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Registros recuperados: 79
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A mussel (Mytilus edulis) tissue certified reference material for the marine biotoxins azaspiracids ArchiMer
Mccarron, Pearse; Giddings, Sabrina D.; Reeves, Kelley L.; Hess, Philipp; Quilliam, Michael A..
Azaspiracids (AZAs) are lipophilic biotoxins produced by marine algae that can contaminate shellfish and cause human illness. The European Union (EU) regulates the level of AZAs in shellfish destined for the commercial market, with liquid chromatography-mass spectrometry (LC-MS) being used as the official reference method for regulatory analysis. Certified reference materials (CRMs) are essential tools for the development, validation, and quality control of LC-MS methods. This paper describes the work that went into the planning, preparation, characterization, and certification of CRM-AZA-Mus, a tissue matrix CRM, which was prepared as a wet homogenate from mussels (Mytilus edulis) naturally contaminated with AZAs. The homogeneity and stability of...
Tipo: Text Palavras-chave: Azaspiracids; Seafood safety; Matrix certified reference material; Liquid chromatography-mass spectrometry; Matrix effects.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00320/43138/42761.pdf
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A mussel tissue certified reference material for multiple phycotoxins. Part 1: design and preparation ArchiMer
Mccarron, Pearse; Emteborg, Hakan; Nulty, Ciara; Rundberget, Thomas; Loader, Jared I.; Teipel, Katharina; Miles, Christopher O.; Quilliam, Michael A.; Hess, Philipp.
The development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography-mass spectrometry permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness. However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier studies as...
Tipo: Text Palavras-chave: CRM-FDMT1; Certified reference material; Shellfish toxins; Phycotoxins; Accuracy; Precision; Liquid chromatography-mass spectrometry.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00037/14826/12237.pdf
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Acute Oral Toxicity of Pinnatoxin G in Mice ArchiMer
Sosa, Silvio; Pelin, Marco; Cavion, Federica; Herve, Fabienne; Hess, Philipp; Tubaro, Aurelia.
Pinnatoxin G (PnTx-G) is a marine cyclic imine toxin produced by the dinoflagellate Vulcanodinium rugosum, frequently detected in edible shellfish from Ingril Lagoon (France). As other pinnatoxins, to date, no human poisonings ascribed to consumption of PnTx-G contaminated seafood have been reported, despite its potent antagonism at nicotinic acetylcholine receptors and its high and fast-acting toxicity after intraperitoneal or oral administration in mice. The hazard characterization of PnTx-G by oral exposure is limited to a single acute toxicity study recording lethality and clinical signs in non-fasted mice treated by gavage or through voluntary food ingestion, which showed differences in PnTx-G toxic potency. Thus, an acute toxicity study was carried...
Tipo: Text Palavras-chave: Vulcanodinium rugosum; Pinnatoxin G; Dinoflagellates; Harmful algae; Oral toxicity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00605/71724/70190.pdf
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Algal toxin discovery, management and regulation over the last 25 years ArchiMer
Hess, Philipp.
Tipo: Text
Ano: 2018 URL: http://archimer.ifremer.fr/doc/00450/56180/57728.pdf
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Algal toxin profiles in Nigerian coastal waters (Gulf of Guinea) using passive sampling and liquid chromatography coupled to mass spectrometry ArchiMer
Zendong, Suzie Zita; Kadiri, Medina; Herrenknecht, Christine; Nezan, Elisabeth; Mazzeo, Antonia; Hess, Philipp.
Algal toxins may accumulate in fish and shellfish and thus cause poisoning in consumers of seafood. Such toxins and the algae producing them are regularly surveyed in many countries, including Europe, North America, Japan and others. However, very little is known regards the occurrence of such algae and their toxins in most African countries. This paper reports on a survey of phytoplankton and algal toxins in Nigerian coastal waters. Seawater samples were obtained from four sites for phytoplankton identification, on three occasions between the middle of October 2014 and the end of February 2015 (Bar Beach and Lekki in Lagos State, Port Harcourt in Rivers State and Uyo in Akwa Ibom State). The phytoplankton community was generally dominated by diatoms and...
