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Registros recuperados: 5
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A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee BABT
Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020
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Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain PAB
Silva,André Ribeiro da; Zanin,Lívia Montanheiro Médici; Ishikawa,Angélica Tieme; Yamashita,Cassia Reika Takabayashi; Fracalossi,Felipe Pedote; Amorim,Thaís Marques; Itano,Eiko Nakagawa; Kawamaura,Osamu; Hirooka,Elisa Yoko.
Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus; Aflatoxin B1; Blanched peanuts; Contamination; Mycotoxin.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300361
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Ecological aspects of Bacillus thuringiensis in an Oxisol Scientia Agricola
Ferreira,Lessandra Heck Paes Leme; Suzuki,Marise Tanaka; Itano,Eiko Nakagawa; Ono,Mário Augusto; Arantes,Olívia Márcia Nagy.
Bacillus thuringiensis is a Gram positive, sporangial bacterium, known for its insecticidal habilities. Survival and conjugation ability of B. thuringiensis strains were investigated; vegetative cells were evaluated in non-sterile soil. Vegetative cells decreased rapidly in number, and after 48 hours the population was predominantly spores. No plasmid transfer was observed in non-sterile soil, probably because the cells died and the remaining cells sporulated quickly. Soil is not a favorable environment for B. thuringiensis multiplication and conjugation. The fate of purified B. thuringiensis toxin was analyzed by extractable toxin quantification using ELISA. The extractable toxin probably declined due to binding on surface-active particles in the soil.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Population dynamics; Insecticidal crystal protein; Conjugation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100004
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Immunoglobulin G proteolytic activity of Actinobacillus actinomycetemcomitans BJM
Nakanishi,Fernanda Akemi; Avila-Campos,Mario Julio; Kamiji,Nádia Hizuru; Itano,Eiko Nakagawa.
Actinobacillus actinomycetemcomitans produces a protease to human immunoglobulin G that is an important evasion mechanism. In this study, the proteolytic activity of A. actinomycetemcomitans strain ATCC 43718 on human immunoglobulin G associated with culture supernatant concentrations, the growth period and the period of incubation with immunoglobulin G were evaluated by an enzyme linked immunosorbent assay. The protease fraction was detected by Sephadex G 150 chromatography. The results showed that A. actinomycetemcomitans produced a protease to human immunoglobulin G in the culture supernatant, and the highest activity was achieved witen the concentration was 27.5 mug protein/mL, after culturing for 72 hours and incubating with IgG for 24 hours. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunoglobulin G; Actinobacillus actinomycetemcomitans; ELISA; Protease.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100008
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The effects of nitric oxide on the immune response during giardiasis BJID
Pavanelli,Wander Rogério; Gutierrez,Fredy Roberto Salazar; Silva,Jean Jerley Nogueira da; Costa,Ivete Conchon; Menezes,Maria Claudia Noronha Dutra de; Oliveira,Francisco José de Abreu; Itano,Eiko Nakagawa; Watanabe,Maria Angélica Ehara.
Nitric oxide (NO) is a free radical synthesized from L-arginine by different isoforms NO-synthases. NO possesses multiple and complex biological functions. NO is an important mediator of homeostasis, and changes in its generation or actions can contribute or not to pathological states. The knowledge of effects of NO has been not only important to our understanding of immune response, but also to new tools for research and treatment of various diseases. Knowing the importance of NO as inflammatory mediator in diverse infectious diseases, we decided to develop a revision that shows the participation/effect of this mediator in immune response induced against Giardia spp. Several studies already demonstrated the participation of NO with microbicidal and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide; Immune system; Giardiasis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000600010
Registros recuperados: 5
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