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A flow cytometric approach to study intracellular-free Ca2+ in Crassostrea gigas haemocytes ArchiMer
Aton, Emilie; Renault, Tristan; Gagnaire, Beatrice; Thomas Guyon, Hélène; Cognard, C; Imbert, N.
Bivalve haemocytes are essential in defence mechanisms including phagocytosis. They also produce molecules including hydrolytic enzymes and antimicrobial peptides that contribute to pathogen destruction. Although haemocyte activities have been extensively studied, relatively little is known about the intracellular signalling pathways that are evoked during haemocyte activation and especially the role of calcium. Flow cytometry has been used for the first time to define the effect of cell incubation in haemolymph and artificial sea water (ASW) on Pacific oyster, Crassostrea gigas, haemocytes. Cell viability, enzymatic activities (esterases and amino peptidases), phagocytosis and granulocyte percentage were analysed. Viability and some activities were...
Tipo: Text Palavras-chave: Cellular activities; Fluo 3/AM; Calcium; Flow cytometry; Haemocytes; Crassostrea gigas.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-935.pdf
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