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不同廠牌及種類之膠體化物質對水稻花藥培養之影響 Taiwan Agricultural Research Institute
高小玲; 葉常青; 許家言; 蔡新聲; S.L. Gau; C.C. Yeh; J.Y. Hsu; H.S. Tsay.
[[abstract]]將水稻花藥培養於五種不同品牌之agar時,結果顯示以Difco-Bacto agar(No. 0140─01)對水稻花藥癒合組織的形成及分化較優,而惠光出品之agar表現最差。 不同固體化介質以agarose最好,可以顯著提高水稻癒合組織形成率及綠苗分化率,其次為starch, gelrite雖可提高癒合組織形成率及綠苗分化率但與agar差異不大。增加corn starch濃度或以rice starch培養水稻花藥,均可提高癒合組織形成率及分化率。在agar的培養基內以不同比例之corn starch取代,隨著corn starch取代量的增加,癒合組織形成率及分化率均有漸增的趨勢。 A series of experiments were taken to compare the effects of five agar brands (Sigma, Difco-Bacto No. 0140-01, Difco-Bitek No. 0138-01-4, BBL and Huey-Guang) and different gelling agents and concentrations on callus induction and plant regeneration in rice anther culture. In the comparison of the five agar brands, the best results on callus and plant differentiation was obtained with Difco-Bacto agars while the addition of Huey-Guang agar to the medium...
Palavras-chave: [[classification]]6.
Ano: 1990
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低溫處理對水稻花藥接種適期之影響 Taiwan Agricultural Research Institute
蔡新聲; 陳駿季; 葉常青; 許家言; H.S. Tsay; J.J. Chen; C.C. Yeh; J.Y. Hsu.
[[abstract]]水稻稻穗經不同時間之低溫(10°C)處理後,選取含四分子期至晚單核期小孢子之花藥進行培養,探討低溫處理對花藥接種適期之影響。結果發現,各時期小孢子對低溫處理的反應差異極大。經七天之低溫處理,以中單核期小孢子之花藥最易形成癒合組織,且其誘導率較經低溫處理一天者提高一倍左右。低溫處理14天,可提高晚單核期小孢子之花藥形成癒合組織之能力,但對中單核期之花藥癒合組織誘導率則無提高之效果,過長之低溫處理(超過21天)反會抑制癒合組織之產生。 七天之低溫處理,會導致由四分子期小孢子之花藥所誘導的癒合組織喪失分化能力,短期(1─7天)之低溫處理,由中、晚單核期小孢子之花藥所形成的癒合組織,分化率有提高之現象,但較容易分化成白苗。超過14天之低溫處理,綠苗分化能力則顯著降低。 就育種效率而言,低溫處理七天以內,接種中單核期小孢子之花藥可得較高之育種效率;低溫處理14天則以接種晚單核期小孢子之花藥為佳,但其所分化綠株多為單倍體。超過21天之低溫處理,任何時期小孢子之花藥育種效率皆低於3%,而不適合進行培養。本試驗同時以二種貯存方式進行低溫處理,結果顯示,定期更換包裹於葉鞘基部之衛生紙,有助於花藥在長期低溫處理下維持較佳的活力。 The base of rice panicles with microspores at different developmental stages were wrapped with moist tissue paper and kept at 10°C for various durations to test the effect of cold shock on callus formation, organ regeneration and chromosome...
Palavras-chave: [[classification]]6.
Ano: 1988
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