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Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays BABT
Hashimoto,Elisabete Hiromi; Kamogae,Márcia; Vanzella,Tatiana Perez; Cólus,Ilce Mara Syllus; Bracarense,Ana Paula Frederico Rodrigues Lourenço; Bittencourt-Oliveira,Maria do Carmo; Itano,Eiko; Kuroda,Emília Kiyomi; Kato,Hajime; Nagata,Satoshi; Ueno,Yoshio; Harada,Ken-Ichi; Hirooka,Elisa Yoko.
This work investigated the effects of co-occurring aflatoxin B1 (AFB1) and microcystin (MC) in aquaculture, using immunohistochemistry and genotoxicity methods. Tilapia (Oreochromis niloticus) were exposed to AFB1 by intraperitoneal and MC (cell extract of Microcystis aeruginosa) by intraperitoneal and immersion routes. The interaction of MC-AFB1 was evaluated co-exposing the intraperitoneal doses. Blood samples were collected after 8, 24, and 48h to analyze the micronucleus frequency and comet score. The interaction of MC-AFB1 showed a synergic mutagenic response by higher micronucleus frequency of co-exposed group. A slight genotoxic synergism was also observed in the comet score. Immunohistochemistry detected MC in al lthe fish liver tissues exposed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microcystis aeruginosa; Mycotoxin and tilapia.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100019
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β-Glucanase Addition in Brewing Malt Produced by Reduced Time of Germination BABT
Brazil,Crislane; Oliveira,Débora Francielly de; Duarte,Rafael Augusto; Galo,Juliana Minardi; Lucchetta,Luciano; Santos,Eder da Costa dos; Hashimoto,Elisabete Hiromi.
Abstract The β-Glucans content has straight influence on the quality of malt and beer, mainly during the filtration step. Barley presenting high β-Glucan content demands longer germination time at malting. The application of commercial β-Glucanase is an alternative to accelerate the process and preserve the quality of malt. This work aimed to evaluate the effect of commercial β-Glucanase addition in malt produced within reduced germination time (64 h). Micro-malting was conducted with BRS-Caue and Elis barley cultivars at germination time 64 h and 96 h. The β-Glucanase concentration applied were 0, 25, 50 and 100 mg.kg-1. Barley, malt and wort samples were analyzed to check their physical-chemical features. Beers were produced with BRS-Caue malt and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-glucans; Germination time; Commercial enzyme.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100222
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