Tipo: Text Palavras-chave: Dinoflagellates; Dinophysis; Phycotoxins; Untargeted analysis; Phytoplankton.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00314/42521/41893.pdf
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Assessment and management of biotoxin risks in bivalve molluscs ArchiMer
Lawrence, Jim; Loreal, Henri; Toyofuku, Hajime; Hess, Philipp; Iddya, Karunasagar; FAO.
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00085/19588/17212.pdf
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Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum ArchiMer
Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum. Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent...
Tipo: Text Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf
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Azaspiracid shellfish poisoning: A review on the chemistry, ecology, and toxicology with an emphasis on human health impacts ArchiMer
Twiner, Michael J.; Rehmann, Nils; Hess, Philipp; Doucette, Gregory J..
Azaspiracids (AZA) are polyether marine toxins that accumulate in various shellfish species and have been associated with severe gastrointestinal human intoxications since 1995. This toxin class has since been reported from several countries, including Morocco and much of western Europe. A regulatory limit of 160 mu g AZA/kg whole shellfish flesh was established by the EU in order to protect human health; however, in some cases, AZA concentrations far exceed the action level. Herein we discuss recent advances on the chemistry of various AZA analogs, review the ecology of AZAs, including the putative progenitor algal species, collectively interpret the in vitro and in vivo data on the toxicology of AZAs relating to human health issues, and outline the...
Tipo: Text Palavras-chave: Azaspiracid (AZA); AZP; Shellfish poisoning.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/00077/18805/16375.pdf
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Azaspiracid Toxins: Toxicological Profile ArchiMer
Hess, Philipp; Twiner, Michael J; Kilcoyne, Jane; Sosa, Sylvio.
Azaspiracids (AZAs) are a toxin group that originate from marine dinoflagellates of the genera Azadinium and Amphidoma. After accumulation of these toxins in edible marine organisms and their subsequent consumption, humans develop a gastrointestinal syndrome referred to as azaspiracid shellfish poisoning (AZP). This syndrome is very similar to diarrheic shellfish poisoning (DSP), with main symptoms appearing after a few hours from consumption and including diarrhea, vomiting, and stomach cramps. Due to extensive metabolism in shellfish, more than 30 analogues have been reported to date, and purified compounds for selected analogues have recently been made available for toxicological studies. Currently, only AZA1, AZA2, and AZA3 are regulated in Europe and...
Tipo: Text Palavras-chave: Azaspiracids; Azaspiracid shellfish poisoning; Harmful algae; Azadinium; Shellfish.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00320/43146/42744.pdf
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Azaspiracids Increase Mitochondrial Dehydrogenases Activity in Hepatocytes: Involvement of Potassium and Chloride Ions ArchiMer
Pelin, Marco; Kilcoyne, Jane; Florio, Chiara; Hess, Philipp; Tubaro, Aurelia; Sosa, Silvio.
Background: Azaspiracids (AZAs) are marine toxins that are produced by Azadinium and Amphidoma dinoflagellates that can contaminate edible shellfish inducing a foodborne poisoning in humans, which is characterized by gastrointestinal symptoms. Among these, AZA1, -2, and -3 are regulated in the European Union, being the most important in terms of occurrence and toxicity. In vivo studies in mice showed that, in addition to gastrointestinal effects, AZA1 induces liver alterations that are visible as a swollen organ, with the presence of hepatocellular fat droplets and vacuoles. Hence, an in vitro study was carried out to investigate the effects of AZA1, -2, and -3 on liver cells, using human non-tumor IHH hepatocytes. Results: The exposure of IHH cells to...
Tipo: Text Palavras-chave: Azaspiracids; Hepatocytes; Mitochondrial activity; Mechanism of toxicity.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00494/60526/63989.pdf
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Beta-N-Methylamino-L-Alanine: LC-MS/MS Optimization, Screening of Cyanobacterial Strains and Occurrence in Shellfish from Thau, a French Mediterranean Lagoon ArchiMer
Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Brient, Luc; Savar, Veronique; Bardouil, Michele; Hess, Philipp; Amzil, Zouher.
beta-N-methylamino-L-alanine (BMAA) is a neurotoxic non-protein amino acid suggested to be involved in neurodegenerative diseases. It was reported to be produced by cyanobacteria, but also found in edible aquatic organisms, thus raising concern of a widespread human exposure. However, the chemical analysis of BMAA and its isomers are controversial, mainly due to the lack of selectivity of the analytical methods. Using factorial design, we have optimized the chromatographic separation of underivatized analogues by a hydrophilic interaction chromatography coupled to tandem mass spectrometry (HILIC-MS/MS) method. A combination of an effective solid phase extraction (SPE) clean-up, appropriate chromatographic resolution and the use of specific mass spectral...
