrum and suppurative arthritis of the left hip joint were observed in macroscopic examination. Histologically, the cerebral lesions were confirmed as periventricular leukomalacia (PVL). The location and histological features of the lesions were similar to PVL in humans, caused by neonatal ischemia/hypovolemia. This is the first report of PVL in a neonatal calf.
Palavras-chave: Ischemia; Neonatal calf; Periventricular leukomalacia.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4324
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Proliferative Enteropathy Involving Lawsonia intracellularis Infection in Rabbits (Oryctlagus cuniculus) OAK
HORIUCHI, Noriyuki; WATARAI, Masahisa; KOBAYASHI, Yoshiyasu; OMATA, Yoshitaka; FURUOKA, Hidefumi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文.
Five rabbits suffering from diarrhea were diagnosed with proliferative enteropathy (PE). Histopathology revealed a thickened mucosa consisting of hyperplastic intestinal epithelium and infiltration of inflammatory cells mainly consisted of macrophages. In the affected epithelial cytoplasm, numerous curved bacillus-like organisms were observed in the Warthin-Starry silver stain and electron microscopy observation. In polymerase chain reactions, Lawsonia intracellularis-specific DNA fragment were amplified from affected ileal tissue extracted DNA in each case and present 5 cases were confirmed to be L. intracellularis infection. Serum collected from the affected rabbit was immunohistochemically reactive with L. intracellularis in tissue sections from pigs...
Palavras-chave: Lawsonia intracellularis; Proliferative enteropathy; Rabbit.
Ano: 2008 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4169
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Retrospective study of eleven Holstein dairy cows in which bovine leukosis was suspected but ruled out OAK
猪熊, 壽; 堀内, 雅之; 古林, 与志安; INOKUMA, Hisashi; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu.
臨床的に牛白血病を疑ったものの,牛白血病ではないと確定診断されたホルスタイン種乳牛11症例の臨床所見を回顧的に整理した.11症例は体表リンパ節腫大あるいは体腔内の腫瘤触知により牛白血病との鑑別診断が必要であったが,病理解剖により乳房炎,子宮蓄膿症,膿瘍,肺炎,腸炎またはアミロイド症と診断された.牛白血病ウイルス(Bovine leukemia virus: BLV)抗体陽性を呈した4頭のうち,リンパ球増多症を呈したものが2頭であった.またこれら2頭のうち1頭はリンパ節細胞診でも大型リンパ球優勢であった.しかし,血清チミジンキナーゼ(Tymidine kinase: TK),総乳酸脱水素酵素(Lactate dehydrogenase: LDH),LDHアイソザイム2および3の活性値は4頭とも基準値以内であり,牛白血病発症は否定的であった.BLV抗体陰性牛7頭のうち,リンパ節細胞診を実施した2頭は小型リンパ球優勢であり,また,リンパ球増多症を呈した症例はいなかった.1症例では血清TK活性値が基準値をわずかに超えており,また,他の2頭は総LDH,LDHアイソザイム2および3の活性値がいずれも基準値を超えていたが,他の検査項目は牛白血病について否定的であった.腫大リンパ節の細胞診,リンパ球数算定,血清TK,総LDH,LDHアイソザイム2および3の活性値測定といった牛白血病診断のための検査を複数実施することで,牛白血病を否定するための診断精度を高めることが可能と考えられた.
Palavras-chave: 牛白血病; 鑑別診断; 血清チミジンキナーゼ; LDH; LDHアイソザイム.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4367
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Utility of Serum Thymidine Kinase Activity Measurements for Cases of Bovine Leukosis with Difficult Clinical Diagnoses OAK
TAWFEEQ, Mohammad Monir; MIURA, Saori; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽.