Tipo: Text Palavras-chave: Cyanotoxins; BMAA; DAB; AEG; HILIC-MS/MS; Cyanobacteria; Bivalve mollusks; French Mediterranean.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00245/35579/34112.pdf
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Characterization of ovatoxin-h, a new ovatoxin analog, and evaluation of chromatographic columns for ovatoxin analysis and purification ArchiMer
Brissard, Charline; Herve, Fabienne; Sibat, Manoella; Sechet, Veronique; Hess, Philipp; Amzil, Zouher; Herrenknecht, Christine.
The presence of Ostreopsis cf. ovata on the Mediterranean coast represents a serious concern to human health due to production of toxins–putative palytoxin and ovatoxins (ovatoxin-a, -b, -c, -d, -e, -f and -g). However, purified ovatoxins are not widely available and their toxicities are still unknown. In the present study, we report on HR LC-MS/MS analysis of a French Ostreopsis cf. ovata strain (IFR-OST-0.3 V) collected at Villefranche-sur-Mer (France) during a bloom in 2011. Investigation of this strain of Ostreopsis cf. ovata cultivated in our laboratory by ultra-high performance liquid chromatography coupled to high resolution mass spectrometry (UHPLC-HRMS) confirmed the production of ovatoxins -a to–e and revealed the presence of a new ovatoxin...
Tipo: Text Palavras-chave: Ostreopsis cf. ovata; Palytoxin; Ovatoxins; U-HPLC/HR-MSn; Chromatographic separation.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00252/36302/34831.pdf
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Chemically mediated interactions between Microcystis and Planktothrix : impact on their growth, morphology and metabolic profiles ArchiMer
Briand, Enora; Reubrecht, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam.
Freshwater cyanobacteria are known for their ability to produce bioactive compounds, some of which have been described as allelochemicals. Using a combined approach of co‐cultures and analyses of metabolic profiles, we investigated chemically mediated interactions between two cyanobacterial strains, Microcystis aeruginosa PCC7806 and Planktothrix agardhii PCC7805. More precisely, we evaluated changes in growth, morphology and metabolite production and release by both interacting species. Co‐culture of Microcystis with Planktothrix resulted in a reduction of the growth of Planktothrix together with a decrease of its trichome size and alterations in the morphology of its cells. The production of intracellular compounds by Planktothrix showed a slight...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00470/58152/60632.pdf
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Chemically mediated interactions between Microcystis and Planktothrix: Impact on their physiology and metabolic profiles ArchiMer
Briand, Enora; Reubrech, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam.
Freshwater cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. We tested if the production of a range of peptides by co-existing species could be regulated through interspecific interactions. Using a combined approach of co-cultures and analyses...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00374/48515/48794.pdf
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Ciguatoxicity of Gambierdiscus and Fukuyoa species from the Caribbean and Gulf of Mexico ArchiMer
Litaker, R. Wayne; Holland, William C.; Hardison, D. Ransom; Pisapia, Francesco; Hess, Philipp; Kibler, Steven R.; Tester, Patricia A..
Dinoflagellate species belonging to the genera Gambierdiscus and Fukuyoa produce ciguatoxins (CTXs), potent neurotoxins that concentrate in fish causing ciguatera fish poisoning (CFP) in humans. While the structures and toxicities of ciguatoxins isolated from fish in the Pacific and Caribbean are known, there are few data on the variation in toxicity between and among species of Gambierdiscus and Fukuyoa. Quantifying the differences in species-specific toxicity is especially important to developing an effective cell-based risk assessment strategy for CFP. This study analyzed the ciguatoxicity of 33 strains representing seven Gambierdiscus and one Fukuyoa species using a cell based Neuro-2a cytotoxicity assay. All strains were isolated from either the...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00407/51839/52439.pdf
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COLNACOQ. Composés lipophiles naturels dans l’environnement des coquillages. Rapport final ArchiMer
Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois.
Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques...
Tipo: Text Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf
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Combined oral toxicity of azaspiracid-1 and yessotoxin in female NMRI mice ArchiMer
Aasen, John A. B.; Espenes, Arild; Miles, Christopher O.; Samdal, Ingunn A.; Hess, Philipp; Aune, Tore.
For many years, the presence of yessotoxins (YTXs) in shellfish has contributed to the outcome of the traditional mouse bioassay and has on many occasions caused closure of shellfisheries. Since YTXs do not appear to cause diarrhoea in man and exert low oral toxicity in animal experiments, it has been suggested that they should be removed from regulation. Before doing so, it is important to determine whether the oral toxicity of YTXs is enhanced when present together with shellfish toxins known to cause damage to the gastrointestinal tract. Consequently, mice were given high doses of YTX, at 1 or 5 mg/kg body weight, either alone or together with azaspiracid-1 (AZA1) at 200 μg/kg. The latter has been shown to induce damage to the small intestine at this...
Tipo: Text Palavras-chave: Azaspiracid-1; AZA1; Yessotoxin; YTX; Marine algal toxins; Absorption; Pathology; Sublethal; NMRI; Mice; LC-MS/MS; Oral toxicity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00034/14477/11776.pdf
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Complex Toxin Profile of French Mediterranean Ostreopsis cf. ovata Strains, Seafood Accumulation and Ovatoxins Prepurification ArchiMer
Brissard, Charline; Herrenknecht, Christine; Sechet, Veronique; Herve, Fabienne; Pisapia, Francesco; Harcouet, Jocelyn; Lemee, Rodolphe; Chomerat, Nicolas; Hess, Philipp; Amzil, Zouher.
Ostreopsis cf. ovata produces palytoxin analogues including ovatoxins (OVTXs) and a putative palytoxin (p-PLTX), which can accumulate in marine organisms and may possibly lead to food intoxication. However, purified ovatoxins are not widely available and their toxicities are still unknown. The aim of this study was to improve understanding of the ecophysiology of Ostreopsis cf. ovata and its toxin production as well as to optimize the purification process for ovatoxin. During Ostreopsis blooms in 2011 and 2012 in Villefranche-sur-Mer (France, NW Mediterranean Sea), microalgae epiphytic cells and marine organisms were collected and analyzed both by LC-MS/MS and hemolysis assay. Results obtained with these two methods were comparable, suggesting ovatoxins...
Tipo: Text Palavras-chave: Ostreopsis cf. ovata; Palytoxin & ovatoxins; Culture; Seafood contamination; LC-MS/MS; Hemolysis assay; Mediterranean Sea.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00199/31025/29428.pdf
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Contribution of marine invertebrates to Ciguatera poisoning : the case study of French Polynesia ArchiMer
Darius, Ht; Viallon, J; Gatti, Cm; Chinain, M; Roué, M; Sibat, Manoella; Amzil, Zouher; Hess, Philipp; Vandersea, Mw; Litaker, Rw; Tester, Pa.
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00450/56181/57729.pdf
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Contributions to the characterisation of risks posed by marine biotoxins ArchiMer
Hess, Philipp.
Toxic algae producing an array of bioactive compounds may accumulate in shellfish, which in turn cause poisoning, following human consumption of contaminated shellfish. The chemistry, occurrence and toxicity of the toxins involved was reviewed. Following this review, the risk analysis and management processes are outlined for the production of shellfish. Subsequent sections describe five elements of risk characterisation to which my studies have significantly contributed: methods of analysis, distribution of toxins in the marine environment, preparative isolation of toxins, quality control tools in the determination of the toxins and characterisation of the chemical hazard in terms of lipophilicity, stability, reactivity and toxicity. The use of liquid...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12661/9548.pdf
Registros recuperados: 79
Primeira ... 1234 ... Última
 

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