This study evaluated the clinical usefulness of serum thymidine kinase (TK) activity for diagnosing bovine leukosis cases for which clinical diagnosis was difficult (‘BL with difficult diagnosis’). Median TK activity values in 24 ‘BL with difficult diagnosis’ and 36 cattle for which BL was clinically confirmed by cytology findings of enlarged superficial lymph nodes (‘clinically confirmed BL’) were 36.8 and 39.4 U/l, respectively (no significant difference). The percentage with positive TK activity (>5.4 U/l) was also similar in both groups (83.3% for ‘BL with difficult diagnosis’ and 97.2% for ‘clinically confirmed BL’). TK activity was significantly higher in cows with ‘BL with difficult diagnosis’ compared to those with other tumors (N=13) and those...
Palavras-chave: Atypical bovine leukosis; Diagnosis; Thymidine kinase.
Ano: 2013 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3869
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左右房室の疣贅性心内膜炎を認めたホルスタイン種乳牛の1症例 OAK
上坂, 花鈴; 藤田, 理公; 水島, 仁人; 堀内, 雅之; 古林, 与志安; 川本, 恵子; 猪熊, 壽; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; KAWAMOTO, Keiko; INOKUMA, Hisashi.
3歳6カ月齢のホルスタイン種乳牛が、分娩2カ月後に食欲不振を主訴に受診した。第6病日には肺音粗励 と心音混濁が確認され、心内膜炎を疑った。第13病日の血液生化学検査では慢性炎症像が確認され、また心音 心電図解析の結果、Ⅰ音分裂と延長、および二峰性P波がみられた。さらに心臓超音波検査では、僧帽弁の疣 贅物と僧帽弁逆流、左心室と左心房の拡張が認められた。第16病日に実施された病理解剖では、僧帽弁に3× 2×2cm 大、三尖弁に3×1×1cm大の疣贅物がそれぞれ確認されたほか、腎梗塞および左肩と左膝の関節 炎がみられた。細菌学的検索では疣贅物、肺、腎臓、関節からは細菌が分離されず、心内膜炎とその他病変と の関係性を解明することができなかった。
Palavras-chave: 疣贅性心内膜炎; 三尖弁; 僧帽弁.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4278
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急性鼻出血を主訴とするホルスタイン種乳牛10症例における臨床所見の病態別比較 OAK
猪熊, 壽; 滄木, 孝弘; 松本, 高太郎; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; MATSUMOTO, Kotaro; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
Ano: 2015 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4145
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胸椎椎体膿瘍により後躯麻痺を呈したホルスタイン種子牛の1症例 OAK
河野, 友美; 菊地, 智景; 鬼頭, 宗寛; 堀内, 雅之; 古林, 与志安; 山田, 一孝; 猪熊, 壽; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisahi.
3カ月齢のホルスタイン種雌子牛が後躯麻痺、起立不能、努力性呼吸を呈した。デキサメサゾン投与により一時的に自力起立が可能になったが、後躯蹌踉は改善しなかった。神経学的検査により後肢の反射亢進が認められ、第3胸椎(T3)~第3腰椎(L3)の脊髄病変を疑った。X線検査およびCT検査によりT5‐T6の椎体変形および椎体膿瘍形成が認められたので、生前診断に至った。病理解剖により、この胸椎腫瘤は椎間円盤と椎体を侵襲し、脊椎管内に突出して脊髄を圧迫していた。また肺にも膿瘍形成が認められたが、細菌検査では細菌が分離されなかったため、椎体膿瘍、肺膿瘍の関連性は不明であった。
Palavras-chave: 子牛; X線検査; CT検査; 後躯麻痺; 椎体膿瘍.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4391
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膣内腫瘤の細胞診が臨床診断に有用であった黒毛和種繁殖牛の地方病性牛白血病の1症例 OAK
前澤, 誠希; 大戸, 千帆; 関塚, 次郎; 堀内, 雅之; 古林, 与志安; 猪熊, 壽; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Palavras-chave: 膣内腫瘤; 細胞診; 地方病性牛白血病.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4259
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